LU502945B1 - Efficient preparation method of active substances in sanghuangporus vaninii - Google Patents

Efficient preparation method of active substances in sanghuangporus vaninii Download PDF

Info

Publication number
LU502945B1
LU502945B1 LU502945A LU502945A LU502945B1 LU 502945 B1 LU502945 B1 LU 502945B1 LU 502945 A LU502945 A LU 502945A LU 502945 A LU502945 A LU 502945A LU 502945 B1 LU502945 B1 LU 502945B1
Authority
LU
Luxembourg
Prior art keywords
vaninii
sanghuangporus
polysaccharide
polyphenol
active substances
Prior art date
Application number
LU502945A
Other languages
German (de)
Inventor
Guoying Lv
Zuofa Zhang
Original Assignee
Zhejiang Acad Agricultural Sci
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Zhejiang Acad Agricultural Sci filed Critical Zhejiang Acad Agricultural Sci
Priority to LU502945A priority Critical patent/LU502945B1/en
Application granted granted Critical
Publication of LU502945B1 publication Critical patent/LU502945B1/en

Links

Classifications

    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/105—Plant extracts, their artificial duplicates or their derivatives
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/06—Fungi, e.g. yeasts
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Mycology (AREA)
  • Botany (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Animal Behavior & Ethology (AREA)
  • Medical Informatics (AREA)
  • Alternative & Traditional Medicine (AREA)
  • Microbiology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Nutrition Science (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)

Abstract

The invention discloses an efficient preparation method of active substances in Sanghuangporus vaninii, which comprises S1. pretreatment of raw materials: drying and crushing Sanghuangporus vaninii fruiting bodies to form Sanghuangporus vaninii powder; S2, treating MgO and tetrahydrofuran: adding 40-60% MgO and 20-40 times tetrahydrofuran in Sanghuangporus vaninii powder, uniformly stirring, placing in a reaction kettle, and extracting at 200-260℃ for 30-60 min; S3, ultrasonic treatment: after the reaction is finished, cooling to room temperature, adding water 1-2 times the volume of the mixture, performing ultrasonic treatment at 30-50℃ for 10-30 min, and filtering the mixture to obtain filtrate containing Sanghuangporus vaninii polyphenol and Sanghuangporus vaninii polysaccharide. The filtrate can be further separated to obtain Sanghuangporus vaninii polyphenol and Sanghuangporus vaninii polysaccharide respectively. The method can prepare Sanghuangporus vaninii polyphenol and Sanghuangporus vaninii polysaccharide at the same time, improves the extraction rate of Sanghuangporus vaninii polysaccharide and Sanghuangporus vaninii polyphenol, has simple process and is suitable for large-scale production.

Description

EFFICIENT PREPARATION METHOD OF ACTIVE SUBSTANCES IN 502945
SANGHUANGPORUS VANINII
The invention relates to the field of edible and medicinal mushroom processing, in particular to an efficient preparation method of active substances in Sanghuangporus vaninii.
BACKGROUND
Sanghuangporus vaninii is a kind of precious edible and medicinal fungus, and has the reputation of "forest gold". As a traditional Chinese herbal medicine,
Sanghuangporus vaninii is first recorded in Shennong Herbal Classic and Li Shizhen's
Compendium of Materia Medica. It is mainly used to stop diarrhea, metrorrhagia and leukorrhagia, diarrhea due to spleen deficiency, and it can also benefit the five internal organs, detoxify and promote blood circulation. According to research statistics,
Sanghuangporus vaninii has more than 20 pharmacological functions, including antibacterial, anti-inflammatory, antioxidant, anti-tumor, enhancing immunity, protecting liver, lowering blood sugar, reducing blood lipid and resisting pneumonia.
Generally, edible fungi can't achieve the therapeutic effect when eaten directly. Only by extracting, separating and purifying the effective ingredients can the efficacy be improved and pharmacological effects be exerted. In the preparation of active ingredients, extraction is the first and most critical step. How to release the active ingredients to the maximum extent is of great significance.
China patent document CN108610391A discloses a method for extracting polysaccharide and adenosine from Sanghuangporus vaninii fruiting body, which comprises the following steps: (1) pulverizing Sanghuangporus vaninii fruiting body into Sanghuangporus vaninii fruiting body particles, and putting them into an extraction tank; (2) adding water 6-10 times the volume of Sanghuangporus vaninii fruiting body particles into the extraction tank, continuously refluxing for 1.5-3 hours, performing suction filtration, continuously refluxing the filter residue with water 4-5 times the volume of the filter residue for 2-3 hours, and performing suction filtration; (3) mixing filtrates obtained by two times of suction filtration in step (2), and concentrating to obtain 0.3-0.5 g/mL Sanghuangporus vaninii solution; (4) adjusting the pH value of Sanghuangporus vaninii solution in step (3) to 9-10 with sodium 17008965 hydroxide solution, loading the Sanghuangporus vaninii solution with adjusted pH value into a stainless steel chromatographic column filled with macroporous resin, and controlling the flow rate of effluent to obtain crude Sanghuangporus vaninii polysaccharide solution; (5) adjusting the pH of the crude Sanghuangporus vaninii polysaccharide solution in step (4) to neutral with sulfuric acid solution, passing through a first ultrafiltration membrane which can filter out macromolecules such as protein, collecting filtrate, and passing through a second ultrafiltration membrane which removes micromolecule impurities, and obtaining Sanghuangporus vaninii polysaccharide by vacuum concentration of the obtained retentate; (6) eluting the adsorbate adsorbed in the macroporous resin in step (4) with ethanol solution, and concentrating the obtained eluent under reduced pressure and vacuum to obtain extract; (7) take the extract from step (6) and silica gel according to the mass ratio of 1:12, dry mix the sample, apply it to a chromatography column, elute with 1-2 column volumes of ethyl acetate, then elute with a mixed eluent of ethyl acetate and acetone, collect one fraction every 1,500 mL, perform liquid phase detection, combine the 24th-29th fractions in the fractions, concentrate under reduced pressure and vacuum, combine the precipitates, and filter. The filtered precipitate is mixed with the original precipitate, and the mixed precipitate is recrystallized in acetone-ethanol solution with the volume ratio of 6:4 to obtain colorless needle-shaped crystalline adenosine, which is filtered and dried in a vacuum dryer at 50°C to obtain the finished adenosine product.
China patent document CN108542922A discloses an efficient preparation method of active ingredients in Sanghuangporus vaninii, which includes the following steps: (1) pretreatment of raw materials: (1) drying Sanghuangporus vaninii fruiting body at 50-60°C, pulverizing, and sieving with 40-60 mesh sieve to obtain Sanghuangporus vaninii powder; (2) mixing Sanghuangporus vaninii powder obtained in step one with deionized water according to the mass-volume ratio of 1: 20-1: 50, adding cellulase accounting for 1%-2% of the mass of Sanghuangporus vaninii powder after uniform mixing, and treating at 45-55°C for 1-2 hours to obtain a reaction mixture; (3) adding 502945 the reaction mixture obtained in the second step into an extraction kettle for extraction, where the pressure in the extraction kettle is 5-20 MPa, the extraction temperature is 100-200°C, and the extraction time is 30-90 min, and then obtaining the extraction liquid after the extraction; (4) centrifuging the extract obtained in step three at a rotating speed of 4,500-7,000 r/min for 10-20 min, and separating the supernatant to obtain the first supernatant; (5) adding cetylpyridinium chloride into the first supernatant, shaking well, standing at room temperature for 24h-36h, and centrifuging at a rotating speed of 3,500-7,000 r/min for 10-15min to obtain the first precipitate and the second supernatant; (6) adding the first precipitate obtained in step (5) into the ethanol solution of NaCl to obtain a precipitate mixed solution, shaking the precipitate mixed solution in a shaking table for 1-1.5 h, centrifuging at a rotating speed of 3,500-7,000 r/min for 10-15 min, centrifuging to obtain a precipitate, washing the precipitate with 80% ethanol, and freeze-drying to obtain high-activity
Sanghuangporus vaninii polysaccharide SP1; (7) The second supernatant obtained in step 5 is concentrated under reduced pressure and freeze-dried to obtain an extract rich in Sanghuangporus vaninii triterpenes and flavonoids.
China patent document CN106491662A discloses a method for simultaneously extracting polysaccharides and flavonoids from Sanghuangporus vaninii cultivation waste, which comprises the following steps: (1) drying and crushing Sanghuangporus vaninii cultivation waste to obtain dry powder of Sanghuangporus vaninii cultivation waste; (2) mixing dry powder of Sanghuangporus vaninii cultivation waste with alkaline ethanol solution according to the mass ratio of 1:10-1:30; (3) performing ultrasonic treatment on the mixed solution in step (2) for 20-40 min, and filtering to obtain filtrate and residue; (4) adding deionized water which is 8-12 times the weight of the residue into the residue in step (3), uniformly mixing, adjusting the pH value to 4-6 to obtain a mixed solution, adding a compound enzyme which accounts for 0.2-0.5% of the weight of the mixed solution, where the compound enzyme is composed of cellulase and glucose oxidase, performing enzymolysis at 45-55°C for 40-80 min, and centrifuging the enzymolysis solution to obtain supernatant and precipitate; the compound enzyme is a mixture of cellulase: glucose oxidase = 2-3: 1; 502945 (5) the filtrate obtained in step (3) is subjected to rotary evaporation, ethanol is removed, ethyl acetate is added for extraction and liquid separation, and the upper layer liquid is concentrated and dried under reduced pressure to obtain
Sanghuangporus vaninii flavone; (6) the supernatant obtained in the step (4) is concentrated under reduced pressure by a rotary evaporator, then subjected to alcohol precipitation and centrifugation to obtain supernatant and sediment, and the sediment is Sanghuangporus vaninii polysaccharide.
There is no literature report on simultaneous extraction of Sanghuangporus vaninii polyphenol and Sanghuangporus vaninii polysaccharide in the prior art.
SUMMARY
According to the inventor's analysis, this is because Sanghuangporus vaninii fruiting body has a high degree of lignification and a complex structure, and the active substances are generally combined with lignin through physical or chemical bonds, so it is difficult to extract the effective components of Sanghuangporus vaninii polyphenol and Sanghuangporus vaninii polysaccharide by common methods at the same time. Therefore, it is also an effective extraction method to destroy the bond between active substances and lignin by removing lignin.
In view of this, the purpose of the present invention is to provide an efficient preparation method of active substances in Sanghuangporus vaninii, which can efficiently extract Sanghuangporus vaninii polyphenols and Sanghuangporus vaninii polysaccharides at the same time, so that the effective ingredients Sanghuangporus vaninii flavonoids and Sanghuangporus vaninii polysaccharides can be released at the same time to the maximum extent.
The adopted technical scheme is as follows:
An efficient preparation method of active substances in Sanghuangporus vaninii comprises the following steps:
S1, pretreatment of raw materials: drying and crushing Sanghuangporus vaninii fruiting bodies to form Sanghuangporus vaninii powder;
S2, treating MgO and tetrahydrofuran: adding 40-60% MgO and 20-40 times 502945 tetrahydrofuran in Sanghuangporus vaninii powder, uniformly stirring, placing in a reaction kettle, and extracting at 200-260°C for 30-60 min;
S3, ultrasonic treatment: after the reaction is finished, cooling to room temperature, 5 adding water 1-2 times the volume of the mixture, performing ultrasonic treatment at 30-50°C for 10-30 min, and filtering the mixture to obtain filtrate containing
Sanghuangporus vaninii polyphenol and Sanghuangporus vaninii polysaccharide.
In the technical scheme, on the one hand, tetrahydrofuran has low toxicity, and MgO is used as a catalyst, which can dissolve lignin in Sanghuangporus vaninii fruiting body under the action of MgO, so that more Sanghuangporus vaninii flavonoids and
Sanghuangporus vaninii polysaccharides are released, thereby improving the extraction rate.
On the other hand, tetrahydrofuran dissolves lignin of Sanghuangporus vaninii fruiting body under the catalysis of MgO, and then ultrasonic treatment is carried out to release effective components Sanghuangporus vaninii flavonoids and
Sanghuangporus vaninii polysaccharides to the greatest extent at the same time.
Further, S4, adding equal volume of dichloromethane into the filtrate for extraction, and drying the organic layer to obtain Sanghuangporus vaninii polyphenol. The yield of Sanghuangporus vaninii polyphenol is 2%-4.7%.
Further, S5, after the water layer is concentrated, absolute ethanol is added to make the ethanol concentration reach 70-80% by volume, and the precipitate is collected to obtain Sanghuangporus vaninii polysaccharide. The yield of Sanghuangporus vaninii polysaccharide is 8.7%-11.2%.
Furthermore, in S2, MgO accounting for 50% of the weight of Sanghuangporus vaninii powder and tetrahydrofuran accounting for 20-40 times of the weight of
Sanghuangporus vaninii powder are added into Sanghuangporus vaninii powder.
Further, in S5, after the water layer is concentrated, absolute ethanol is added to make the ethanol concentration reach 75% by volume, and the precipitate is collected to obtain Sanghuangporus vaninii polysaccharide.
Further, in S1, Sanghuangporus vaninii fruiting body is dried at 50-60°C and then 17008965 micronized.
Similarly, from the product point of view, the present invention can also provide a mixed solution containing Sanghuangporus vaninii polyphenol and Sanghuangporus vaninii polysaccharide, which is prepared by the efficient preparation method described in the above scheme.
Furthermore, equal volume of dichloromethane is added to the mixed solution for extraction, and the organic layer is dried to obtain Sanghuangporus vaninii polyphenol, and the yield of Sanghuangporus vaninii polyphenol is 2%-4.7%.
Furthermore, after the water layer is concentrated, absolute ethanol is added to make the ethanol concentration reach 70-80% by volume, and Sanghuangporus vaninii polysaccharide is obtained by collecting and precipitating, and the yield of
Sanghuangporus vaninii polysaccharide is 8.7-11.2%.
The invention has the advantages that: the efficient preparation method of active substances in Sanghuangporus vaninii can prepare Sanghuangporus vaninii polyphenol and Sanghuangporus vaninii polysaccharide at the same time, improve the extraction rate of Sanghuangporus vaninii polysaccharide and Sanghuangporus vaninii polyphenol, and has simple process and is suitable for large-scale production.
DESCRIPTION OF THE INVENTION
The present invention will be described in detail by the following specific embodiments, but the use and purpose of these exemplary embodiments are only for exemplifying the present invention, and do not constitute any limitation on the actual protection scope of the present invention, let alone limit the protection scope of the present invention.
Embodiment 1
An efficient preparation method of active substances in Sanghuangporus vaninii comprises the following steps:
S1, pretreatment of raw materials: drying and crushing Sanghuangporus vaninii fruiting bodies;
S2, treating MgO and tetrahydrofuran: adding 50% of MgO and 20 times of 502945 tetrahydrofuran into Sanghuangporus vaninii powder, uniformly stirring, placing in a reaction kettle, and extracting at 200°C for 30 min;
S3, after the reaction is finished and cooled to room temperature, the mixture is added with water which is 1 time of its volume, subjected to ultrasonic treatment at 30°C for min, and the mixture is filtered;
S4, adding equal volume of dichloromethane into the filtrate for extraction, and drying the organic layer to obtain Sanghuangporus vaninii polyphenol with a yield of 2.1%; 10 SS, after the water layer is concentrated, absolute ethanol is added to make the ethanol concentration reach 75% by volume, and the precipitate is collected to obtain
Sanghuangporus vaninii polysaccharide. The yield of Sanghuangporus vaninii polysaccharide is 8.9%.
Embodiment 2
An efficient preparation method of active substances in Sanghuangporus vaninii comprises the following steps:
S1, pretreatment of raw materials: drying and crushing Sanghuangporus vaninii fruiting bodies;
S2, treating MgO and tetrahydrofuran: adding 50% of MgO and 30 times of tetrahydrofuran into Sanghuangporus vaninii powder, uniformly stirring, placing in a reaction kettle, and extracting at 220°C for 40 min;
D, after the reaction is finished and cooled to room temperature, the mixture is added with 1.5 times of its volume of water, subjected to ultrasonic treatment at 40°C for 20 min, and the mixture is filtered,
E, adding equal volume of dichloromethane into the filtrate for extraction, and drying the organic layer to obtain Sanghuangporus vaninii polyphenol with a yield of 2.65%.
F, after the water layer is concentrated, absolute ethanol is added to make the ethanol concentration reach 75% by volume, and the Sanghuangporus vaninii polysaccharide is obtained by collecting the precipitate. The yield of Sanghuangporus vaninii polysaccharide is 9.47%.
Embodiment 3 502945
An efficient preparation method of active substances in Sanghuangporus vaninii comprises the following steps:
S1, pretreatment of raw materials: drying and crushing Sanghuangporus vaninii fruiting bodies;
S2, treating MgO and tetrahydrofuran: adding 50% of MgO and 40 times of tetrahydrofuran into Sanghuangporus vaninii powder, uniformly stirring, placing in a reaction kettle, and extracting at 240°C for 50 min;
S3, after the reaction is finished and cooled to room temperature, the mixture is added with 1.8 times of its volume of water, subjected to ultrasonic treatment at 45°C for 25 min, and the mixture is filtered,
S4, adding equal volume of dichloromethane into the filtrate for extraction, and drying the organic layer to obtain Sanghuangporus vaninii polyphenol with a yield of 3.46%;
S5, after the water layer is concentrated, absolute ethanol is added to make the ethanol concentration reach 75% by volume, and the precipitate is collected to obtain
Sanghuangporus vaninii polysaccharide. The yield of Sanghuangporus vaninii polysaccharide is 10.05%.
Embodiment 4
An efficient preparation method of active substances in Sanghuangporus vaninii comprises the following steps:
S1, pretreatment of raw materials: drying Sanghuangporus vaninii fruiting body at 55°C, and micronizing;
S2, treating MgO and tetrahydrofuran: adding 50% of MgO and 35 times of tetrahydrofuran into Sanghuangporus vaninii powder, uniformly stirring, placing in a reaction kettle, and extracting at 250°C for 60 min;
S3, after the reaction is finished, cooling to room temperature, adding water twice the volume of the mixture, performing ultrasonic treatment at 50°C for 30 min, and filtering the mixture;
S4, adding equal volume of dichloromethane into the filtrate for extraction, and 502945 drying the organic layer to obtain Sanghuangporus vaninii polyphenol with a yield of 3.89%.
SS, after the water layer is concentrated, absolute ethanol is added to make the ethanol concentration reach 75% by volume, and the precipitate is collected to obtain
Sanghuangporus vaninii polysaccharide. The yield of Sanghuangporus vaninii polysaccharide is 10.54%.
Embodiment 5
An efficient preparation method of active substances in Sanghuangporus vaninii comprises the following steps:
S1, pretreatment of raw materials: drying and crushing Sanghuangporus vaninii fruiting bodies;
S2, treating MgO and tetrahydrofuran: adding 50% of MgO and 40 times of tetrahydrofuran into Sanghuangporus vaninii powder, uniformly stirring, placing in a reaction kettle, and extracting at 260°C for 50 min;
S3, after the reaction is finished and cooled to room temperature, the mixture is added with 1.6 times of its volume of water, subjected to ultrasonic treatment at 45°C for 25 min, and the mixture is filtered,
S4, adding equal volume of dichloromethane into the filtrate for extraction, and drying the organic layer to obtain Sanghuangporus vaninii polyphenol with a yield of 3.55%;
SS, after the water layer is concentrated, absolute ethanol is added to make the ethanol concentration reach 75% by volume, and the precipitate is collected to obtain
Sanghuangporus vaninii polysaccharide. The yield of Sanghuangporus vaninii polysaccharide is 9.98%.
The series of detailed descriptions listed above are only specific descriptions of the feasible embodiments of the present invention, and they are not intended to limit the scope of protection of the present invention. All equivalent embodiments or changes that do not depart from the technical spirit of the present invention should be included in the scope of protection of the present invention.

Claims (9)

CLAIMS LU502945
1. An efficient preparation method of active substances in Sanghuangporus vaninii, characterized by comprising the following steps: S1, pretreatment of raw materials: drying and crushing Sanghuangporus vaninii fruiting bodies to form Sanghuangporus vaninii powder, S2, treating MgO and tetrahydrofuran: adding 40-60% MgO and 20-40 times tetrahydrofuran in Sanghuangporus vaninii powder, uniformly stirring, placing in a reaction kettle, and extracting at 200-260°C for 30-60 min; S3, ultrasonic treatment: after the reaction is finished, cooling to room temperature, adding water 1-2 times the volume of the mixture, performing ultrasonic treatment at 30-50°C for 10-30 min, and filtering the mixture to obtain filtrate containing Sanghuangporus vaninii polyphenol and Sanghuangporus vaninii polysaccharide.
2. The efficient preparation method of active substances in Sanghuangporus vaninii according to claim 1, characterized by further comprising S4: the filtrate is added with equal volume of dichloromethane for extraction, and the organic layer is dried to obtain Sanghuangporus vaninii polyphenol.
3. The efficient preparation method of active substances in Sanghuangporus vaninii according to claim 2, characterized by further comprising SS: after the water layer is concentrated, absolute ethanol is added to make the ethanol concentration reach 70-80% by volume, and the precipitate is collected to obtain Sanghuangporus vaninii polysaccharide.
4. The efficient preparation method of active substances in Sanghuangporus vaninii according to claim 3, characterized in that in S2, MgO accounting for 50% of the weight of Sanghuangporus vaninii powder and tetrahydrofuran accounting for 20-40 times of the weight of Sanghuangporus vaninii powder are added.
5. The efficient preparation method of active substances in Sanghuangporus vaninii according to claim 3, characterized in that in S5, after the water layer is concentrated, absolute ethanol is added to make the ethanol concentration reach 75% by volume, and the precipitate is collected to obtain Sanghuangporus vaninii polysaccharide.
6. The efficient preparation method of active substances in Sanghuangporus vaninii 17008965 according to claim 3, characterized in that in S1, Sanghuangporus vaninii fruiting body is dried at 50-60°C and then micronized.
7. A mixed solution containing Sanghuangporus vaninii polyphenol and Sanghuangporus vaninii polysaccharide, characterized in that it is prepared by the efficient preparation method of claim 1.
8. The mixed solution containing Sanghuangporus vaninii polyphenol and Sanghuangporus vaninii polysaccharide according to claim 7, characterized in that equal volume of dichloromethane is added for extraction, and the organic layer is dried to obtain Sanghuangporus vaninii polyphenol, and the yield of Sanghuangporus vaninii polyphenol is 2%-4.7%.
9. The mixed solution containing Sanghuangporus vaninii polyphenol and Sanghuangporus vaninii polysaccharide according to claim 7, characterized in that after the water layer is concentrated, absolute ethanol is added to make the ethanol concentration reach 70-80% by volume, and the Sanghuangporus vaninii polysaccharide is obtained by collecting the precipitate, and the yield of Sanghuangporus vaninii polysaccharide is 8.7-11.2%.
LU502945A 2022-10-22 2022-10-22 Efficient preparation method of active substances in sanghuangporus vaninii LU502945B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
LU502945A LU502945B1 (en) 2022-10-22 2022-10-22 Efficient preparation method of active substances in sanghuangporus vaninii

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
LU502945A LU502945B1 (en) 2022-10-22 2022-10-22 Efficient preparation method of active substances in sanghuangporus vaninii

Publications (1)

Publication Number Publication Date
LU502945B1 true LU502945B1 (en) 2023-04-24

Family

ID=86097379

Family Applications (1)

Application Number Title Priority Date Filing Date
LU502945A LU502945B1 (en) 2022-10-22 2022-10-22 Efficient preparation method of active substances in sanghuangporus vaninii

Country Status (1)

Country Link
LU (1) LU502945B1 (en)

Similar Documents

Publication Publication Date Title
WO2020177420A1 (en) Ultrasonic composite acidic water extraction method for cordyceps polysaccharide and cordycepin in cordyceps militaris
CN101973984B (en) Method for extracting general flavones from bamboo leaves
CN110201012B (en) Preparation method and application of purslane extract
CN110882285A (en) Efficient preparation method of active substances in phellinus igniarius
CN101961371A (en) Method for extracting and separating ginsenoside, flavone and polysaccharide from sweet gynostemma pentaphylla
CN102718817A (en) Method for preparing anthocyanin extract from black bean peel
CN106810618A (en) A kind of extraction from Chinese caterpillar fungus culture medium and the method for continuous polysaccharide enrichment
CN101856427B (en) Method for extracting solanine from potato peels
CN111206063A (en) Preparation method of high-stability acylated black rice anthocyanin
CN105998103A (en) Chestnut flower activated extract and preparation method and application thereof
LU502945B1 (en) Efficient preparation method of active substances in sanghuangporus vaninii
CN112159451A (en) Gynostemma pentaphylla saponin extract and preparation method thereof
CN113024679B (en) Method for extracting selenium polysaccharide and polyphenol from selenium-rich moringa seeds
CN113913029B (en) Method for preparing effective components of gardenia jasminoides ellis
CN108126000B (en) Method for extracting and preparing panax notoginseng saponins from fresh panax notoginseng
CN107213180B (en) Separation and extraction method of notoginseng flavone
CN114107418B (en) Preparation method of ginseng polypeptide
CN115010618A (en) Separation and purification method of aureoyl amide alcohol ester capable of reducing uric acid and application thereof
CN116139055A (en) Preparation method and application of vitamin C-enriched roxburgh rose extract
CN113694152B (en) Method for obtaining coix seed extracting solution by high-stability enzymolysis method
CN110551777A (en) preparation method of aloe polysaccharide
CN113278089A (en) Separation, extraction and purification method of dogwood seed polysaccharide
CN114159487A (en) Method for extracting and purifying folium artemisiae argyi total flavonoids
CN113662976A (en) Extraction method of purslane extract and obtained product
CN102690359A (en) Method for extracting starch and cucurbitacins from siraitia grosvenorii roots

Legal Events

Date Code Title Description
FG Patent granted

Effective date: 20230424