MD940033A - Procedeu de investigaţie imunoenzimatică omogenă şi trusă pentru realizarea lui - Google Patents

Procedeu de investigaţie imunoenzimatică omogenă şi trusă pentru realizarea lui

Info

Publication number
MD940033A
MD940033A MD94-0033A MD940033A MD940033A MD 940033 A MD940033 A MD 940033A MD 940033 A MD940033 A MD 940033A MD 940033 A MD940033 A MD 940033A
Authority
MD
Moldova
Prior art keywords
immunoassay
enzyme
enzymic
kit
carrying
Prior art date
Application number
MD94-0033A
Other languages
English (en)
Russian (ru)
Inventor
Franco Celada
Gyorgyo Gorog
Original Assignee
Applied Research Systems Ars Holding Nv (Nl/Na)
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Applied Research Systems Ars Holding Nv (Nl/Na) filed Critical Applied Research Systems Ars Holding Nv (Nl/Na)
Publication of MD940033A publication Critical patent/MD940033A/ro

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/577—Immunoassay; Biospecific binding assay; Materials therefor involving monoclonal antibodies binding reaction mechanisms characterised by the use of monoclonal antibodies; monoclonal antibodies per se are classified with their corresponding antigens
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/581—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with enzyme label (including co-enzymes, co-factors, enzyme inhibitors or substrates)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/967—Standards, controls, materials, e.g. validation studies, buffer systems
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/972—Modified antibody, e.g. hybrid, bifunctional
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/975—Kit

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Food Science & Technology (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Steroid Compounds (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Invenţia se referă la procedeul de investigaţie imunoenzimatică omogenă şi la trusa pentru realizarea lui. Procedeul prevede investigarea imunologică a ligandei în proba lichidă şi include: obţinerea amestecului din mostra lichidă, ensima labilă şi doi anticorpi monoclonali biospecifici diferiţi, formând imunocomplexul cuaternar; acţiunea asupra enzimei, ea devenind în consecinţă inactivă, şi determinarea enzimei, parţial cuplate în complex cu cantitatea de ligandă din mostră.
MD94-0033A 1988-12-23 1993-12-15 Procedeu de investigaţie imunoenzimatică omogenă şi trusă pentru realizarea lui MD940033A (ro)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
IT8848709A IT1235349B (it) 1988-12-23 1988-12-23 Saggio immunologico per determinazioni in fase omogenea
PCT/EP1989/001612 WO1990007714A1 (en) 1988-12-23 1989-12-22 Homogeneous encym immunoassay

Publications (1)

Publication Number Publication Date
MD940033A true MD940033A (ro) 1995-06-30

Family

ID=11268172

Family Applications (1)

Application Number Title Priority Date Filing Date
MD94-0033A MD940033A (ro) 1988-12-23 1993-12-15 Procedeu de investigaţie imunoenzimatică omogenă şi trusă pentru realizarea lui

Country Status (13)

Country Link
US (1) US5415998A (ro)
EP (1) EP0449863B1 (ro)
JP (1) JPH04505210A (ro)
AU (1) AU636593B2 (ro)
DE (1) DE68910134T2 (ro)
DK (1) DK113291D0 (ro)
ES (1) ES2060137T3 (ro)
IL (1) IL92861A0 (ro)
IT (1) IT1235349B (ro)
LT (1) LTIP1407A (ro)
LV (1) LV10738A (ro)
MD (1) MD940033A (ro)
WO (1) WO1990007714A1 (ro)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE193600T1 (de) * 1990-11-20 2000-06-15 Dade Behring Marburg Gmbh Verfahren zur stabilisierung von enzym-konjugaten
AU665758B2 (en) * 1991-04-26 1996-01-18 Surface Active Limited Novel antibodies, and methods for their use
TW205096B (ro) * 1991-05-29 1993-05-01 Shionogi & Co
US5447846A (en) * 1992-07-17 1995-09-05 Fuji Photo Film C., Ltd. Homogeneous Immunoassay process using covalent conjugate of enzyme and plural monoclonal antibodies for different epitopes on analyte
GB9316369D0 (en) * 1993-08-06 1993-09-22 Surface Active Ltd Diagnostic method
US5916757A (en) * 1994-05-31 1999-06-29 Johnson & Johnson Clinical Diagnostics, Inc. Specific binding assay using enzyme inhibitor and anti-inhibitor antibodies
US5565326A (en) * 1994-05-31 1996-10-15 Johnson & Johnson Clinical Diagnostics, Inc. Separation-free specific binding assays using anti-inhibitor antibodies
JP2000214165A (ja) * 1999-01-22 2000-08-04 Fuji Photo Film Co Ltd 均一系酵素免疫分析方法
US20030109067A1 (en) 2001-12-06 2003-06-12 Immunetech, Inc. Homogeneous immunoassays for multiple allergens

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4401764A (en) * 1980-02-07 1983-08-30 Technicon Instruments Corporation Immunoassays employing labeled reagent and a conjugate having two binding sites
JPS58122459A (ja) * 1982-01-14 1983-07-21 Yatoron:Kk 酵素の会合を利用した測定方法
ES521370A0 (es) * 1982-04-12 1985-04-16 Hybritech Inc Un procedimiento para obtener un polidoma.
CA1194415A (en) * 1982-05-05 1985-10-01 E. I. Du Pont De Nemours And Company Immunoglobulin half-molecules and process for producing hybrid antibodies
EP0119767B1 (en) * 1983-03-11 1990-11-22 FUJIREBIO KABUSHIKI KAISHA also trading as FUJIREBIO INC. Method of measuring ligands
US4687735A (en) * 1983-03-14 1987-08-18 Syntex (U.S.A.) Inc. Enzymatic poly-reactant channeling binding assay
DE3583278D1 (de) * 1984-04-23 1991-07-25 Boston Biomed Res Inst Doppelspezifische antikoerper-determinanten.
NL8501219A (nl) * 1985-04-29 1986-11-17 Stichting Vrienden Van De Stic Immunologisch complex, de bereiding en toepassing daarvan.
DE3600571A1 (de) * 1986-01-10 1987-08-06 Gruenenthal Gmbh Dna-sequenzen, die fuer proteine mit der biologischen aktivitaet der husi-typi-inhibitoren codieren, gentechnologische verfahren zur herstellung dieser proteine und diese proteine enthaltende arzneimittel
EP0310361A3 (en) * 1987-09-30 1989-05-24 Beckman Instruments, Inc. Tridentate conjugate and method of use thereof

Also Published As

Publication number Publication date
IT1235349B (it) 1992-06-30
JPH04505210A (ja) 1992-09-10
EP0449863B1 (en) 1993-10-20
DK113291A (da) 1991-06-13
IT8848709A0 (it) 1988-12-23
US5415998A (en) 1995-05-16
DE68910134T2 (de) 1994-04-07
WO1990007714A1 (en) 1990-07-12
AU636593B2 (en) 1993-05-06
IL92861A0 (en) 1990-09-17
EP0449863A1 (en) 1991-10-09
ES2060137T3 (es) 1994-11-16
DE68910134D1 (de) 1993-11-25
AU4745890A (en) 1990-08-01
LV10738A (lv) 1995-06-20
DK113291D0 (da) 1991-06-13
LTIP1407A (en) 1995-05-25

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Legal Events

Date Code Title Description
PD99 Pending application