MD4205B1 - Metoda de determinare a activitatii catalazei - Google Patents

Metoda de determinare a activitatii catalazei

Info

Publication number
MD4205B1
MD4205B1 MD20120004A MD20120004A MD4205B1 MD 4205 B1 MD4205 B1 MD 4205B1 MD 20120004 A MD20120004 A MD 20120004A MD 20120004 A MD20120004 A MD 20120004A MD 4205 B1 MD4205 B1 MD 4205B1
Authority
MD
Moldova
Prior art keywords
catalase
activity
determining
phosphate buffer
mixture
Prior art date
Application number
MD20120004A
Other languages
English (en)
Moldavian (mo)
Other versions
MD4205C1 (ro
Inventor
Nadejda Efremova
Agafia Usatihj
Elena Molodoi
Agafia USATII
Original Assignee
Inst De Microbiologie Si Biotehnologie Al Academiei De Stiinte A Moldovei
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Inst De Microbiologie Si Biotehnologie Al Academiei De Stiinte A Moldovei filed Critical Inst De Microbiologie Si Biotehnologie Al Academiei De Stiinte A Moldovei
Priority to MDA20120004A priority Critical patent/MD4205C1/ro
Publication of MD4205B1 publication Critical patent/MD4205B1/ro
Publication of MD4205C1 publication Critical patent/MD4205C1/ro

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  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Inventia se refera la biotehnologie, în special la o metoda de determinare a activitatii catalazei si poate fi utilizata în cercetari biochimice, precum si pentru controlul procesului tehnologic de obtinere a preparatului catalazei în industria microbiologica.Esenta metodei consta în aceea ca materialul biologic se introduce într-un amestec ce contine: 0,05 M de tampon fosfat, 0,06% de peroxid de hidrogen si 5 mM de acid etilendiamintetraacetic, apoi amestecul se incubeaza la temperatura de 25ºC si se determina spectrofotometric densitatea optica a produsului reactiei în raport cu solutia de tampon fosfat, masurarile se efectueaza la lungimea de unda de 240 nm la începutul reactiei si peste 15 secunde, iar activitatea catalazei se determina conform unei formule stabilite.
MDA20120004A 2011-12-26 2011-12-26 Metodă de determinare a activităţii catalazei MD4205C1 (ro)

Priority Applications (1)

Application Number Priority Date Filing Date Title
MDA20120004A MD4205C1 (ro) 2011-12-26 2011-12-26 Metodă de determinare a activităţii catalazei

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
MDA20120004A MD4205C1 (ro) 2011-12-26 2011-12-26 Metodă de determinare a activităţii catalazei

Publications (2)

Publication Number Publication Date
MD4205B1 true MD4205B1 (ro) 2013-02-28
MD4205C1 MD4205C1 (ro) 2013-09-30

Family

ID=47831226

Family Applications (1)

Application Number Title Priority Date Filing Date
MDA20120004A MD4205C1 (ro) 2011-12-26 2011-12-26 Metodă de determinare a activităţii catalazei

Country Status (1)

Country Link
MD (1) MD4205C1 (ro)

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SU1399682A1 (ru) * 1985-07-02 1988-05-30 В.И. Дудин Способ определени каталазы в крови
SU1436070A1 (ru) * 1986-11-18 1988-11-07 Воронежский государственный университет им.Ленинского комсомола Способ определени каталазной активности почв
SU1422121A1 (ru) * 1987-01-21 1988-09-07 Институт Ботаники Ан Казсср Способ вы влени каталазной активности в биологических объектах
WO1990002202A1 (en) * 1988-08-16 1990-03-08 Cetus Corporation Reduction of peroxidatic and catalatic interference with assays of peroxidatic activity
RU2027171C1 (ru) * 1991-07-05 1995-01-20 Узбекский научно-исследовательский институт каракулеводства Способ определения активности каталазы в биологических объектах
GB9926908D0 (en) * 1999-11-12 2000-01-12 Smithkline Beecham Plc Novel process
RU2175445C2 (ru) * 1999-11-15 2001-10-27 Научно-исследовательский институт кардиологии Томского научного центра СО РАМН Среда инкубации для определения активности каталазы в плазме крови
RU2235320C1 (ru) * 2003-03-13 2004-08-27 Московский государственный университет леса Способ определения скорости разложения пероксида водорода каталазой в почвах
US7410755B2 (en) * 2005-02-22 2008-08-12 Discoverx ADP detection using an enzyme-coupled reaction
CN101464389A (zh) * 2007-12-19 2009-06-24 苏州艾杰生物科技有限公司 单胺氧化酶诊断试剂盒及单胺氧化酶活性浓度测定方法

Also Published As

Publication number Publication date
MD4205C1 (ro) 2013-09-30

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FG4A Patent for invention issued
KA4A Patent for invention lapsed due to non-payment of fees (with right of restoration)
MM4A Patent for invention definitely lapsed due to non-payment of fees