MD1067Y - Method for simulation of pemphigus-type lesions - Google Patents
Method for simulation of pemphigus-type lesions Download PDFInfo
- Publication number
- MD1067Y MD1067Y MDS20160016A MDS20160016A MD1067Y MD 1067 Y MD1067 Y MD 1067Y MD S20160016 A MDS20160016 A MD S20160016A MD S20160016 A MDS20160016 A MD S20160016A MD 1067 Y MD1067 Y MD 1067Y
- Authority
- MD
- Moldova
- Prior art keywords
- pemphigus
- simulation
- alcohol
- lyophilized
- centrifuged
- Prior art date
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- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Abstract
The invention relates to the field of experimental medicine, in particular to a method for simulation of an autoimmune cutaneous disease, such as pemphigus, to study the disease pathogenesis and to develop new treatment methods.Summary of the invention consists in the separation of bovine esophageal mucosa, grinding and homogenization of mucosa three times in phosphate buffer with pH=7 in a ratio of 1:2, at 4°C, then the homogenate is centrifuged, the sediment is washed three times with phosphate buffer, lyophilized and subjected to extraction in 70% ethyl alcohol with addition of 5 ml of alcohol per gram of dry mass, stirred for 60 min and centrifuged, the alcohol extract is decanted and subjected to dialysis for 8 hours, then is lyophilized, afterwards is mixed in equal volumes of protein extract, sterile glycerol and oily vitamin solution, containing per 1 ml: A - 50000 IU, D3 - 25,000 IU, E - 20 mg, F - 5 mg, then the mixture is administered to the rats at 0.1 ml intradermally in different places, once every two weeks, the procedure is repeated 12 times.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| MDS20160016A MD1067Z (en) | 2016-02-11 | 2016-02-11 | Method for simulation of pemphigus-type lesions |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| MDS20160016A MD1067Z (en) | 2016-02-11 | 2016-02-11 | Method for simulation of pemphigus-type lesions |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| MD1067Y true MD1067Y (en) | 2016-08-31 |
| MD1067Z MD1067Z (en) | 2017-03-31 |
Family
ID=56855226
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MDS20160016A MD1067Z (en) | 2016-02-11 | 2016-02-11 | Method for simulation of pemphigus-type lesions |
Country Status (1)
| Country | Link |
|---|---|
| MD (1) | MD1067Z (en) |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2316033C1 (en) * | 2006-06-05 | 2008-01-27 | Владимир Васильевич Маркин | Method of manufacturing holographic screen for demonstrating three-dimensional color imagines |
| RU2316005C1 (en) * | 2006-09-07 | 2008-01-27 | Государственное учреждение Московский областной научно-исследовательский клинический институт им. М.Ф. Владимирского (МОНИКИ им. М.Ф. Владимирского) | Method for estimating pemphigus treatment effectiveness |
| RU2316006C1 (en) * | 2006-09-07 | 2008-01-27 | Государственное учреждение Московский областной научно-исследовательский клинический институт им. М.Ф. Владимирского (МОНИКИ им. М.Ф. Владимирского) | Method for estimating pemphigus clinical course severity degree |
| RU2429482C1 (en) * | 2010-06-03 | 2011-09-20 | Государственное учреждение Московский областной научно-исследовательский клинический институт им. М.Ф. Владимирского (ГУ МОНИКИ им. М.Ф. Владимирского) | Method for prediction of clinical course of autoimmune pemphigus |
| RU2473911C1 (en) * | 2011-05-16 | 2013-01-27 | Николай Львович Шимановский | Method for detection of steroid resistance in patients with true acantolytic pemphigus on basis of studying secondary messenger system |
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2016
- 2016-02-11 MD MDS20160016A patent/MD1067Z/en not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| MD1067Z (en) | 2017-03-31 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FG9Y | Short term patent issued | ||
| KA4Y | Short-term patent lapsed due to non-payment of fees (with right of restoration) |