MD819Z - Process for treating winter wheat against Fusarium oxysporum - Google Patents
Process for treating winter wheat against Fusarium oxysporum Download PDFInfo
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- MD819Z MD819Z MDS20140029A MDS20140029A MD819Z MD 819 Z MD819 Z MD 819Z MD S20140029 A MDS20140029 A MD S20140029A MD S20140029 A MDS20140029 A MD S20140029A MD 819 Z MD819 Z MD 819Z
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- oxysporum
- winter wheat
- fusarium oxysporum
- against fusarium
- treating
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- 241000223221 Fusarium oxysporum Species 0.000 title claims abstract description 27
- 241000209140 Triticum Species 0.000 title claims abstract description 16
- 238000000034 method Methods 0.000 title claims abstract description 14
- 235000021307 Triticum Nutrition 0.000 title claims abstract description 13
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims abstract description 15
- 241000983759 Linaria genistifolia Species 0.000 claims abstract description 8
- 229930182489 iridoid glycoside Natural products 0.000 claims abstract description 8
- 150000008145 iridoid glycosides Chemical class 0.000 claims abstract description 8
- 239000000284 extract Substances 0.000 claims abstract description 4
- 238000000605 extraction Methods 0.000 claims abstract description 3
- 241000196324 Embryophyta Species 0.000 claims description 6
- 238000009331 sowing Methods 0.000 claims description 5
- 239000007864 aqueous solution Substances 0.000 claims description 4
- 238000002791 soaking Methods 0.000 claims description 2
- 229930182470 glycoside Natural products 0.000 description 20
- 150000002338 glycosides Chemical class 0.000 description 19
- 235000013339 cereals Nutrition 0.000 description 9
- 230000035784 germination Effects 0.000 description 8
- 239000000243 solution Substances 0.000 description 7
- 244000250129 Trigonella foenum graecum Species 0.000 description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 5
- 239000000706 filtrate Substances 0.000 description 4
- 230000002786 root growth Effects 0.000 description 3
- 239000013543 active substance Substances 0.000 description 2
- 239000012153 distilled water Substances 0.000 description 2
- 239000002609 medium Substances 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 239000001965 potato dextrose agar Substances 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 238000005292 vacuum distillation Methods 0.000 description 2
- 238000001644 13C nuclear magnetic resonance spectroscopy Methods 0.000 description 1
- 238000005160 1H NMR spectroscopy Methods 0.000 description 1
- 206010017533 Fungal infection Diseases 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- DRWIVSAUXCDFQK-UHFFFAOYSA-N Linarioside Natural products C12C(C)(O)C(Cl)C(O)C2(O)C=COC1OC1OC(CO)C(O)C(O)C1O DRWIVSAUXCDFQK-UHFFFAOYSA-N 0.000 description 1
- 208000031888 Mycoses Diseases 0.000 description 1
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 1
- 235000001484 Trigonella foenum graecum Nutrition 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 229940088623 biologically active substance Drugs 0.000 description 1
- 238000009835 boiling Methods 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 230000002538 fungal effect Effects 0.000 description 1
- 230000000855 fungicidal effect Effects 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- XELZGAJCZANUQH-UHFFFAOYSA-N methyl 1-acetylthieno[3,2-c]pyrazole-5-carboxylate Chemical compound CC(=O)N1N=CC2=C1C=C(C(=O)OC)S2 XELZGAJCZANUQH-UHFFFAOYSA-N 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 230000007226 seed germination Effects 0.000 description 1
- 239000000741 silica gel Substances 0.000 description 1
- 229910002027 silica gel Inorganic materials 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 230000002269 spontaneous effect Effects 0.000 description 1
- 229930002534 steroid glycoside Natural products 0.000 description 1
- 150000008143 steroidal glycosides Chemical class 0.000 description 1
- 208000024891 symptom Diseases 0.000 description 1
- 238000004809 thin layer chromatography Methods 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 239000001917 trigonella foenum graecum l. absolute Substances 0.000 description 1
Landscapes
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
Invenţia se referă la agricultură, şi anume la un procedeu de tratare a grâului de toamnă contra Fusarium oxysporum.Procedeul, conform invenţiei, prevede înmuierea seminţelor de grâu înainte de semănat în decurs de 4 ore în soluţie apoasă de 10-5...10-3% de extract sumar de glicozide iridoidice din plantele Linaria genistifolia L. Mill, obţinut prin extragere cu metanol.The invention relates to agriculture, namely to a process for treating the autumn wheat against Fusarium oxysporum. -3% of summary extract of iridoid glycosides from plants Linaria genistifolia L. Mill, obtained by extraction with methanol.
Description
Invenţia se referă la agricultură, şi anume la un procedeu de tratare a grâului de toamnă contra Fusarium oxysporum. The invention relates to agriculture, namely to a process for treating winter wheat against Fusarium oxysporum.
Soluţia cea mai apropiată de procedeul revendicat constă în tratarea boabelor de grâu cu soluţii apoase de glicozide steroidice sumare (GSS) din Trigonella foenum-graecum L. [1]. The solution closest to the claimed process consists in treating wheat grains with aqueous solutions of total steroidal glycosides (GSS) from Trigonella foenum-graecum L. [1].
Eficacitatea acestui procedeu este relativ joasă. The effectiveness of this process is relatively low.
Dezavantajul acestui procedeu constă în faptul că GSS din T. foenum-graecum se obţin doar din seminţe, din care motiv randamentul de obţinere a acestora este destul de mic. În afară de aceasta, plantele de T. foenum-graecum nu au o răspândire largă, astfel accesibilitatea materiei prime fiind destul de limitată. The disadvantage of this process is that GSS from T. foenum-graecum can only be obtained from seeds, which is why the yield is quite low. In addition, T. foenum-graecum plants are not widely distributed, so the accessibility of the raw material is quite limited.
Problema pe care o rezolvă invenţia constă în sporirea rezistenţei plantelor de grâu de toamnă la F. oxysporum şi lărgirea sortimentului de substanţe biologic active naturale, cu acţiune fungicidă, inofensive pentru mediul ambiant şi organismele vii. The problem solved by the invention consists in increasing the resistance of winter wheat plants to F. oxysporum and expanding the range of natural biologically active substances with fungicidal action, harmless to the environment and living organisms.
Se propune un procedeu de sporire a rezistenţei plantelor de grâu de toamnă la F. oxysporum prin tratarea boabelor înainte de semănat cu substanţe biologic active pentru stimularea germinaţiei şi a creşterii rădăcinilor, totodată în calitate de substanţă biologic activă de origine naturală se utilizează glicozidele iridoidice sumare (GIS) din planta Linaria genistifolia L. Mill. A process is proposed to increase the resistance of winter wheat plants to F. oxysporum by treating the grains before sowing with biologically active substances to stimulate germination and root growth, while using the total iridoid glycosides (GIS) from the plant Linaria genistifolia L. Mill. as a biologically active substance of natural origin.
Procedeul de tratare a grâului de toamnă contra Fusarium oxysporum prevede înmuierea seminţelor de grâu înainte de semănat în decurs de 4 ore în soluţie apoasă de 10-5...10-3% de extract sumar de glicozide iridoidice din plantele Linaria genistifolia L. Mill, obţinut prin extragere cu metanol. The process of treating winter wheat against Fusarium oxysporum involves soaking wheat seeds before sowing for 4 hours in an aqueous solution of 10-5...10-3% of a summary extract of iridoid glycosides from Linaria genistifolia L. Mill plants, obtained by extraction with methanol.
Rezultatul invenţiei constă în sporea rezistenţei grâului de toamnă la putregaiul de rădăcină prin tratarea seminţelor de grâu înainte de semănat. The result of the invention consists in increasing the resistance of winter wheat to root rot by treating wheat seeds before sowing.
Procedeul contribuie la sporirea nivelului de germinaţie a boabelor şi la majorarea lungimii rădăciniţei embrionare cu 35,0% şi, respectiv, 30,4%, comparativ cu varianta cu filtratul de cultură (FC) F. oxysporum. The process contributes to increasing the level of grain germination and increasing the length of the embryonic root by 35.0% and, respectively, 30.4%, compared to the variant with the F. oxysporum culture filtrate (FC).
Metodă de obţinere a glicozidelor iridoidice Method for obtaining iridoid glycosides
Glicozidele iridoidice sumare din L. genistifolia s-au obţinut în modul următor: masa vegetală de plantă (partea aeriană) uscată, în cantitate de 300 g, a fost mărunţită şi extrasă cu soluţie hidrometanolică de 60% de 3 ori prin fierbere. Extractele au fost unite, concentrate prin distilare în vid, după care reziduul apos s-a trecut prin coloană cu Sephadex LH-20. Coloana a fost eluată cu apă, apoi cu soluţie hidrometanolică de 10%, după care cu metanol. The total iridoid glycosides from L. genistifolia were obtained as follows: the dried plant mass (aerial part), in an amount of 300 g, was ground and extracted with 60% hydromethanolic solution 3 times by boiling. The extracts were combined, concentrated by vacuum distillation, after which the aqueous residue was passed through a Sephadex LH-20 column. The column was eluted with water, then with 10% hydromethanolic solution, and then with methanol.
Eluatele cu hidrometanol de 10% şi metanol au fost unite şi concentrate prin distilare în vid până la uscat. În reziduul uscat (4,6 g) au fost detectate cu ajutorul cromatografiei în strat subţire de silicagel 4 glicozide iridoidice, denumite genistifoliozide 1-4. The 10% hydromethanol and methanol eluates were combined and concentrated by vacuum distillation to dryness. In the dry residue (4.6 g) 4 iridoid glycosides, named genistifoliosides 1-4, were detected by thin layer chromatography on silica gel.
Conform cercetărilor ulterioare, după separarea componentelor amestecului prin metode cromatografice, cu ajutorul procedeelor fizico-chimice, spectroscopiei H1 şi 13C RMN, genistifoliozidele 1-4 au fost identificate prin compararea cu datele bibliografice după cum urmează: 1 - antirinozida; 2 - linariozida; 3 - 5-O-alozilantirinozida; 4 - 6-β-hidroxiantirida. According to further research, after separating the components of the mixture by chromatographic methods, using physicochemical procedures, H1 and 13C NMR spectroscopy, genistifoliosides 1-4 were identified by comparison with bibliographic data as follows: 1 - antirhinoside; 2 - linarioside; 3 - 5-O-allosylantirhinoside; 4 - 6-β-hydroxyantirhinoside.
De asemenea, s-a stabilit că 5-O-alozilantirinozida este componenta majoră în amestecul sumar de glicozide iridoidice. It was also established that 5-O-allosylantirinoside is the major component in the summary mixture of iridoid glycosides.
Exemplu de realizare a invenţiei Example of embodiment of the invention
Obiectul de studiu a fost soiul de grâu comun de toamnă Odeschi 51. The object of study was the common winter wheat variety Odeschi 51.
Înainte de semănat, boabele au fost înmuiate timp de 4 ore în soluţii apoase de GIS din L. genistifolia (invenţia propusă) şi de GSS din T. foenum-graecum (cea mai apropiată soluţie) în concentraţiile 10-5, 10-4, 10-3 , 10-2 %. Ca variantă martor au servit boabele înmuiate în apă. După uscare timp de 24 ore, boabele au fost înmuiate timp de 18 ore în filtratul de cultură (FC) F. oxysporum de 21 zile, după care s-au clătit de 2 ori cu apă distilată. FC F. oxysporum a fost obţinut în modul următor: tulpina de fungi F. oxysporum a fost izolată din baza tulpinii de grâu comun de toamnă cu simptome de boală (pete brunificate) pe mediul nutritiv solid Potato Dextrose Agar (PDA), identificate prin analize macro- şi microscopice. Filtratul de cultură al ciupercii F. oxysporum a fost obţinut prin inocularea miceliului în mediul nutritiv Cszapek şi cultivarea ulterioară la temperatura de 22…24ºC, timp de 21 zile [Методы экспериментальной микологии. Киев, Наукова думка, 1982, p. 550]. Before sowing, the grains were soaked for 4 hours in aqueous solutions of GIS from L. genistifolia (the proposed invention) and GSS from T. foenum-graecum (the closest solution) in concentrations of 10-5, 10-4, 10-3 , 10-2 %. Grains soaked in water served as a control. After drying for 24 hours, the grains were soaked for 18 hours in the 21-day culture filtrate (FC) of F. oxysporum, after which they were rinsed 2 times with distilled water. FC F. oxysporum was obtained as follows: the F. oxysporum fungal strain was isolated from the base of the common winter wheat stem with disease symptoms (brown spots) on the solid nutrient medium Potato Dextrose Agar (PDA), identified by macro- and microscopic analyses. The culture filtrate of the fungus F. oxysporum was obtained by inoculating the mycelium in the Cszapek nutrient medium and subsequent cultivation at a temperature of 22…24ºC for 21 days [Methods of experimental mycology. Kiev, Naukova Dumka, 1982, p. 550].
Boabele tratate au fost plasate în cutii Petri pe hârtie de filtru umectată cu apă distilată la 22°C pentru 6 zile. The treated grains were placed in Petri dishes on filter paper moistened with distilled water at 22°C for 6 days.
Reacţia plantelor a fost apreciată în baza capacităţii germinative şi după lungimea rădăciniţei embrionare. Experienţa s-a efectuat în 3 repetiţii. Datele au fost prelucrate în programul STATISTICA 7. The reaction of the plants was assessed based on the germination capacity and the length of the embryonic root. The experiment was carried out in 3 repetitions. The data were processed in the STATISTICA 7 program.
Rezultatele obţinute denotă că infectarea boabelor cu FC F. oxysporum diminuează facultatea germinativă a seminţelor cu 33,4% şi creşterea rădăciniţei embrionare cu 35,7% comparativ cu varianta martor (tabelul 1). Aceasta demonstrează toxicitatea ciupercii care provoacă putregaiul de rădăcină. The results obtained show that infection of grains with FC F. oxysporum reduces seed germination by 33.4% and embryonic root growth by 35.7% compared to the control (Table 1). This demonstrates the toxicity of the fungus that causes root rot.
În cazul seminţelor tratate cu GSS în concentraţiile 10-5 ... 10-2 %, plantele au prezentat un nivel de germinaţie de 76,7 ...80,0% şi o creştere a lungimii rădăciniţei de 51,46 ... 58,69 mm, iar cu soluţii de GIS - de 86,7... 96,7% şi 55,67...59,59 mm, respectiv. Pentru ambele glicozide sumare, cele mai eficiente concentraţii au fost 10-4 şi 10-3%, în cazul cărora GSS, comparativ cu varianta FC F.oxysporum, au prezentat pentru germinaţie şi lungimea rădăciniţei un efect de +16,7…+23,4% şi, respectiv, +13,1 … +21,1%, iar soluţiile de GIS: +35,0% şi, respectiv, +23,4…+30,4%. In the case of seeds treated with GSS in concentrations of 10-5 ... 10-2 %, the plants showed a germination level of 76.7 ... 80.0% and an increase in root length of 51.46 ... 58.69 mm, and with GIS solutions - of 86.7 ... 96.7% and 55.67 ... 59.59 mm, respectively. For both summary glycosides, the most effective concentrations were 10-4 and 10-3%, in which GSS, compared to the FC F.oxysporum variant, showed an effect of +16.7 ... +23.4% and, respectively, +13.1 ... +21.1% for germination and root length, and GIS solutions: +35.0% and, respectively, +23.4 ... +30.4%.
Influenţa genistifoliozidelor iridoidice sumare asupra reacţiei plantelor de grâu la filtratul de cultură F. oxysporum Influence of summary genistifoliosides iridoides on the reaction of wheat plants to F. oxysporum culture filtrate
Tabelul 1 Table 1
Nr. Varianta/concentraţia, % Germinaţia, % Lungimea rădăciniţei embrionare, mm % în raport cu FC F.oxysporum 1 Martor (H2O) 96,7 80,81±1,80 155,49 2 FC F. oxysporum 63,3 51,97±2,57 - 3 FC F. oxysporum + GSS, 10-2 80,0 58,69±2,03* 112,94 4 FC F. oxysporum + GSS, 10-3 86,7 62,94±1,86* 121,11 5 FC F. oxysporum + GSS, 10-4 80,0 58,77±1,02* 113,09 6 FC F. oxysporum + GSS, 10-5 76,7 51,46±1,46 99,02 7 FC F. oxysporum + GIS, 10-2 96,7 59,59±1,82* 114,66 8 FC F. oxysporum + GIS, 10-3 98,3 67,75±1,63* 130,36 9 FC F. oxysporum + GIS, 10-4 98,3 64,15±1,59* 123,44 10 FC F. oxysporum + GIS, 10-5 86,7 55,67±0,94 107,12No. Variant/concentration, % Germination, % Length of embryonic root, mm % in relation to FC F.oxysporum 1 Control (H2O) 96.7 80.81±1.80 155.49 2 FC F. oxysporum 63.3 51.97±2.57 - 3 FC F. oxysporum + GSS, 10-2 80.0 58.69±2.03* 112.94 4 FC F. oxysporum + GSS, 10-3 86.7 62.94±1.86* 121.11 5 FC F. oxysporum + GSS, 10-4 80.0 58.77±1.02* 113.09 6 FC F. oxysporum + GSS, 10-5 76.7 51.46±1.46 99.02 7 FC F. oxysporum + GIS, 10-2 96.7 59.59±1.82* 114.66 8 FC F. oxysporum + GIS, 10-3 98.3 67.75±1.63* 130.36 9 FC F. oxysporum + GIS, 10-4 98.3 64.15±1.59* 123.44 10 FC F. oxysporum + GIS, 10-5 86.7 55.67±0.94 107.12
*- veridic în raport cu FC F. oxysporum la p≤0,05. *- true in relation to FC F. oxysporum at p≤0.05.
Astfel, tratarea boabelor cu GIS din Linaria genistifolia L. are o eficienţă mai înaltă în ceea ce priveşte capacitatea de germinaţie şi creşterea rădăciniţei embrionare, în comparaţie cu soluţia apropiată - GSS din T. foenum-graecum, la infectarea cu F.oxysporum. Thus, treating grains with GIS from Linaria genistifolia L. has a higher efficiency in terms of germination capacity and embryonic root growth, compared to the close solution - GSS from T. foenum-graecum, when infected with F.oxysporum.
Prin urmare, procedeul elaborat este tehnologic simplu, eficient şi permite utilizarea substanţelor naturale, ecologic inofensive, şi anume a genistifoliozidelor, obţinute din planta Linaria genistifolia L. Mill care creşte în flora spontană şi poate fi colectată în cantităţi mari, în agricultură în calitate de substanţe protectoare, în cazul infecţiilor fungice care afectează germinaţia, creşterea şi dezvoltarea rădăcinii de grâu. Therefore, the developed process is technologically simple, efficient and allows the use of natural, ecologically harmless substances, namely genistifoliosides, obtained from the Linaria genistifolia L. Mill plant which grows in the spontaneous flora and can be collected in large quantities, in agriculture as protective substances, in the case of fungal infections that affect the germination, growth and development of wheat roots.
1. MD 2716 G2 2005.03.31 1. MD 2716 G2 2005.03.31
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| Application Number | Priority Date | Filing Date | Title |
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| MDS20140029A MD819Z (en) | 2014-03-07 | 2014-03-07 | Process for treating winter wheat against Fusarium oxysporum |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| MD1237Z (en) * | 2017-08-10 | 2018-10-31 | Институт Микробиологии И Биотехнологии Академии Наук Молдовы | Process for presowing treatment of cereal seeds |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2069940C1 (en) * | 1992-07-17 | 1996-12-10 | Институт генетики АН Республика Молдова | Method of presowing wheat seeds treatment |
| MD2037G2 (en) * | 2001-04-26 | 2003-05-31 | Институт Генетики И Физиологии Растений Академии Наук Молдовы | Process for obtaining the 22-O-hydroxyfurostanols |
| MD2716G2 (en) * | 2004-04-13 | 2005-10-31 | Институт Генетики И Физиологии Растений Академии Наук Молдовы | Antistressor for winter common wheat |
| MD3866C2 (en) * | 2008-12-19 | 2009-10-31 | Институт Генетики, Физиологии И Защиты Растений Академии Наук Молдовы | 5,4-dimethylcampherol 3-O--D-(6′′-a′-L-rhamnopyranosyl)-glucopyranoside as compound increasing the seed productivity |
| MD365Z (en) * | 2011-01-10 | 2011-12-31 | Институт Генетики, Физиологии И Защиты Растений Академии Наук Молдовы | Process for presowing treatment of carrot seeds |
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2014
- 2014-03-07 MD MDS20140029A patent/MD819Z/en not_active IP Right Cessation
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2069940C1 (en) * | 1992-07-17 | 1996-12-10 | Институт генетики АН Республика Молдова | Method of presowing wheat seeds treatment |
| MD2037G2 (en) * | 2001-04-26 | 2003-05-31 | Институт Генетики И Физиологии Растений Академии Наук Молдовы | Process for obtaining the 22-O-hydroxyfurostanols |
| MD2716G2 (en) * | 2004-04-13 | 2005-10-31 | Институт Генетики И Физиологии Растений Академии Наук Молдовы | Antistressor for winter common wheat |
| MD3866C2 (en) * | 2008-12-19 | 2009-10-31 | Институт Генетики, Физиологии И Защиты Растений Академии Наук Молдовы | 5,4-dimethylcampherol 3-O--D-(6′′-a′-L-rhamnopyranosyl)-glucopyranoside as compound increasing the seed productivity |
| MD365Z (en) * | 2011-01-10 | 2011-12-31 | Институт Генетики, Физиологии И Защиты Растений Академии Наук Молдовы | Process for presowing treatment of carrot seeds |
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