MD4828B1 - Process for submerged cultivation of Rhizopus arrhizus CNMN FD 03 fungus strain, producer of lipases - Google Patents
Process for submerged cultivation of Rhizopus arrhizus CNMN FD 03 fungus strain, producer of lipasesInfo
- Publication number
- MD4828B1 MD4828B1 MDA20210070A MD20210070A MD4828B1 MD 4828 B1 MD4828 B1 MD 4828B1 MD A20210070 A MDA20210070 A MD A20210070A MD 20210070 A MD20210070 A MD 20210070A MD 4828 B1 MD4828 B1 MD 4828B1
- Authority
- MD
- Moldova
- Prior art keywords
- cultivation
- cnmn
- lipases
- producer
- strain
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 3
- 241000233866 Fungi Species 0.000 title abstract 2
- 102000004882 Lipase Human genes 0.000 title abstract 2
- 108090001060 Lipase Proteins 0.000 title abstract 2
- 239000004367 Lipase Substances 0.000 title abstract 2
- 240000005384 Rhizopus oryzae Species 0.000 title abstract 2
- 235000013752 Rhizopus oryzae Nutrition 0.000 title abstract 2
- 235000019421 lipase Nutrition 0.000 title abstract 2
- 102000004190 Enzymes Human genes 0.000 abstract 2
- 108090000790 Enzymes Proteins 0.000 abstract 2
- 239000003814 drug Substances 0.000 abstract 2
- 230000002366 lipolytic effect Effects 0.000 abstract 2
- 239000000725 suspension Substances 0.000 abstract 2
- 229920001817 Agar Polymers 0.000 abstract 1
- 239000007836 KH2PO4 Substances 0.000 abstract 1
- 239000008272 agar Substances 0.000 abstract 1
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 abstract 1
- 229910052921 ammonium sulfate Inorganic materials 0.000 abstract 1
- 235000011130 ammonium sulphate Nutrition 0.000 abstract 1
- 239000012736 aqueous medium Substances 0.000 abstract 1
- 230000015572 biosynthetic process Effects 0.000 abstract 1
- 239000000032 diagnostic agent Substances 0.000 abstract 1
- 229940039227 diagnostic agent Drugs 0.000 abstract 1
- 239000003925 fat Substances 0.000 abstract 1
- 235000013312 flour Nutrition 0.000 abstract 1
- 235000013305 food Nutrition 0.000 abstract 1
- 238000011081 inoculation Methods 0.000 abstract 1
- 238000004519 manufacturing process Methods 0.000 abstract 1
- 239000002609 medium Substances 0.000 abstract 1
- 230000002906 microbiologic effect Effects 0.000 abstract 1
- 229910000402 monopotassium phosphate Inorganic materials 0.000 abstract 1
- 235000019796 monopotassium phosphate Nutrition 0.000 abstract 1
- 235000015097 nutrients Nutrition 0.000 abstract 1
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 abstract 1
- 238000002360 preparation method Methods 0.000 abstract 1
- 238000003756 stirring Methods 0.000 abstract 1
- 229940124597 therapeutic agent Drugs 0.000 abstract 1
- 230000001225 therapeutic effect Effects 0.000 abstract 1
- 235000015112 vegetable and seed oil Nutrition 0.000 abstract 1
- 239000008158 vegetable oil Substances 0.000 abstract 1
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention relates to biotechnology, and in particular to a process for submerged cultivation of Rhizopus arrhizus CNMN FD 03 fungus strain, producer of lipases and can be used in the microbiological industry for obtaining lipolytic enzymes with wide application in the food industry, production and processing of fats and vegetable oils, in medicine as a therapeutic and diagnostic agent.The process, according to the invention, provides for the preparation of a spore suspension of the strain grown for 30 days on a malt-agar medium, inoculation of the suspension in an amount of 5 vol.% in a nutrient aqueous medium containing, g/L: soy flour - 35.0, (NH4)2SO4 - 1.0, KH2PO4 - 5.0, with the simultaneous addition of 0.010 g/L of [Ca(L)3][Co(NCS)4], where L - dimethylpyridine-2,6-dicarboxylate, and cultivation with continuous stirring at 180-200 rpm at the temperature of 28-30°C for 24 hours.The result of the invention consists in increasing the biosynthesis of lipolytic enzymes and reducing the duration of cultivation of the strain by 24 hours.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| MDA20210070A MD4828C8 (en) | 2021-10-11 | 2021-10-11 | Process for submerged cultivation of Rhizopus arrhizus CNMN FD 03 fungus strain, producer of lipases |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| MDA20210070A MD4828C8 (en) | 2021-10-11 | 2021-10-11 | Process for submerged cultivation of Rhizopus arrhizus CNMN FD 03 fungus strain, producer of lipases |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| MD4828B1 true MD4828B1 (en) | 2022-10-31 |
| MD4828C1 MD4828C1 (en) | 2023-05-31 |
| MD4828C8 MD4828C8 (en) | 2024-01-31 |
Family
ID=83897939
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MDA20210070A MD4828C8 (en) | 2021-10-11 | 2021-10-11 | Process for submerged cultivation of Rhizopus arrhizus CNMN FD 03 fungus strain, producer of lipases |
Country Status (1)
| Country | Link |
|---|---|
| MD (1) | MD4828C8 (en) |
-
2021
- 2021-10-11 MD MDA20210070A patent/MD4828C8/en active IP Right Grant
Also Published As
| Publication number | Publication date |
|---|---|
| MD4828C8 (en) | 2024-01-31 |
| MD4828C1 (en) | 2023-05-31 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FG4A | Patent for invention issued | ||
| TC4A | Change of name of proprietor (patent for invention) |
Owner name: INSTITUTIA PUBLICA UNIVERSITATEA TEHNICA A MOLDOVEI, MD BD. STEFAN CEL MARE SI SFANT NR. 168, MD-2004, CHISINAU, REPUBLICA MOLDOVAINSTITUTUL DE CHIMIE, MD STR. ACADEMIEI NR. 3, MD-2028, REPUBLICA MOLDOVA Free format text: PREVIOUS NAME OF PROPRIETOR: INSTITUTUL DE MICROBIOLOGIE SI BIOTEHNOLOGIE, MD STR. ACADEMIEI NR. 1, MD-2028, CHISINAU, REPUBLICA MOLDOVAINSTITUTUL DE CHIMIE, MD STR. ACADEMIEI NR. 3, MD-2028, REPUBLICA MOLDOVA |