MD4054B1 -   Method for determining the capacity of altered albumin ischemic binding of cobalt - Google Patents

  Method for determining the capacity of altered albumin ischemic binding of cobalt

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Publication number
MD4054B1
MD4054B1 MDA20090116A MD20090116A MD4054B1 MD 4054 B1 MD4054 B1 MD 4054B1 MD A20090116 A MDA20090116 A MD A20090116A MD 20090116 A MD20090116 A MD 20090116A MD 4054 B1 MD4054 B1 MD 4054B1
Authority
MD
Moldova
Prior art keywords
ischemic
determining
cobalt
binding
albumin
Prior art date
Application number
MDA20090116A
Other languages
Romanian (ro)
Other versions
MD4054C1 (en
Inventor
Valentin Gudumac
Olga Tagadiuc
Original Assignee
Universitatea De Stat De Medicina Si Farmacie "Nicolae Testemitanu" Din Republica Moldova
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Universitatea De Stat De Medicina Si Farmacie "Nicolae Testemitanu" Din Republica Moldova filed Critical Universitatea De Stat De Medicina Si Farmacie "Nicolae Testemitanu" Din Republica Moldova
Priority to MDA20090116A priority Critical patent/MD4054C1/en
Publication of MD4054B1 publication Critical patent/MD4054B1/en
Publication of MD4054C1 publication Critical patent/MD4054C1/en

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Abstract

Inventia se refera la medicina, in particular lao metoda de determinare a capacitati albuminei ischemic modificate de legare a cobaltului si poate fi folosita pentru diagnosticarea proceselor patologice cauzate de hipoxia tesuturilor si organelor, cercetarea patogenezei acestora si stabilirea metodelor de tratament. Metoda, conform inventiei, include prelucrarea materialului biologic cu solutie de 0,50...0,75 mM de clorura de cobalt, agitarea timp de 5 min la temperatura camerei, adaugarea unui mediu de incubare, ce contine, mM: ditiotreitol 10,0...20,0, solutie tampon HEPES pH 7,4 10,0...25,0, clorura de sodiu 140,0...150,0 si heptanol 0,3...1,0, agitarea timp de 2 min, masurarea densitatii optice a complexului obtinut la o lungime de unda de 485 nm si determinarea capacitatii albuminei ischemic modificate de legare a cobaltului.The invention relates to medicine, in particular to the method of determining the ischemic albumin capacity modified by cobalt binding and can be used to diagnose pathological processes caused by hypoxia of tissues and organs, to investigate their pathogenesis and to establish treatment methods. The method according to the invention includes the processing of biological material with 0.50 to 0.75 mM solution of cobalt chloride, stirring for 5 min at room temperature, adding an incubation medium containing, mM: dithiothreitol 10, 0 ... 20.0, HEPES buffer solution pH 7.4 10.0 ... 25.0, sodium chloride 140.0 ... 150.0 and heptanol 0.3 ... 1.0, stirring for 2 min, measuring the optical density of the complex obtained at a wavelength of 485 nm and determining the ability of altered ischemic albumin for cobalt binding.

MDA20090116A 2009-11-06 2009-11-06 Method for determining the cobalt binding ability of the ischemic modified albumin MD4054C1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
MDA20090116A MD4054C1 (en) 2009-11-06 2009-11-06 Method for determining the cobalt binding ability of the ischemic modified albumin

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
MDA20090116A MD4054C1 (en) 2009-11-06 2009-11-06 Method for determining the cobalt binding ability of the ischemic modified albumin

Publications (2)

Publication Number Publication Date
MD4054B1 true MD4054B1 (en) 2010-07-30
MD4054C1 MD4054C1 (en) 2011-02-28

Family

ID=43569012

Family Applications (1)

Application Number Title Priority Date Filing Date
MDA20090116A MD4054C1 (en) 2009-11-06 2009-11-06 Method for determining the cobalt binding ability of the ischemic modified albumin

Country Status (1)

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MD (1) MD4054C1 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102507916B (en) * 2011-11-07 2013-11-06 宁波美康生物科技股份有限公司 Ischemia modified albumin liquid stabilization kit

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATA194989A (en) * 1989-08-17 1990-04-15 Koller Ernst METHOD FOR DETECTING AND DETERMINING HUMAN SERUM ALBUMINE IN BIOLOGICAL LIQUIDS WITH THE AID OF FLUOROGENIC CYANINE DYES
SU1677632A1 (en) * 1989-09-10 1991-09-15 Азербайджанский Государственный Медицинский Институт Им.Н.Нариманова Method for examination of cardiovascular system
SU1720004A1 (en) * 1990-01-18 1992-03-15 Научно-Исследовательский Институт Физико-Химической Медицины Мз Рсфср Method for determining albumin-globulin blood coefficient
RU2102756C1 (en) * 1995-05-22 1998-01-20 Сибирский медицинский университет Method of prognosis of ischemic heart disease course
RU2182709C2 (en) * 2000-04-28 2002-05-20 Уральский НИИ охраны материнства и младенчества МЗ РФ Method or preclinical diagnosis of hepatodepressive syndrome in gestation
MD3394G2 (en) * 2007-01-09 2008-04-30 Диомид ГЕРМАН Method of spinal marrow infarction diagnosis

Also Published As

Publication number Publication date
MD4054C1 (en) 2011-02-28

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FG4A Patent for invention issued
KA4A Patent for invention lapsed due to non-payment of fees (with right of restoration)