MD3781F1 - Process for obtaining an antioxidant thermostable preparation from Spirulina platensis cyanobacterium biomass - Google Patents
Process for obtaining an antioxidant thermostable preparation from Spirulina platensis cyanobacterium biomassInfo
- Publication number
- MD3781F1 MD3781F1 MDA20080079A MD20080079A MD3781F1 MD 3781 F1 MD3781 F1 MD 3781F1 MD A20080079 A MDA20080079 A MD A20080079A MD 20080079 A MD20080079 A MD 20080079A MD 3781 F1 MD3781 F1 MD 3781F1
- Authority
- MD
- Moldova
- Prior art keywords
- obtaining
- biomass
- spirulina platensis
- preparation
- antioxidant
- Prior art date
Links
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention refers to biotechnology, particularly to a process for obtaining an antioxidant preparation from Spirulina platensis cyanobacterium biomass, used in the pharmaceutical industry and cosmetology. The process, according to the invention, includes cultivation of Spirulina platensis cyanobacterium biomass in open-type reactors on a Gromov culture medium with addition of the coordinative compound with the tartaric acid and imidazole [Mn(COO(CHOH)2COO(C3N2H4)2(H2O)2]ÀnH2O in the concentration of 20 mg/L at a temperature of 30...35?C, separation of biomass from the culture medium, destruction of cells by repeated congelation-decongelation, extraction of proteic fraction with 0,1 M Na-phosphatic buffer at a pH of 7,8...8,0 with addition of 10 mM EDTA, fractionation of proteic fracture by isoelectric precipitation at a pH of 3,8...4,0, centrifugation with subsequent separation of the precipitate, concentration of the supernatant liquid containing superoxidedismutase and fricocyanine using the Mr 40000 Da polyethylene glycol reagent and congelation. The result consists in obtaining a thermostable preparation with high antioxidant activity.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
MDA20080079A MD3781G2 (en) | 2008-03-18 | 2008-03-18 | Process for obtaining an antioxidant thermostable preparation from Spirulina platensis cyanobacterium biomass |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
MDA20080079A MD3781G2 (en) | 2008-03-18 | 2008-03-18 | Process for obtaining an antioxidant thermostable preparation from Spirulina platensis cyanobacterium biomass |
Publications (2)
Publication Number | Publication Date |
---|---|
MD3781F1 true MD3781F1 (en) | 2008-12-31 |
MD3781G2 MD3781G2 (en) | 2009-07-31 |
Family
ID=40255083
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
MDA20080079A MD3781G2 (en) | 2008-03-18 | 2008-03-18 | Process for obtaining an antioxidant thermostable preparation from Spirulina platensis cyanobacterium biomass |
Country Status (1)
Country | Link |
---|---|
MD (1) | MD3781G2 (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
MD4319C1 (en) * | 2013-10-17 | 2015-08-31 | Государственный Университет Молд0 | Bis[(iminodiacetate)oxothiomolybdate(V)]tetraphenylphosphonium hydrate and process for cultivation of Spirulina platensis cyanobacterium with the use thereof |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
MD4191C1 (en) * | 2011-12-14 | 2013-07-31 | Государственный Университет Молд0 | Process for extraction of phycocyanin from Spirulina platensis alga biomass |
MD4320C1 (en) * | 2013-10-17 | 2015-08-31 | Государственный Университет Молд0 | Process for determining the amount of Nostoc linckia cyanobacterium biomass |
Family Cites Families (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
FR2656874B1 (en) * | 1990-01-11 | 1992-04-03 | Commissariat Energie Atomique | PROCESS FOR THE PRODUCTION AND EXTRACTION OF ANTI-OXIDANTS FROM A CULTURE OF MICROORGANISMS AND PHOTOBIOREACTOR FOR THE IMPLEMENTATION OF THIS PROCESS. |
FR2706465B1 (en) * | 1993-06-11 | 1995-08-25 | Heliosynthese Sa | Process for the production and extraction of thermostable superoxide dismutases from a culture of photosynthetic microorganisms. |
MD1008G2 (en) * | 1997-04-30 | 1999-06-30 | Государственный Университет Молд0 | Method for obtaining the phyocyanine |
-
2008
- 2008-03-18 MD MDA20080079A patent/MD3781G2/en not_active IP Right Cessation
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
MD4319C1 (en) * | 2013-10-17 | 2015-08-31 | Государственный Университет Молд0 | Bis[(iminodiacetate)oxothiomolybdate(V)]tetraphenylphosphonium hydrate and process for cultivation of Spirulina platensis cyanobacterium with the use thereof |
Also Published As
Publication number | Publication date |
---|---|
MD3781G2 (en) | 2009-07-31 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
WO2007119213A3 (en) | A culture medium and pharmaceutical composition for regenerating cartilage tissue, a method, uses and products related thereto | |
AU2012274478A8 (en) | Method for amplifying NK cells | |
MX2018006063A (en) | Media and fermentation methods for producing polysaccharides in bacterial cell culture. | |
WO2011116922A9 (en) | Thermoreactive substrate with microgels, method for its preparation, and culture method for biological cells | |
WO2008136398A1 (en) | Cell culture method using amino acid-enriched medium | |
WO2014093505A3 (en) | Acp-mediated production of fatty acid derivatives | |
WO2009147200A3 (en) | Method for the supply of growth components to cell cultures | |
CN109105362B (en) | In-vitro deciduous tooth preserving fluid and preparation method thereof | |
MD3781F1 (en) | Process for obtaining an antioxidant thermostable preparation from Spirulina platensis cyanobacterium biomass | |
Chouaib et al. | Towards the standardization of mesenchymal stem cell secretome-derived product manufacturing for tissue regeneration | |
Chiong et al. | Microalgal-based protein by-products: Extraction, purification, and applications | |
RU2012107674A (en) | BIOTRANSPLANT FOR RESTORING BONE TISSUE VOLUME AT DEGENERATIVE DISEASES AND TRAUMATIC BONE DAMAGES AND METHOD FOR ITS OBTAINING | |
CN110669724A (en) | Saccharum embryo culture solution and preparation method thereof | |
MD3129G2 (en) | Process for Spirulina platensis cyanobacterium biomass obtaining | |
CN111560451B (en) | Economical and efficient method for batch determination of intestinal tract thalli external growth and interaction rule | |
CN105331656B (en) | The preparation method of the hidden dinoflagellate exocellular polysaccharide of Kou Shi and the application of the exocellular polysaccharide | |
CN104910254B (en) | Applications of the dipeptides Pro-Asp in terms of animal liver cell secretion IGF-1 is promoted | |
MD3128G2 (en) | Process for Spirulina platensis biomass obtaining | |
MD3171F1 (en) | Process for Spirulina platensis cyanobacterium biomass obtaining | |
ATE466023T1 (en) | MANNOSE-SPECIFIC ADHESINS AND THEIR USE | |
TR200602727A2 (en) | A method and a tissue substance for the treatment of skin defects and aging | |
NZ596746A (en) | Blood-derived supplement with platelets activator for cell cultivation media | |
Quintero Sierra et al. | In Vitro Study of a Novel Vibrio alginolyticus-Based Collagenase for Future Medical Application | |
MD191Z5 (en) | Nutrient medium for the cultivation of cells of animal origin | |
CN102675436A (en) | Extraction method for chlorella vulgaris metallothioneins with whitening functions |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
FG4A | Patent for invention issued | ||
KA4A | Patent for invention lapsed due to non-payment of fees (with right of restoration) | ||
MM4A | Patent for invention definitely lapsed due to non-payment of fees |