MX2008010089A - Sample vessel for accommodating small amounts of liquid for analyses - Google Patents

Abstract

In a sample vessel (1) for accommodating small amounts of liquid for analyses, consisting of a tube (2) closed at one end and closable with sealing at the other open end by a closure cap (3) which can be pierced by a tip of a withdrawal cannula, the closure cap (3) is designed with an externally flat, even top wall (5) and a membrane disk (7) which is inserted from the bottom so as to rest on the inner surface of the top wall (5) and is held supported there.

Description

CONTAINERS FOR SAMPLES TO CONTAIN SMALL QUANTITIES OF LIQUIDS FOR ANALYSIS.

FIELD OF THE INVENTION The invention refers to a container for samples to contain small amounts of liquid for analysis, consisting of a small tube that on one side is closed and at its other open end can be closed by means of a hermetic cover that it can be perforated by means of the tip of a cannula extractor. BACKGROUND OF THE INVENTION These test tubes or sample containers in different modalities are known from DE 103 40 538 A1. The lid that closes the open end is a pierceable plug, which extends with a cylindrical sealing section at the open end of the small tube and there forms a seal with the inner wall of the small tube. They are suitable for the completely adequate extraction of samples of analysis, inside the plug of finds placed an insert inside the plug consisting of rubber / rubber or an elastomeric synthetic material that is mounted from above and that can be punctured by the tip of a cannula extractor This insert consisting of a material that recovers its shape completely elastically can be perforated directly through the cannula of the analyzer apparatus, whereby the perforation hole formed temporarily closes completely when the cannula is removed. Another cover is known from EP 0445 707 Bl. In closure device for the open end of a sample container, especially a blood test tube, includes a lid with a sealing cap mounted from above. To securely hold the sealing cap on the lid, a clamping ring or a securing disk is mounted on it from the free outer front side. The closed systems so called especially when working with samples consisting of materials of biological origin, since they reduce the danger of infection for the user (for example by transfer of pathological germs) or reduce contamination of the probe by the user ( for example molecular biological contamination). The samples are characterized and often are transported frozen long distances. The characterization of the sample ideally must be done in such a way that it is also visible when the sample containers are stored tightly in supports. Closed systems that belong to the state of the téncica are used for example for the field of obtaining or extracting blood and include primary containers, which receives large quantities of blood, at least 2 ml, and in most cases larger volumes. In the case of direct adaptation of the closed systems, small sample quantities of only a few μ? . In these primary containers, it is also known to provide screw-on plugs with a perforable and resealable membrane (see DE 30 49 503 C). This known cover, which closes the front end of the extraction tubes, has a cylindrical dome protruding in the axial direction, which has a pierceable stopper mounted from above, which is located on a front plate of the dome provided with a medium perforation, and which It is held by a neck that surrounds the edge. This protrusion extends in such a way that it allows the surface of the cover to be provided with a label or with the name of the sample. No described closed systems of primary containers with large volumes are available for handling samples with sample quantities in secondary containers that are clearly less than 2 ml. For the analysis in chromatography systems it is usual to transfer the sample and the reagent from the original container by means of a pipette with this open the container for analysis of the system of chromatography In the case of molecular biology works, the test works must be opened repeatedly to introduce a reagent or to extract a part of the sample. The three systems mentioned have the disadvantage that the plugs produced from elastomeric material, they are placed and held from above, this is from the front side. This does not form a flat front surface on the lid that can be used to identify the sample. SUMMARY OF THE INVENTION Therefore the task proposes the task of producing a container for samples of the aforementioned type that makes possible a closed system also for the case of small amounts of liquid and especially that simultaneously allows a visible identification of the sample . This task is solved according to the invention because the cover cap is formed with an external uniform flat upper wall and with a membrane placed and resting on the inner surface of the upper wall. This results in a lid that adapts to the sample containers, which can be made in the form of a screw cap or pressure cap, and in which the surface The outside of the lid or the upper wall is available unlimitedly, to be able to provide a label with a folio or similar without problems as was inevitable in the case of the membrane used with a correspondingly large hole. The perforable membrane placed from below or from inside is accessible unimpeded from outside for a cannulae extractor, possibly through a small hole-penetration with a reduced diameter, which allows an entry or exit of samples and reagents, and the which also in the case of a prolonged repetition of an extraction process the membrane can not be expelled from the lid. Since it is held fixed in its position on the lid by means of a held bra. This fixed seating in the position or support and support of the membrane can for example be achieved because an edge bent inwardly of an inner neck of the lid and / or a perforated plate placed on the inner neck and held by it holds the membrane. In this case, the membrane, which is preferably of rubber, can not be expelled from the inner neck when exerting a downward pressure. The flange can advantageously be produced when the end edge of the neck is heat-molded internal An advantageous embodiment of the invention provides that the upper wall of the lid is provided with a synthetic membrane covering the bore hole-passage of the upper wall of the lid. Also in the case of this embodiment, the lid has a flat, uniform outer surface without limitations to identify the sample and by means of the thin film at the same time it is provided with a protection against contamination. BRIEF DESCRIPTION OF THE FIGURES Other features and characteristics of the invention are apparent from the following description of embodiments of the invention represented in the drawings on a greatly enlarged scale. Figure 1 shows a longitudinal section of a total view of a sample container for small quantities of liquids, closed by means of a hermetic cover, in this case a screw cap, with a perforable membrane placed and fastened inside; and Figure 2 shows a particularity of a longitudinal section of another embodiment of the screw cap.

DETAILED DESCRIPTION OF THE INVENTION The sample container 1 represented in figure 1 consists of a small tube 2 whose lower end is previously closed, to contain small amounts of liquids. At its upper end the small tube 2 is temporarily closed by means of a sealing screw cap 3, hereinafter referred to as a screw cap. The screw cap 3 has an internal neck 4 and in its upper wall 5 a perforation orifice 6 of small dimensions concentric to the inner neck. From inside or below, a pierceable membrane 7 fastened to the inner neck 4 is placed on or inside the screw cap 3, resting on the inner surface of the upper wall 5. This is fixedly fixed in its position by means of the heat deformation of the edge 8 of the inner neck 4 that surrounds it. In order to seal the screw cap 3, an air-tight flange can be provided, which guarantees the airtight seating of the lid even in the event of a possible overpressure, which can be formed, for example, when the reagents are added.

Alternatively, it is possible to obtain a liquid-tight seal when using, for example, O-rings, sealing lips, sealing plates or the like. The screw cap shown in the figure 2 differs from the embodiment described above in that the drill hole-step 6 is covered with a thin synthetic membrane 10, which has the thickness of a film which, for example, amounts to 0.1 mm. The threaded caps 3 have in common that they cover a small tube 2 for small quantities of samples, which do not have to be opened for a repeated extraction of a part or a partial quantity of the sample or for the repeated addition of reagents. The mounting position of the highly elastic membrane 7flexible and easily pierceable by a cannula, does not lose its tightness by means of the repeated penetration and extraction of a cannula or a sample extractor needle, and the membrane 8 can not be detached under pressure from the screw cap 3. Especially by means of the assembly of the membrane 7 from below, a flat and uniform outer side of the upper wall is formed and therefore a sufficient surface area is available great for the smooth application of sample identification. This also applies to a type of screw cap with a drill hole, which can be covered by means of a synthetic membrane.

Claims (1)

NOVELTY OF THE INVENTION Having described the invention as above, property is claimed as contained in the following: CLAIMS

1. A container for samples (1) to contain small amounts of liquid for analysis, consisting of a small tube (2) closed at one end, and a lid (3) of a single piece that has a membrane (7) that can be pierced by the tip of an extraction cannula and that hermetically closes the other end that is open of the tube, characterized in that the lid (3) is formed by an upper wall (5) and a membrane (7) placed from below that is held supported from below, and that rests on the internal surface of the upper wall (5).