MA35294B1 - Probes and primers for detecting the her2 gene in multiplexed format and its applications in the choice of her2 breast cancer treatment - Google Patents

Probes and primers for detecting the her2 gene in multiplexed format and its applications in the choice of her2 breast cancer treatment

Info

Publication number
MA35294B1
MA35294B1 MA35508A MA35508A MA35294B1 MA 35294 B1 MA35294 B1 MA 35294B1 MA 35508 A MA35508 A MA 35508A MA 35508 A MA35508 A MA 35508A MA 35294 B1 MA35294 B1 MA 35294B1
Authority
MA
Morocco
Prior art keywords
her2
breast cancer
primers
choice
applications
Prior art date
Application number
MA35508A
Other languages
French (fr)
Inventor
Hassane Sefrioui El
Kottwitz Denise
El Amrani Manale
Moumen Abdeladim
Bouchoutrouch Nadia
Original Assignee
Mascir Moroccan Foundation For Advanced Science Innovation & Res
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Mascir Moroccan Foundation For Advanced Science Innovation & Res filed Critical Mascir Moroccan Foundation For Advanced Science Innovation & Res
Priority to MA35508A priority Critical patent/MA35294B1/en
Priority to PCT/MA2013/000023 priority patent/WO2014104867A1/en
Publication of MA35294B1 publication Critical patent/MA35294B1/en
Priority to TNP2015000292A priority patent/TN2015000292A1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/158—Expression markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Analytical Chemistry (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Hospice & Palliative Care (AREA)
  • Biophysics (AREA)
  • Oncology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

L’invention concerne le domaine du diagnostique du cancer du sein her2. Plus précisément, l’invention divulgue une méthode utilisant de nouveaux sondes et amorces pour détecter le gène her2 en format multiplexe et ses applications dans le choix de traitement du cancer du sein. Le protocole de la méthode utilise la pcr quantitative en temps réel (qpcr) sous un format simplexe ou multiplexe. Les gènes contrôles sélectionnés dans la présente convention (rpl3o, rplp37, et mrpl19) appartiennent à la famille des gènes codants pour des protéines ribosomales et sont décrits ici pour la première fois dans la quantification de her2.The invention relates to the field of the diagnosis of her2 breast cancer. More specifically, the invention discloses a method using novel probes and primers for detecting the her2 gene in multiplexed format and its applications in the choice of breast cancer treatment. The protocol of the method uses the real-time quantitative pcr (qpcr) in a simplex or multiplexed format. The control genes selected in this convention (rpl3o, rplp37, and mrpl19) belong to the family of coding genes for ribosomal proteins and are described here for the first time in her2 quantification.

MA35508A 2012-12-27 2012-12-27 Probes and primers for detecting the her2 gene in multiplexed format and its applications in the choice of her2 breast cancer treatment MA35294B1 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
MA35508A MA35294B1 (en) 2012-12-27 2012-12-27 Probes and primers for detecting the her2 gene in multiplexed format and its applications in the choice of her2 breast cancer treatment
PCT/MA2013/000023 WO2014104867A1 (en) 2012-12-27 2013-08-08 Probes and primers for detection of the her2 gene and a regulator gene (ribosomal) in multiplex format: use in selecting treatment for her2 breast cancer
TNP2015000292A TN2015000292A1 (en) 2012-12-27 2015-06-25 PROBES AND PRIMERS FOR DETECTING THE HER2 GENE AND A MULTIPLEX FORMAT CONTROL (RIBOSOMAL) GENE: APPLICATIONS IN THE CHOICE OF TREATING HER2 BREAST CANCER

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
MA35508A MA35294B1 (en) 2012-12-27 2012-12-27 Probes and primers for detecting the her2 gene in multiplexed format and its applications in the choice of her2 breast cancer treatment

Publications (1)

Publication Number Publication Date
MA35294B1 true MA35294B1 (en) 2014-08-01

Family

ID=49724642

Family Applications (1)

Application Number Title Priority Date Filing Date
MA35508A MA35294B1 (en) 2012-12-27 2012-12-27 Probes and primers for detecting the her2 gene in multiplexed format and its applications in the choice of her2 breast cancer treatment

Country Status (3)

Country Link
MA (1) MA35294B1 (en)
TN (1) TN2015000292A1 (en)
WO (1) WO2014104867A1 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
MA37437B1 (en) * 2014-10-17 2016-12-30 Mascir (Morrocan Found For Advanced Science Innovation & Research) Breast cancer diagnostic kit in qpcr multiplex reaction and its application in the evaluation of the status of the her2 protein and the choice of treatment
MA41742B1 (en) * 2017-12-29 2020-02-28 Moroccan Foundation For Advanced Science Innovation And Res Mascir Method for the determination of the positivity threshold of the overexpression of the her2 protein in breast cancer and other cancers.

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2574447A1 (en) * 2004-07-15 2006-01-26 University Of Utah Research Foundation Housekeeping genes and methods for identifying the same

Also Published As

Publication number Publication date
TN2015000292A1 (en) 2016-10-03
WO2014104867A1 (en) 2014-07-03

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