MD1799F1 - Method of estimating the triticale genotypes resistance to fusariose - Google Patents
Method of estimating the triticale genotypes resistance to fusariose Download PDFInfo
- Publication number
- MD1799F1 MD1799F1 MD20000061A MD20000061A MD1799F1 MD 1799 F1 MD1799 F1 MD 1799F1 MD 20000061 A MD20000061 A MD 20000061A MD 20000061 A MD20000061 A MD 20000061A MD 1799 F1 MD1799 F1 MD 1799F1
- Authority
- MD
- Moldova
- Prior art keywords
- triticale
- fusariose
- resistance
- genotypes
- estimating
- Prior art date
Links
- 241000228158 x Triticosecale Species 0.000 title abstract 5
- 208000004770 Fusariosis Diseases 0.000 title abstract 4
- 235000019714 Triticale Nutrition 0.000 title abstract 4
- DGMPVYSXXIOGJY-UHFFFAOYSA-N Fusaric acid Chemical compound CCCCC1=CC=C(C(O)=O)N=C1 DGMPVYSXXIOGJY-UHFFFAOYSA-N 0.000 abstract 2
- 230000003321 amplification Effects 0.000 abstract 2
- 239000012634 fragment Substances 0.000 abstract 2
- 238000003199 nucleic acid amplification method Methods 0.000 abstract 2
- 238000001228 spectrum Methods 0.000 abstract 2
- 239000003155 DNA primer Substances 0.000 abstract 1
- 241000196324 Embryophyta Species 0.000 abstract 1
- 229920001222 biopolymer Polymers 0.000 abstract 1
- 235000013339 cereals Nutrition 0.000 abstract 1
- 238000001962 electrophoresis Methods 0.000 abstract 1
- 239000002773 nucleotide Substances 0.000 abstract 1
- 125000003729 nucleotide group Chemical group 0.000 abstract 1
- 238000003752 polymerase chain reaction Methods 0.000 abstract 1
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
he invention relates to agriculture, in particular to plant genetics and selection and may be used for the estimation of triticale genotype resistance to fusariose. Summary of the invention consists in estimating resistance of the triticale genotypes to fusariose by electrophoretic analysis of the biopolymers in the triticale radicles after treatment of grains with 0,01% fusaric acid during 18 hours. It is analysed the electrophoretic spectrum of the DNA fragments amplification products as a result of polymerase chain reaction when using oligonucleotide primers OPA.2: 5'TGCCGAGCTG-3' and OPA.3: 5'AGTCAGCCAC-3'. The triticale plants, to which the electrophoretic spectrum of the DNA fragments amplification products with a weight of 100...400 nucleotide pairs is not changed, are estimated as resistant to fusariose.Claims: 1Fig.: 2
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| MDA20000061A MD1799G2 (en) | 2000-04-07 | 2000-04-07 | Method of estimating the triticale genotypes resistance to fusariose |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| MDA20000061A MD1799G2 (en) | 2000-04-07 | 2000-04-07 | Method of estimating the triticale genotypes resistance to fusariose |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| MD1799F1 true MD1799F1 (en) | 2001-12-31 |
| MD1799G2 MD1799G2 (en) | 2002-06-30 |
Family
ID=19739573
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MDA20000061A MD1799G2 (en) | 2000-04-07 | 2000-04-07 | Method of estimating the triticale genotypes resistance to fusariose |
Country Status (1)
| Country | Link |
|---|---|
| MD (1) | MD1799G2 (en) |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| MD1261G2 (en) * | 1997-11-14 | 2000-10-31 | Институт Генетики И Физиологии Растений Академии Наук Молдовы | Method for evaluation of the triticale genotypes resistance to fusariose |
-
2000
- 2000-04-07 MD MDA20000061A patent/MD1799G2/en not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| MD1799G2 (en) | 2002-06-30 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| MM4A | Patent for invention definitely lapsed due to non-payment of fees |