MD1799G2 - Метод оценки устойчивости генотипов тритикале к фузариозу - Google Patents
Метод оценки устойчивости генотипов тритикале к фузариозу Download PDFInfo
- Publication number
- MD1799G2 MD1799G2 MDA20000061A MD20000061A MD1799G2 MD 1799 G2 MD1799 G2 MD 1799G2 MD A20000061 A MDA20000061 A MD A20000061A MD 20000061 A MD20000061 A MD 20000061A MD 1799 G2 MD1799 G2 MD 1799G2
- Authority
- MD
- Moldova
- Prior art keywords
- triticale
- fusariose
- resistance
- genotypes
- estimating
- Prior art date
Links
- 241000228158 x Triticosecale Species 0.000 title abstract 5
- 208000004770 Fusariosis Diseases 0.000 title abstract 4
- 235000019714 Triticale Nutrition 0.000 title abstract 4
- DGMPVYSXXIOGJY-UHFFFAOYSA-N Fusaric acid Chemical compound CCCCC1=CC=C(C(O)=O)N=C1 DGMPVYSXXIOGJY-UHFFFAOYSA-N 0.000 abstract 2
- 230000003321 amplification Effects 0.000 abstract 2
- 239000012634 fragment Substances 0.000 abstract 2
- 238000003199 nucleic acid amplification method Methods 0.000 abstract 2
- 238000001228 spectrum Methods 0.000 abstract 2
- 239000003155 DNA primer Substances 0.000 abstract 1
- 241000196324 Embryophyta Species 0.000 abstract 1
- 229920001222 biopolymer Polymers 0.000 abstract 1
- 235000013339 cereals Nutrition 0.000 abstract 1
- 238000001962 electrophoresis Methods 0.000 abstract 1
- 239000002773 nucleotide Substances 0.000 abstract 1
- 125000003729 nucleotide group Chemical group 0.000 abstract 1
- 238000003752 polymerase chain reaction Methods 0.000 abstract 1
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
Изобретение относится к сельскому хозяйству, в частности к генетике и селекции растений и может быть использовано для оценки устойчивости генотипов тритикале к фузариозу.Сущность изобретения состоит в оценке устойчивости генотипов тритикале к фузариозу путем электрофоретического анализа биополимеров в корешках тритикале после обработки зерен 0,01%-ной фузариевой кислотой в течение 18 часов. Aнализируют электрофоретический спектр продуктов амплификации фрагментов ДНК в результате цепной реакции полимеразы при использовании олигонуклеотидных праймеров OPA.2: 5′TGCCGAGCTG-3′ и OPA.3: 5′AGTCAGCCAC-3′. Растения тритикале, у которых электрофоретический спектр продуктов амплификации фрагментов ДНК с весом 100…400 пар нуклеотодов не изменяется, оцениваются как устойчивые к фузариозу.Ï. ôîðìóëû: 1Ôèã.: 2
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| MDA20000061A MD1799G2 (ru) | 2000-04-07 | 2000-04-07 | Метод оценки устойчивости генотипов тритикале к фузариозу |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| MDA20000061A MD1799G2 (ru) | 2000-04-07 | 2000-04-07 | Метод оценки устойчивости генотипов тритикале к фузариозу |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| MD1799F1 MD1799F1 (en) | 2001-12-31 |
| MD1799G2 true MD1799G2 (ru) | 2002-06-30 |
Family
ID=19739573
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MDA20000061A MD1799G2 (ru) | 2000-04-07 | 2000-04-07 | Метод оценки устойчивости генотипов тритикале к фузариозу |
Country Status (1)
| Country | Link |
|---|---|
| MD (1) | MD1799G2 (ru) |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| MD1261G2 (ru) * | 1997-11-14 | 2000-10-31 | Институт Генетики И Физиологии Растений Академии Наук Молдовы | Метод оценки устойчивости генотипов тритикале к фузариозу |
-
2000
- 2000-04-07 MD MDA20000061A patent/MD1799G2/ru not_active IP Right Cessation
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| MD1261G2 (ru) * | 1997-11-14 | 2000-10-31 | Институт Генетики И Физиологии Растений Академии Наук Молдовы | Метод оценки устойчивости генотипов тритикале к фузариозу |
Non-Patent Citations (1)
| Title |
|---|
| Попов Ю.В. Шкала учета корневых гнилей. В: Зерновое хозяйство. 1985, № 9, с. 21 * |
Also Published As
| Publication number | Publication date |
|---|---|
| MD1799F1 (en) | 2001-12-31 |
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| Date | Code | Title | Description |
|---|---|---|---|
| MM4A | Patent for invention definitely lapsed due to non-payment of fees |