MD620C2 - Process for Bacillus cereus indication - Google Patents
Process for Bacillus cereus indicationInfo
- Publication number
- MD620C2 MD620C2 MD95-0209A MD950209A MD620C2 MD 620 C2 MD620 C2 MD 620C2 MD 950209 A MD950209 A MD 950209A MD 620 C2 MD620 C2 MD 620C2
- Authority
- MD
- Moldova
- Prior art keywords
- indication
- incubation
- bacillus cereus
- nutrient medium
- bisubstituted
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 4
- 241000193755 Bacillus cereus Species 0.000 title abstract 3
- 235000015097 nutrients Nutrition 0.000 abstract 5
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 abstract 4
- LWIHDJKSTIGBAC-UHFFFAOYSA-K tripotassium phosphate Chemical class [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 abstract 4
- 238000011534 incubation Methods 0.000 abstract 3
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 abstract 2
- 239000008103 glucose Substances 0.000 abstract 2
- 229910000160 potassium phosphate Inorganic materials 0.000 abstract 2
- 235000011009 potassium phosphates Nutrition 0.000 abstract 2
- 239000011780 sodium chloride Substances 0.000 abstract 2
- 241000894006 Bacteria Species 0.000 abstract 1
- 108010010803 Gelatin Proteins 0.000 abstract 1
- 229930006000 Sucrose Natural products 0.000 abstract 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 abstract 1
- 230000001133 acceleration Effects 0.000 abstract 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 abstract 1
- 235000010633 broth Nutrition 0.000 abstract 1
- 235000013681 dietary sucrose Nutrition 0.000 abstract 1
- 229920000159 gelatin Polymers 0.000 abstract 1
- 239000008273 gelatin Substances 0.000 abstract 1
- 235000019322 gelatine Nutrition 0.000 abstract 1
- 235000011852 gelatine desserts Nutrition 0.000 abstract 1
- 235000001727 glucose Nutrition 0.000 abstract 1
- 239000004615 ingredient Substances 0.000 abstract 1
- 239000000463 material Substances 0.000 abstract 1
- 239000000203 mixture Substances 0.000 abstract 1
- -1 monosubstituted sodium phosphate Chemical class 0.000 abstract 1
- 238000010899 nucleation Methods 0.000 abstract 1
- 235000002639 sodium chloride Nutrition 0.000 abstract 1
- 229960004793 sucrose Drugs 0.000 abstract 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract 1
Landscapes
- Saccharide Compounds (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention relates to the medical microbiology and may be used for express-indication of Bacillus cereus.Summary of the proposed invention consists in the fact that the process includes seeding of the investigated material onto the nutrient medium, further seedinos incubation and serching bacteria indication where the incubation is made onto the nutrient medium containing the dry nutrient broth, gelatin, sodium chloride, glucose, saccharose, bisubstituted potassium phosphate, monosubstituted sodium phosphate and water, at the fallowing ingredients quantity ratio, mg/l:bisubstituted potassium phosphate 8,875-9,50Dried at 45°C and sterilized in the ultraviolet rays during 2 hours, by that the incubation is conducted during 9 hours.The technical result of the invention consists in the process acceleration, owing to the use of dry nutrient medium in which the dry nutrient broth and the glucose alow to improve the Bacillus cereus grow and propagation as well as in increasing the process sensibility by the introduction of sodium chloride into the composition of the medium.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| MD95-0209A MD620C2 (en) | 1995-03-06 | 1995-03-06 | Process for Bacillus cereus indication |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| MD95-0209A MD620C2 (en) | 1995-03-06 | 1995-03-06 | Process for Bacillus cereus indication |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| MD620F1 MD620F1 (en) | 1996-11-29 |
| MD620C2 true MD620C2 (en) | 1997-04-30 |
Family
ID=19738655
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MD95-0209A MD620C2 (en) | 1995-03-06 | 1995-03-06 | Process for Bacillus cereus indication |
Country Status (1)
| Country | Link |
|---|---|
| MD (1) | MD620C2 (en) |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4444883A (en) * | 1982-08-09 | 1984-04-24 | E. R. Squibb & Sons, Inc. | Method for the isolation and presumptive diagnosis of Bacillus cereus and tellurite medium therefor |
-
1995
- 1995-03-06 MD MD95-0209A patent/MD620C2/en active IP Right Grant
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4444883A (en) * | 1982-08-09 | 1984-04-24 | E. R. Squibb & Sons, Inc. | Method for the isolation and presumptive diagnosis of Bacillus cereus and tellurite medium therefor |
Also Published As
| Publication number | Publication date |
|---|---|
| MD620F1 (en) | 1996-11-29 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FG3A | Granted patent for invention | ||
| IF99 | Valid patent on 19990615 |
Free format text: EXPIRES: 20150306 |