MD4054C1 - Method for determining the cobalt binding ability of the ischemic modified albumin - Google Patents
Method for determining the cobalt binding ability of the ischemic modified albuminInfo
- Publication number
- MD4054C1 MD4054C1 MDA20090116A MD20090116A MD4054C1 MD 4054 C1 MD4054 C1 MD 4054C1 MD A20090116 A MDA20090116 A MD A20090116A MD 20090116 A MD20090116 A MD 20090116A MD 4054 C1 MD4054 C1 MD 4054C1
- Authority
- MD
- Moldova
- Prior art keywords
- binding ability
- modified albumin
- determining
- cobalt binding
- cobalt
- Prior art date
Links
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- Investigating Or Analysing Biological Materials (AREA)
Abstract
The invention relates to medicine, in particular to a method for determining the cobalt binding ability of the ischemic modified albumin and can be used for the diagnosis of pathological processes caused by tissue and organ hypoxia, the study of their pathogenesis and the determination of treatment methods.The method, according to the invention, includes the treatment of biological material with 0.50...0.75 mM solution of cobalt chloride, mixing for 5 min at room temperature, addition of incubation medium, containing, mM: dithiothreitol 10.0...20.0, HEPES buffer solution pH 7.4 10.0...25.0, sodium chloride 140.0...150.0 and heptanol 0.3...1.0, stirring for 2 min, measurement of optical density of the obtained complex at a wavelength of 485 nm and determination of cobalt binding ability of the ischemic modified albumin.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
MDA20090116A MD4054C1 (en) | 2009-11-06 | 2009-11-06 | Method for determining the cobalt binding ability of the ischemic modified albumin |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
MDA20090116A MD4054C1 (en) | 2009-11-06 | 2009-11-06 | Method for determining the cobalt binding ability of the ischemic modified albumin |
Publications (2)
Publication Number | Publication Date |
---|---|
MD4054B1 MD4054B1 (en) | 2010-07-30 |
MD4054C1 true MD4054C1 (en) | 2011-02-28 |
Family
ID=43569012
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
MDA20090116A MD4054C1 (en) | 2009-11-06 | 2009-11-06 | Method for determining the cobalt binding ability of the ischemic modified albumin |
Country Status (1)
Country | Link |
---|---|
MD (1) | MD4054C1 (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102507916A (en) * | 2011-11-07 | 2012-06-20 | 宁波美康生物科技股份有限公司 | Ischemia modified albumin liquid stabilization kit |
Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0413678A2 (en) * | 1989-08-17 | 1991-02-20 | Manfred Dr. Kessler | Method for the detection and determination of human serum albumin in biological fluids using fluorogenic cyanine dyes |
SU1677632A1 (en) * | 1989-09-10 | 1991-09-15 | Азербайджанский Государственный Медицинский Институт Им.Н.Нариманова | Method for examination of cardiovascular system |
SU1720004A1 (en) * | 1990-01-18 | 1992-03-15 | Научно-Исследовательский Институт Физико-Химической Медицины Мз Рсфср | Method for determining albumin-globulin blood coefficient |
RU2102756C1 (en) * | 1995-05-22 | 1998-01-20 | Сибирский медицинский университет | Method of prognosis of ischemic heart disease course |
RU2182709C2 (en) * | 2000-04-28 | 2002-05-20 | Уральский НИИ охраны материнства и младенчества МЗ РФ | Method or preclinical diagnosis of hepatodepressive syndrome in gestation |
MD3394G2 (en) * | 2007-01-09 | 2008-04-30 | Диомид ГЕРМАН | Method of spinal marrow infarction diagnosis |
-
2009
- 2009-11-06 MD MDA20090116A patent/MD4054C1/en not_active IP Right Cessation
Patent Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0413678A2 (en) * | 1989-08-17 | 1991-02-20 | Manfred Dr. Kessler | Method for the detection and determination of human serum albumin in biological fluids using fluorogenic cyanine dyes |
SU1677632A1 (en) * | 1989-09-10 | 1991-09-15 | Азербайджанский Государственный Медицинский Институт Им.Н.Нариманова | Method for examination of cardiovascular system |
SU1720004A1 (en) * | 1990-01-18 | 1992-03-15 | Научно-Исследовательский Институт Физико-Химической Медицины Мз Рсфср | Method for determining albumin-globulin blood coefficient |
RU2102756C1 (en) * | 1995-05-22 | 1998-01-20 | Сибирский медицинский университет | Method of prognosis of ischemic heart disease course |
RU2182709C2 (en) * | 2000-04-28 | 2002-05-20 | Уральский НИИ охраны материнства и младенчества МЗ РФ | Method or preclinical diagnosis of hepatodepressive syndrome in gestation |
MD3394G2 (en) * | 2007-01-09 | 2008-04-30 | Диомид ГЕРМАН | Method of spinal marrow infarction diagnosis |
Non-Patent Citations (9)
Title |
---|
Bhagavan N.V., Lai E.M., Rios P.A., et. al. Evaluation of Human Serum Albumin Cobalt Binding Assay for the Assesment of Myocardial Ischemia and * |
Bhagavan N.V., Lai E.M., Rios P.A., et. al. Evaluation of Human Serum Albumin Cobalt Binding Assay for the Assesment of Myocardial Ischemia and Myocardial Infarction. Clin. Chemistry, 2003, 49:4, p. 581-585 * |
Christenson R.H., Hong Duh Sh., Sanhal W.R., et. al. Caracteristics of an Albumin Cobalt Binding Test for Assesment of Acute Coronary Syndrome * |
Christenson R.H., Hong Duh Sh., Sanhal W.R., et. al. Caracteristics of an Albumin Cobalt Binding Test for Assesment of Acute Coronary Syndrome Patients: A Multicenter Study. Clin. Chemistry, 2001, 47:3, p. 464-470 * |
Myocardial Infarction. Clin. Chemistry, 2003, 49:4, p. 581-585. * |
Patients: A Multicenter Study. Clin. Chemistry, 2001, 47:3, p. 464-470. * |
Литус (Верхоланцева)Е.А. Влияние химической модификации, вызываемой действием окислителей, на Со - связывающую активность * |
Литус Е.А., Гаврилова О.Д.. Со-связующая способность нативного и обработанного гипохлорит-анионом сывороточного альбумина. Вестник * |
сывороточного альбумина. www.volgmen.ru/news/2007.06.07.a.php. * |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102507916A (en) * | 2011-11-07 | 2012-06-20 | 宁波美康生物科技股份有限公司 | Ischemia modified albumin liquid stabilization kit |
CN102507916B (en) * | 2011-11-07 | 2013-11-06 | 宁波美康生物科技股份有限公司 | Ischemia modified albumin liquid stabilization kit |
Also Published As
Publication number | Publication date |
---|---|
MD4054B1 (en) | 2010-07-30 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
FG4A | Patent for invention issued | ||
KA4A | Patent for invention lapsed due to non-payment of fees (with right of restoration) |