MD4054C1 - Method for determining the cobalt binding ability of the ischemic modified albumin - Google Patents

Method for determining the cobalt binding ability of the ischemic modified albumin

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Publication number
MD4054C1
MD4054C1 MDA20090116A MD20090116A MD4054C1 MD 4054 C1 MD4054 C1 MD 4054C1 MD A20090116 A MDA20090116 A MD A20090116A MD 20090116 A MD20090116 A MD 20090116A MD 4054 C1 MD4054 C1 MD 4054C1
Authority
MD
Moldova
Prior art keywords
binding ability
modified albumin
determining
cobalt binding
cobalt
Prior art date
Application number
MDA20090116A
Other languages
Romanian (ro)
Russian (ru)
Other versions
MD4054B1 (en
Inventor
Валентин ГУДУМАК
Ольга ТАГАДЮК
Original Assignee
Государственный Медицинский И Фармацевтический Университет "Nicolae Testemitanu" Республики Молдова
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Государственный Медицинский И Фармацевтический Университет "Nicolae Testemitanu" Республики Молдова filed Critical Государственный Медицинский И Фармацевтический Университет "Nicolae Testemitanu" Республики Молдова
Priority to MDA20090116A priority Critical patent/MD4054C1/en
Publication of MD4054B1 publication Critical patent/MD4054B1/en
Publication of MD4054C1 publication Critical patent/MD4054C1/en

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Abstract

The invention relates to medicine, in particular to a method for determining the cobalt binding ability of the ischemic modified albumin and can be used for the diagnosis of pathological processes caused by tissue and organ hypoxia, the study of their pathogenesis and the determination of treatment methods.The method, according to the invention, includes the treatment of biological material with 0.50...0.75 mM solution of cobalt chloride, mixing for 5 min at room temperature, addition of incubation medium, containing, mM: dithiothreitol 10.0...20.0, HEPES buffer solution pH 7.4 10.0...25.0, sodium chloride 140.0...150.0 and heptanol 0.3...1.0, stirring for 2 min, measurement of optical density of the obtained complex at a wavelength of 485 nm and determination of cobalt binding ability of the ischemic modified albumin.
MDA20090116A 2009-11-06 2009-11-06 Method for determining the cobalt binding ability of the ischemic modified albumin MD4054C1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
MDA20090116A MD4054C1 (en) 2009-11-06 2009-11-06 Method for determining the cobalt binding ability of the ischemic modified albumin

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
MDA20090116A MD4054C1 (en) 2009-11-06 2009-11-06 Method for determining the cobalt binding ability of the ischemic modified albumin

Publications (2)

Publication Number Publication Date
MD4054B1 MD4054B1 (en) 2010-07-30
MD4054C1 true MD4054C1 (en) 2011-02-28

Family

ID=43569012

Family Applications (1)

Application Number Title Priority Date Filing Date
MDA20090116A MD4054C1 (en) 2009-11-06 2009-11-06 Method for determining the cobalt binding ability of the ischemic modified albumin

Country Status (1)

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MD (1) MD4054C1 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102507916A (en) * 2011-11-07 2012-06-20 宁波美康生物科技股份有限公司 Ischemia modified albumin liquid stabilization kit

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0413678A2 (en) * 1989-08-17 1991-02-20 Manfred Dr. Kessler Method for the detection and determination of human serum albumin in biological fluids using fluorogenic cyanine dyes
SU1677632A1 (en) * 1989-09-10 1991-09-15 Азербайджанский Государственный Медицинский Институт Им.Н.Нариманова Method for examination of cardiovascular system
SU1720004A1 (en) * 1990-01-18 1992-03-15 Научно-Исследовательский Институт Физико-Химической Медицины Мз Рсфср Method for determining albumin-globulin blood coefficient
RU2102756C1 (en) * 1995-05-22 1998-01-20 Сибирский медицинский университет Method of prognosis of ischemic heart disease course
RU2182709C2 (en) * 2000-04-28 2002-05-20 Уральский НИИ охраны материнства и младенчества МЗ РФ Method or preclinical diagnosis of hepatodepressive syndrome in gestation
MD3394G2 (en) * 2007-01-09 2008-04-30 Диомид ГЕРМАН Method of spinal marrow infarction diagnosis

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0413678A2 (en) * 1989-08-17 1991-02-20 Manfred Dr. Kessler Method for the detection and determination of human serum albumin in biological fluids using fluorogenic cyanine dyes
SU1677632A1 (en) * 1989-09-10 1991-09-15 Азербайджанский Государственный Медицинский Институт Им.Н.Нариманова Method for examination of cardiovascular system
SU1720004A1 (en) * 1990-01-18 1992-03-15 Научно-Исследовательский Институт Физико-Химической Медицины Мз Рсфср Method for determining albumin-globulin blood coefficient
RU2102756C1 (en) * 1995-05-22 1998-01-20 Сибирский медицинский университет Method of prognosis of ischemic heart disease course
RU2182709C2 (en) * 2000-04-28 2002-05-20 Уральский НИИ охраны материнства и младенчества МЗ РФ Method or preclinical diagnosis of hepatodepressive syndrome in gestation
MD3394G2 (en) * 2007-01-09 2008-04-30 Диомид ГЕРМАН Method of spinal marrow infarction diagnosis

Non-Patent Citations (9)

* Cited by examiner, † Cited by third party
Title
Bhagavan N.V., Lai E.M., Rios P.A., et. al. Evaluation of Human Serum Albumin Cobalt Binding Assay for the Assesment of Myocardial Ischemia and *
Bhagavan N.V., Lai E.M., Rios P.A., et. al. Evaluation of Human Serum Albumin Cobalt Binding Assay for the Assesment of Myocardial Ischemia and Myocardial Infarction. Clin. Chemistry, 2003, 49:4, p. 581-585 *
Christenson R.H., Hong Duh Sh., Sanhal W.R., et. al. Caracteristics of an Albumin Cobalt Binding Test for Assesment of Acute Coronary Syndrome *
Christenson R.H., Hong Duh Sh., Sanhal W.R., et. al. Caracteristics of an Albumin Cobalt Binding Test for Assesment of Acute Coronary Syndrome Patients: A Multicenter Study. Clin. Chemistry, 2001, 47:3, p. 464-470 *
Myocardial Infarction. Clin. Chemistry, 2003, 49:4, p. 581-585. *
Patients: A Multicenter Study. Clin. Chemistry, 2001, 47:3, p. 464-470. *
Литус (Верхоланцева)Е.А. Влияние химической модификации, вызываемой действием окислителей, на Со - связывающую активность *
Литус Е.А., Гаврилова О.Д.. Со-связующая способность нативного и обработанного гипохлорит-анионом сывороточного альбумина. Вестник *
сывороточного альбумина. www.volgmen.ru/news/2007.06.07.a.php. *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102507916A (en) * 2011-11-07 2012-06-20 宁波美康生物科技股份有限公司 Ischemia modified albumin liquid stabilization kit
CN102507916B (en) * 2011-11-07 2013-11-06 宁波美康生物科技股份有限公司 Ischemia modified albumin liquid stabilization kit

Also Published As

Publication number Publication date
MD4054B1 (en) 2010-07-30

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FG4A Patent for invention issued
KA4A Patent for invention lapsed due to non-payment of fees (with right of restoration)