MD3570F1 - Procedeu de obtinere a ergosterolului din drojdii Saccharomyces - Google Patents
Procedeu de obtinere a ergosterolului din drojdii Saccharomyces Download PDFInfo
- Publication number
- MD3570F1 MD3570F1 MDA20070243A MD20070243A MD3570F1 MD 3570 F1 MD3570 F1 MD 3570F1 MD A20070243 A MDA20070243 A MD A20070243A MD 20070243 A MD20070243 A MD 20070243A MD 3570 F1 MD3570 F1 MD 3570F1
- Authority
- MD
- Moldova
- Prior art keywords
- ergosterol
- obtaining
- biomass
- alcohol
- minutes
- Prior art date
Links
- OILXMJHPFNGGTO-UHFFFAOYSA-N (22E)-(24xi)-24-methylcholesta-5,22-dien-3beta-ol Natural products C1C=C2CC(O)CCC2(C)C2C1C1CCC(C(C)C=CC(C)C(C)C)C1(C)CC2 OILXMJHPFNGGTO-UHFFFAOYSA-N 0.000 title abstract 4
- RQOCXCFLRBRBCS-UHFFFAOYSA-N (22E)-cholesta-5,7,22-trien-3beta-ol Natural products C1C(O)CCC2(C)C(CCC3(C(C(C)C=CCC(C)C)CCC33)C)C3=CC=C21 RQOCXCFLRBRBCS-UHFFFAOYSA-N 0.000 title abstract 4
- OQMZNAMGEHIHNN-UHFFFAOYSA-N 7-Dehydrostigmasterol Natural products C1C(O)CCC2(C)C(CCC3(C(C(C)C=CC(CC)C(C)C)CCC33)C)C3=CC=C21 OQMZNAMGEHIHNN-UHFFFAOYSA-N 0.000 title abstract 4
- DNVPQKQSNYMLRS-NXVQYWJNSA-N Ergosterol Natural products CC(C)[C@@H](C)C=C[C@H](C)[C@H]1CC[C@H]2C3=CC=C4C[C@@H](O)CC[C@]4(C)[C@@H]3CC[C@]12C DNVPQKQSNYMLRS-NXVQYWJNSA-N 0.000 title abstract 4
- DNVPQKQSNYMLRS-SOWFXMKYSA-N ergosterol Chemical compound C1[C@@H](O)CC[C@]2(C)[C@H](CC[C@]3([C@H]([C@H](C)/C=C/[C@@H](C)C(C)C)CC[C@H]33)C)C3=CC=C21 DNVPQKQSNYMLRS-SOWFXMKYSA-N 0.000 title abstract 4
- 238000000034 method Methods 0.000 title abstract 4
- 240000004808 Saccharomyces cerevisiae Species 0.000 title abstract 3
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 abstract 6
- 239000002028 Biomass Substances 0.000 abstract 3
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 abstract 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 abstract 2
- MECHNRXZTMCUDQ-UHFFFAOYSA-N Vitamin D2 Natural products C1CCC2(C)C(C(C)C=CC(C)C(C)C)CCC2C1=CC=C1CC(O)CCC1=C MECHNRXZTMCUDQ-UHFFFAOYSA-N 0.000 abstract 1
- 238000001816 cooling Methods 0.000 abstract 1
- 229960002061 ergocalciferol Drugs 0.000 abstract 1
- 238000007710 freezing Methods 0.000 abstract 1
- 230000008014 freezing Effects 0.000 abstract 1
- 239000000203 mixture Substances 0.000 abstract 1
- 230000000737 periodic effect Effects 0.000 abstract 1
- 239000002243 precursor Substances 0.000 abstract 1
- 238000000926 separation method Methods 0.000 abstract 1
- 238000003756 stirring Methods 0.000 abstract 1
- 239000006200 vaporizer Substances 0.000 abstract 1
- MECHNRXZTMCUDQ-RKHKHRCZSA-N vitamin D2 Chemical compound C1(/[C@@H]2CC[C@@H]([C@]2(CCC1)C)[C@H](C)/C=C/[C@H](C)C(C)C)=C\C=C1\C[C@@H](O)CCC1=C MECHNRXZTMCUDQ-RKHKHRCZSA-N 0.000 abstract 1
- 235000001892 vitamin D2 Nutrition 0.000 abstract 1
- 239000011653 vitamin D2 Substances 0.000 abstract 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract 1
Landscapes
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Steroid Compounds (AREA)
Abstract
Inventia se refera la biotehnologie, si anume la procedeele de obtinere a ergosterolului, precursoral vitaminei D2, din drojdii Saccharomyces. Procedeul, conform inventiei, include congelarea biomasei umede de drojdii timp de 30…60 min, la temperatura de -18°C, saponificarea biomasei cu amestec de hidroxid de sodiu, apa si alcool izopropilic luate in raport respectiv de 25:50:25 intr-o baie deaburi cu temperatura de 75°C timp de 30…60 min, racirea biomasei si adaugarea alcoolului izopropilic, agitarea periodica timp de 30 min, separareaextractului nesaponificat ce contine ergosterol si alcool, si distilarea alcoolului intr-un vaporizator cu vid. Rezultatul inventiei consta in sporirea cu 18,3...31,9% a continutului de ergosterol extras si micsorarea duratei procesului de obtinere a lui.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| MDA20070243A MD3570G2 (ro) | 2007-09-07 | 2007-09-07 | Procedeu de obţinere a ergosterolului din drojdii Saccharomyces |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| MDA20070243A MD3570G2 (ro) | 2007-09-07 | 2007-09-07 | Procedeu de obţinere a ergosterolului din drojdii Saccharomyces |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| MD3570F1 true MD3570F1 (ro) | 2008-04-30 |
| MD3570G2 MD3570G2 (ro) | 2008-11-30 |
Family
ID=39365465
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MDA20070243A MD3570G2 (ro) | 2007-09-07 | 2007-09-07 | Procedeu de obţinere a ergosterolului din drojdii Saccharomyces |
Country Status (1)
| Country | Link |
|---|---|
| MD (1) | MD3570G2 (ro) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| MD299P2 (ro) * | 1995-01-30 | 1996-01-31 | Institutul De Cercetari Stiintifice, Selectie Si Tehnologii Pentru Pomicultura | Soi de piersic Moldavskii jioltâi |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2080389C1 (ru) * | 1993-02-10 | 1997-05-27 | Акционерное общество открытого типа "Фармакон" | Способ получения эргостерина |
-
2007
- 2007-09-07 MD MDA20070243A patent/MD3570G2/ro not_active IP Right Cessation
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| MD299P2 (ro) * | 1995-01-30 | 1996-01-31 | Institutul De Cercetari Stiintifice, Selectie Si Tehnologii Pentru Pomicultura | Soi de piersic Moldavskii jioltâi |
Also Published As
| Publication number | Publication date |
|---|---|
| MD3570G2 (ro) | 2008-11-30 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FG4A | Patent for invention issued | ||
| KA4A | Patent for invention lapsed due to non-payment of fees (with right of restoration) | ||
| MM4A | Patent for invention definitely lapsed due to non-payment of fees |