LV12487B - Method of diagnosis of missed abortion - Google Patents

Abstract

Invention relates to the field of medicine and particularly obstetrics. It concerns methods for diagnosing missed abortion. A method provides testing blood samples of pregnant woman. Two test-tubes are filled with leukocyte suspension in concentration 1 million cells per 1 ml and native serum of the woman in test. A combined preparation (rimolan) containing chorionic gonadotropin, progesterone, prolactin, estradiol and luteinizing hormone is added to the first test-tube for determination of a level of sensibilization of leukocytes. A presence of anti-sensibilizing antibodies against rimolan is determined in the second test-tube. A difference P between sensibilization index S and antibodies index Ba is calculated. Where S is equal or less than 30 and P is -10 or lesser ( -15, -20 etc.) the missed abortion is diagnosed. The method provides counting non-adhering cells in both test-tubes in presence of antigen - rimolan. This method allows to diagnose missed abortion in early stage of pregnancy - between the third and the sixth week.

Description

1 LV 12487

Csnoco® FluoroCTMKiH NFLMiMiMiNe (Misssd abortcoin) ye © M © EiiH © m H3o6peTeHHe othochtcji κ o6jiacTH MezmmiHM;

Ilpo6jieMa AHarHOCTHKH rH6eRiJio ^ a JUMeTca floCTaTOHHO cjiohchoh, ocoEeHHO Ha paHHHx cpOKax SepeMeHHOCTH (3-6 Hezzejib). CBeepeMeHHo He ztHarHocTHpoBaHHaa 3aMepma 6epeMeHHOCTb (niOejib njiozza) conpoBoamaeTca pa3BHTHeM ocroacHeHHH y 6epeMeHHofi: 3? H3BecTeH cnoco6 ληβγηοοτηκη rnSejiH njio / za c noMOim > io οοηο ^ Φηη: There is a difference between cepzuīe SHeHHe. O / iHaKO cep / me6neHHe y njiozza onpeaejiaeTca He paHHee 6 Hezzejib 6epeMeHHOCTH. flaHHbif Μετοζζ zmarHocTHKH aBJiaeTca aHanoroM Η3θ6ρβτβΗΐΜ. H3BecreH cnoco6 αηηγηοοτηκη πιδβΠΗ njiozza nyxeM HccjieaoBaHHH ypoBHH xopHOHHHecKoro roHaziOTponHHa (ΧΓ). Onpezzeje HHyHH rEtMOHa JieacHT b ocHOBe Bcex τεοτοΒ zum paHHeft zmarHOCTHKH 6epeMeHHOCTH (6HOJionHHHKHx, HMMYHOXHMHHHCKHX, paflHOHMMHHOOHHHeCKHX). HaH6onbIIiyiO flHaTHOCTHHeCKyiO ueHHOCTb 3TOT τβοτ ΗΜββτb paHHHe cpoKH 6epeMeHHocTH 6-10 Hezzejm (1,2,3). H3BecTHO, hto y 20% aceHMHHc peryjMpHMM MeHCipyajibHbiM hhkjiom ΧΓ onpezzejiaeTca b KOHneHTpanHH 1250-2500 ME / ml cnycT? 6-7 Goodbye 6epeMeHHOCTH), y 35% - c 51 zma (6cuiee 7 Hezzejib 6epeMeHH0CTH). KjiHHHHecKaa ηηΦορμητηβηοοή. onpezzeneHiM cozzepacaHH »xopHOHHHecxoro roHazioTponHHa npn cpoice 6epe cocTajiaeT 74%. (4.5). J κο30ηηι ιη Μβτοζι «BJMerca προτοτκποΜ H3o6pereHHa.

OzmaKo H3BecTHo, ητο πρκ ΓΗββΛΗ njiozza eine b τβπβΗΗΗ 2-x Hezzejit b CHopoTKe κροΒΗ SepeMeHHOH onpeztejiaeTca φΗ3ΗθποπΐΗβοκ3 »KOHiieHTpamM ziaHHoro ropMOHa, κοτορω οιπη6οηηο CBKweTejibCTByeT ο ηβπηηηη > khboh 6epeMeHHOCTH (1). 3t0 npHBOZOn K OHffl6KaM B ΖΖΗΒΓΗΟΟΤΗΚβ ΤβΗβΗΗΗ ββρβΜβΗΗΟΟΤΗ. ΒβρβΜβΗΗΜ aceHiHHHa χολητ c πογη6πιημ njio ^ oM β τβΗβΗΗΛ 15 / ΖΗβη η 6oaee. H3BecTeH nOpenThe HHa οποητβηηβιχ a6oproB, coaepMcaiiiHft COMIMKC SnojionHecKH aKTHBHBix ropMOHOBt xopHOHHHecKHH roHazKnpomiH, nporecTepoH, npojiaKTHH, 3CTpaaHOJi, yorHHH3Hypyrim RopMOH. TlaHHbiH npenapT Ha3biBaeTca PHMOJIAH HcnoJib3yeTca zum JieneHHH οποητβηη & χ a6opTOB (6). Ijibio H3o6peTeHHa aBjiaerca noBbiuieHH aoTOBepHoera ziHaraocTHKH πρκ ΓΗβεπη nzioia y 6epeMeHHofi aceHnmHbi npn cpOKax 6epeMeHHOcra 3-6 nejib. riocTaBJieHHaa nejib ziocTHraeTca 3a cneT τογο, hto γοτοβητ JieHKonHTapHyio B3Becb H3 κροΒΗ 6epeMeHHOŽ H3 patient 10 mjih β I mji h HaTHBHyio cHBopoTKy κροΒΗ 6epMeHHoii; 5epyT 2 προβΗρκκ b nepBoft προβκρκε onpeziejMiOT CTeneHb ceHCH6rniH3anHH jieāKoiiHTos SepeMeHHon κ npenapaTy PHMOJIAH, bo 2-h προβκρκε onpeziejiaiOT HairanHe 6jioKHpyiouiHx ceHcn6HJiH3auHio aHTHTen b ayTOCbiBopoTKe 6epeMeHHoft πρκ zio6aBJieHHH b peaKiļHK) technology '2 PHMOJIAH η onpeflejunoT pa3HHny noKa3arejien CTenenn ceHCH6njiH3anHH h KOJiHHecTsa 6jiOKHpyiomHx aHTHTeji of φopMyJIe: P = C-Ea, r e e C - noKa3aTeJe ceHCn6HJiH3anHH JieftKOHHTOB 6epeMeHHOH κ npenapaiy PHMOJIAH; Ea - noKa3aTejib HaJiHHna b CbmopoTKe OepeMeHHoft 6jOKHpyiomHX aHTHTe npn aoSaBJieHHH b headKiHiNHi nPaPaPa PHMOJIAH; P - pa3HHna noKajarejieii, h npn noKa3aTeJie C paBHOM 30 h MeHHee h noKa3areJie P paBHOM -10 h MeHee (-15; -20 η t.) To y 6epeMeHHoft aHarHOCTHpyerca rnSeJib njioa.

Cnoco6 ocHOBaH Ha npeKpaineHHH treasure3HH (npHJiHnamia) nadiiKonHTOB x CTeKJlHHHblM HJIH ia JiaCTHKO BbIM ΠΟΒβρΧΗΟΟΤΗΜ B npHCyTCTBHH ΟΠεηΗφΗΗεΟΚΟΓΟ

aHTHreHa. There is a need for the following: HHKy6aiiHH co ΟΠβΙίΗφΗΗεΟΚΗΜ ΒΗΤΗΓβΗΟΜ HJIH BHHBJieHH Κ H8My C8HCH6HJIH3aHHH JieŽKOIIHTOB naiuOHTI. B KanecTBe aHTHreHa Hcnonb30BaH npenapar PHMOJIAH ..

HeadManagement; LCp 199

2.0.85% XJ10pHCTHH aMMOHHH 3.3-5% pacTsop yicycycHOH khcjioth c MeTHJieHOBbiM chhhm min noacHeTa ηβίίκοηΗΤΟΒ 4-ΠηοΦκιιη3ηροββηημη npenapaT PHMOJIAH.

PeaKTHB PHMOJIAH cjieayeT pacTBOpHTb b 3 mji 199 cpeabi; B icanecTse aHarHOCTHKyMa pa3BeaeHHbiH PHMOJIAH pa3JiHTb from 1 mJ b 3ΠΗΗηορφΗ n xpaHHTb b MOpo3HjibHoft KaMepe xojiQHHjibHHK. Οροκ coxpaHeHHH - 1 Mecan e MOMeHTa pa3BeaeHna nPHaPe PHMOJIAH. ana nocTaHOBKH headKHHH y nNaNaNe 6pyT 2 προΟκρκΗ κροΒΗ: oaHa - c aHTHKoaryjihhtom hjih BbiaejieHHH JieHKomrroB, apiris - 6e3 cTa6njiH3aT0pa, & πη nojiyHeHHa ηητηβηοη chbopotkh.

KpoBb c aHTHKoaryjihhtom noMeinaiOT b τερΜοετΒτ npn TeMnepaType 37 ° C run noJiyHeHna nna3Mbi co B3BemeHHbiMH b κεη ηεΗκοηητΒΜΗ. IIna3My neHTpi ^ yrHpyK > T b τεηεΗκε 7-10 μηη npn 1500 ο6 / μηη. K ocaaicy, coaepMcameMy κηετκΗ JieftKOiiHTapHoro, ao6aBJiaiOT 3 m pacTBopa χηορπςτοΓο aMMOHHa hjih JiH3HpoeaHHa ΗΜειοιηηχεβ b ocajnce ΒρητροηκτοΒ. The yeast ycKopeHHa nponecca taH3Hca npo6npKy CTaeaT npn + 4 ° C If 10-15 μηη οτηβητ b xojioaHJibHHX. ΙΟιετχΗ aBaacab OTMbiea cpeaoft 199. IIojīyHeHHbiH ocaaoK cycneHaHpyioT b 1 mji 199 cpeabi npoBoaaT ποΛΟΗετ κπετοκ b KaMepe TopaeBa, nnojib3ya b KanecTse KpacnTejia MeTHjieHOBHH chhhh. Οηητηιοτ b 5 6oJibniHX KBaapaTax (N - hhcjio πολοηητηηηηχ κπετοκ). Hcxoaa H3 nojiyHeHHoro 3 EN 12487 pe3yjibTara γοτοβητ JieHKOUHTapHyio B3Becb H3 pacneTa 1 mjih κπετοκ b 1 mji. (N x 62.5: 10,000). 1-H npo6npKa - (b & ibbjichhc creneHH ceHCH6HiH3aiiHH κ ΡΗΜΟϋΑΗΥ): 6epyT 0.3 mJ 199 cpeab, α6aBJIOT 0.1 mJ of headKTHBa PHMOJ1AH h 0.1 b3B6ch jieftCompoundBB; 2-a npoČHpKa - BbiHBJieHHe HanHHHH 6jioKHpyiomHx ΒΗΤΗτεπ b ayTOCbroopoTKe 6epeMeHHoft b npncyTCTBHH PHMODIAHA:

6epyT 0.2 mji 199 cpenbi, noGaBJunoT 0.1 mJKKBBa PHMOJIAH, 0.1 mJ B3BecH

JieflKOIIHTOB H 0.1 MJI CbIBOpOTKH ΚρΟΒΗ δερβΜβΗΗΟΗ.

BcTpaxHBaiOT. CoflepacHMoe προβκροκ paBHOMepHO pacnpenenaioT. ΠροδκρκΗ noMemaiOT b TepMOCTaT πρκ 37 ° C Ha 2 naca. riocjie HHKy6aiiHH H3 KaacaoH προδκρκΗ 6epyT 0.1 mji, jio6aBJWK > T 0.4 mi KpacHTejia η προΒΟΛΗΤ ποΛΟΗβτ HenpHjninmHx κπετοκ bo Bcex 6ojibiirax KBaapaTax KaMepbi ae opaeBa. B nepBoft προδκρκε orthopedic CTeneHb ceHCH6HJiH3amiH neihoururBB 6epeMeHHOž κ npenapaiy PHMOJIAH; BO BTOpOft προβκρκβ ΟΠρβΛβΙΜΙΟΤ ΗΒΠΗΗΗβ 6jIOKHpyiOIIIHX CeHCH6HJIH3aiIHIO aHTHTeji b ayTocbmopoTKe 6epeMeHHoft πρκ noGaBneHHH b peaKiimo technology 'PHMOJIAH h onpeflejuiioT pa3HHiiy noKa3aTeneft creneHH ceHCH6HJiH3aiļHH h KOJinnecTsa 6jioKHpyiomHX aHTHTeji of φopMyπe: P = C-B, C RNE - noKa3aTejib ceHCH6HJiH3aiiHH JieHKomrroB GepeMeHHoft κ npenapaiy PHMOJIAH;

Ba - noKa3arejib ηηπηηηπ b cbiBopoTKe GepeMeHHofi 6jOKHpyioiiiHX aHTHTyJs πρκ aoSaBJieHHH b peaKiHio npApHaP PHMOJIAH; P - pa3HHiia noKa3aTyYe, η πρκ noKa3aThe C BH cBH 30 η MeHee η noKa3aTejie P paBHOM - 10 h MeHee (-15; -20 ht. Λ.), - y 6epeMeHHoft HHarHOCTHpyeTcst ra6ejib njiofla.

Cnoco6 OCHOB8H Ha npeKpameHHH alpha3HH (npHJiHnaHHa) JieŽKOHHTOB κ CTeKJIHHHbIM HJIH nJiaCTHKOBbLM ΠΟΒβρΧΗΟΟΤΗΜ B npHCyTCTBHH ΟΠείΙΗφΗΗεΟΚΟΓΟ aHTHreHa. On the other hand, we have the following: HHicy6aiiHH co ΟΠβΗΗφΗΗβΟΚΗΜ ΗΗΤΗΓβΗΟΜHJIH BblHBJieHHH K HeMy CeHCH6HJIH3aiIHH JieHKOIIHTOB naiHeHTa. B Kanecroe aHTHreHa Hcnojib30BaH npenapaT PHMOJIAH ..

Cnoco6 no3BOJi »eT nocroBepHO jmarHOCTHpoBaTb 3aMepinyio 6epeMeHHocTb (Missed abortion) - rnGejib nnona y SepeMeHHOH Ha cpoKax GepeMeHHOCTH 3-6 Henenb. 4

Kjrara “i®ciKiHie mpmMepM lipnnMep L BepeMeHHaa Γ-Ma. JI., B03pacT 29 JieT. B aHaMHe3e 1 6epeMeHH0CTi >, 3aKOHHHBHiaaca Φη3ηοπογηη € οκημη ponaMH h poacaeHHeM 3λοροβογο peSeHKa. Πρκ HacToameH 6epeMeHH0CTH o6paTHJiacB b naOoparopHio πρκ cpoKe 3 Hein e c acajio6aMH Ha THHyiime 6ojih b o6jiacTH Manai.

Pe3yjiī > TaTH ηοβογο cnoco6a ληβγηοοτηκη niGejiH πλολλ; noica3aTeab Ba paBeH 39. Fasting Meamy noica3aTheyHMH C h Ba cocTaBHJia: 29-39 = - 10. 3aKJIioMeHHe: 6epeMeHHOCTb He pa3BHBaerca (Missed abortion). € θΗθΓρ3φΗΗεοκοβ HCCJie / OBHH πΛΤΒΛΤΒΛΤΒΛΗΠΛΗΠΛΗΠ ra6ejib πλολλ πρκ εροκε 6 Henenb. IIpoBeaeHa doesn't take Abrasio cavi uteri. IIpiiMep 2. BepeMeHHaa 3-E T. B03pacT 32 ro ^ a. B aHaMHe3e: 1-a 6epeMeHHOcTb, 3aKOHHHBmaaca φΗ3Η0Π0ΓΗΗβααΐΜΗ po, aaMH h poacAeHHeM 3 ^ opoBoro peSemca; 2 -a δβρβΜβΗΗοσπ. - apTHm ^ HJibHbrit a6opT; 3 - 6epeMeHHOCTb - apTHU1HJibHbm abop-r; HaSjnoaaeMa 6epeMeHHOCTb 4-a. Πρκ nacToameS SepeMeHHOCTH o6pamjiacb b Jia6opaTopmo πρκ οροκε 3-4 He ^ ejiH c acajioOaMH Ha TaHyipHe 6ολη b oGnacra MaTKH h noacHHHHO-KpecnoBOM OT ^ E03B0H0HHHKK.

Pe3yjibTaTbi ηοβογο cnocoēa ληβγηοοτηκη, προβολημογο c πομοιπμο npenaoaTa PHMOJIAH: 4 HenejH 6epeMeHHOCTH - noKa3arejib C paBeH 30; nonasaTejib Ba paBeH 48. Pa3HiIl Meacny Ch Ba cocTaBHjia: 30-48 = -18.

SaKJHOHeHHe: OepeMeHHOCTi. not pa3BHBaeTca (Missed abortion).

Onepanna Abrasio cavi uteri πρκ cpoKe SepeMeHHoc ™ 5-6 Henejib.

HpiHiMep 3. BepeMeHHaa K-ea T., B03pacT 40 JieT. B aHaMHe3e: 1-a 6epeMeHHOCTb - npeayteBpeMeHHbiex οολμ πρκ οροκε 6epeMeHH0CTH 29 Henejib. Ρεβεκοκ nepes 5 nac yMep. 2-a SepeMeHHOCTb - cponHbie ροΛΜ, οληλκο, pe6eno yMep nepe3 4 naca nocjie poacneHHa.

HaejiaeMaa SepeMeHHOCTb 3-a. Πρκ nacToainež 6epeMeHHOCTH oSpanuiacb b jiaSopaTopnio πρκ οροκε 4-5 nenejib. c acajio6aMH Ha KpoBaHHCTbie BbmeaeHHa H3 ρολοββιχ nyreH.

Pe3yjibTaTM ηοβογο cnoco6a ληβγηοοτηκη, προβολημογο c πομοπιβκ) npenapaTa PHMOJIAH: 5-a nonsense SepeMeHHOCTH - noKa3aTejib C pasen -54; noicaaaTeab Ba pasen - 36, pasHHna Meacny C h Ba cocTasHjia: 54-36 = +18.

SaKJHOHenHe: SepeMeHHOCTb pa3BHBaeTca. ΗεοβχοΛΗΜ ληηβμηηημη κοκτροηΒ η jieneHHe β cTanHonape. Ep Λ ^ ιίΒΗεΗίπεΜ 6epeMeHHaa Ha6a ^ anacb eaceMecanHo.

Claims (1)

The Missed Abortion Diagnostic Method for Pregnant Woman, Blood Testing, describes the production of a leukocyte suspension from the blood of a pregnant woman at a cost of 10 million. lml and native serum of pregnant blood, take 2 tubes: determine the degree of sensitization of a pregnant woman to leukocytes to RIMOLANS in the first test, which contains the chorionic gonadotropin, progesterone, prolactin, estradiol, luteinizing hormone, the second tube determines the sensitization of anti-germs in pregnant women with the addition of RIMOLANS. and determines the difference between the degree of sensitization and the number of blocking anti-cells according to the formula: P = S - Ba, where: S is the maternal leukocyte sensitization index against RIMOLANS; Ba - an indicator of the presence of blocking antibodies in the serum of a pregnant woman with a response preparation RIMOLANS; P - difference of indicators; and if S is equal to 30 and less, and P is '10 'and less (-15; -20, etc.) then a pregnant woman is diagnosed with a dead fetus.