MD847Y - Process for producing leafroll virus-free grapevine plants - Google Patents
Process for producing leafroll virus-free grapevine plants Download PDFInfo
- Publication number
- MD847Y MD847Y MDS20140052A MDS20140052A MD847Y MD 847 Y MD847 Y MD 847Y MD S20140052 A MDS20140052 A MD S20140052A MD S20140052 A MDS20140052 A MD S20140052A MD 847 Y MD847 Y MD 847Y
- Authority
- MD
- Moldova
- Prior art keywords
- free
- producing
- grapevine
- virus
- days
- Prior art date
Links
- 240000006365 Vitis vinifera Species 0.000 title abstract 3
- 238000000034 method Methods 0.000 title abstract 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 abstract 2
- 210000002257 embryonic structure Anatomy 0.000 abstract 2
- 235000015097 nutrients Nutrition 0.000 abstract 2
- 239000005708 Sodium hypochlorite Substances 0.000 abstract 1
- 241000219094 Vitaceae Species 0.000 abstract 1
- 235000014787 Vitis vinifera Nutrition 0.000 abstract 1
- 235000019441 ethanol Nutrition 0.000 abstract 1
- 235000021021 grapes Nutrition 0.000 abstract 1
- 238000005286 illumination Methods 0.000 abstract 1
- 238000000338 in vitro Methods 0.000 abstract 1
- 238000011081 inoculation Methods 0.000 abstract 1
- 239000003415 peat Substances 0.000 abstract 1
- 230000027665 photoperiodism Effects 0.000 abstract 1
- 230000010152 pollination Effects 0.000 abstract 1
- 238000000926 separation method Methods 0.000 abstract 1
- SUKJFIGYRHOWBL-UHFFFAOYSA-N sodium hypochlorite Chemical compound [Na+].Cl[O-] SUKJFIGYRHOWBL-UHFFFAOYSA-N 0.000 abstract 1
- 239000002689 soil Substances 0.000 abstract 1
- 230000001954 sterilising effect Effects 0.000 abstract 1
- 238000004659 sterilization and disinfection Methods 0.000 abstract 1
- 239000000758 substrate Substances 0.000 abstract 1
- 230000003612 virological effect Effects 0.000 abstract 1
Landscapes
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention relates to biotechnology and can be used in agriculture for producing leafroll virus-free grapevine plants.The process, according to the invention, comprises in vitro cultivation of immature embryos produced from stoneless grapevine parent forms and hybrid combinations thereof, vintage of bunches of grapes in 30…45 days after pollination, treatment at 4°C, sterilization with ethyl alcohol and sodium hypochlorite solution, separation of immature seeds and inoculation into a nutrient medium, cultivation in the dark at 25±2°C for 30 days, then at the same temperature, with a photoperiodism of 16 hours light, 8 hours darkness and illumination of 2000 lx, then the viable embryos are transferred to a nutrient medium, the formed plantlets are subjected to a virological control and the virus-free plantlets are transferred to soil and peat substrate.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| MDS20140052A MD847Z (en) | 2014-04-18 | 2014-04-18 | Process for producing leafroll virus-free grapevine plants |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| MDS20140052A MD847Z (en) | 2014-04-18 | 2014-04-18 | Process for producing leafroll virus-free grapevine plants |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| MD847Y true MD847Y (en) | 2014-12-31 |
| MD847Z MD847Z (en) | 2015-07-31 |
Family
ID=52211081
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MDS20140052A MD847Z (en) | 2014-04-18 | 2014-04-18 | Process for producing leafroll virus-free grapevine plants |
Country Status (1)
| Country | Link |
|---|---|
| MD (1) | MD847Z (en) |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| SU1682388A1 (en) * | 1988-08-02 | 1991-10-07 | Украинский научно-исследовательский институт сельскохозяйственной микробиологии | Method for cultivation of virus-free potatoes |
| UA19847U (en) * | 2006-06-06 | 2007-01-15 | Mechnikov Odesa Nat University | Workflow of revealing grapevine leafroll virus of first serotype |
| UA97981C2 (en) * | 2009-11-26 | 2012-04-10 | Інститут Сільськогосподарської Мікробіології Та Агропромислового Виробництва Національної Академії Аграрних Наук України | Method for cultivation of leaf-rolling mosaic virus in subinoculated lines of mammalian cell cultures |
-
2014
- 2014-04-18 MD MDS20140052A patent/MD847Z/en not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| MD847Z (en) | 2015-07-31 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FG9Y | Short term patent issued | ||
| KA4A | Patent for invention lapsed due to non-payment of fees (with right of restoration) |