LU502877B1

LU502877B1 - A Temperature Measuring Module for Digital PCR Microfluidic Chip

Info

Publication number: LU502877B1
Application number: LU502877A
Authority: LU
Inventors: Lili Cui; Jian'an He; Ran Zhang; Xi Chen; Dayong Gu
Original assignee: Shenzhen Int Travel Health Care Center Shenzhen Customs Port Outpatient Department; Shenzhen Second Peoples Hospital Shenzhen Inst Of Translational Medicine; Shenzhen Acad Inspection & Quarantine
Priority date: 2022-10-06
Filing date: 2022-10-06
Publication date: 2023-04-06

Abstract

The invention discloses a digital PCR microfluidic chip temperature measurement module, which relates to the technical field of microfluidic chip temperature measurement and comprises a placing box, wherein a chip plate is arranged in the placing box, a plurality of groups of grooves are formed in the top end of the chip plate, two ends of the top end of the chip plate are respectively provided with a sample adding hole, A detection assembly is arrange in that top end of the top cover and at the top end of the top cov, a plurality of L-shaped limiting plates are arrange at the outer side of the bottom end in the placing box, and two rotating shafts are arranged at the top end in the top cover through a rotating shaft According to the invention, the position of the chip plate is limited by using the limiting plate, the position of the chip plate is determined, and the heat insulation pad is pressed on the chip plate by pushing the mounting plate to move downwards, so that the chip board is pressed and fixed; and the temperature detection probe is inserted at a proper position, so that the Operation is convenient without using an extemal tool.

Description

Specification

LU502877

A Temperature Measuring Module for Digital PCR Microfluidic Chip

Technical field

The invention relates to the technical field of microfluidic chip temperature measurement, in particular to a digital PCR microfluidic chip temperature measurement module.

Background Technology

Digital PCR is a technique for absolute quantification of nucleic acid molecules. Compared with qPCR, digital PCR allows the experimenter to directly count the number of DNA molecules, which is an absolute quantification of the initial sample. Microfluidics is a technique for precise control and manipulation of microscale fluids, which has the characteristics of small capacity and low energy consumption. People use microfluidic technology to integrate sample preparation, reaction, separation, detection and other basic operation units of biological, chemical and medical analysis processes into a microfluidic chip with a microfluidic channel as a basic structure, and multi-point continuous temperature measurement is needed when related experiments of a digital PCR microfluidic chip are carried out;

After searching, the Chinese Patent Authorization Publication No.

CN215757374U, published on February 8, 2022, discloses a PCR microfluidic chip temperature measurement module, in which it is pointed out that "the bottom surface of the movable briquette abuts against the upper surface of the microfluidic chip, and the area of the bottom surface of the movable briquette covers the temperature sensor";

However, the movable block of the device needs to abut against the chip with 1

Specification

LU502877 the help of external tools, which is troublesome to operate and inaccurate in positioning. In view of this, in view of the above problems, in-depth research has been carried out, resulting in this case.

Summary of the invention

The purpose of the present invention is to provide a digital PCR microfluidic chip temperature measurement module, so as to solve the problems in the background art that the position of the plate abutting against the chip is difficult to determine and the abutting operation is inconvenient.

In order to achieve the purpose, the technical scheme provided by the invention is as follows: the digital PCR microfluidic chip temperature measurement module comprises a placing box, wherein a chip board is arranged in the placing box, a plurality of groups of grooves are formed in the top end of the chip board, sampling holes are arranged at two ends of the top end of the chip board, and a top cover is arranged at one end of the placing box through a hinged part. The top end of the top cover is internally provided with a detection assembly, the top end of the top cover is internally provided with a plurality of L-shaped limiting plates, the outer side of the bottom end in the placing box is provided with a plurality of L-shaped limiting plates, A plurality of temperature detection probes are arranged at the bottom ends of the mounting plates.

Preferably, the detection assembly comprises a display module, the display module is installed at the top end of the top cover, a cavity is formed inside the top end of the top cover, a control module is installed at one end of a bottom end inside the cavity, and a power supply module is arranged at the other end of the bottom end inside the cavity. 2

Specification

LU502877

Preferably, a driven bevel gear is fixed at the bottom end of the rotating shaft, a plurality of telescopic rods are mounted at the top end of each mounting plate, one end of each telescopic rod is connected with the top end inside the top cover, two groups of fixing blocks are fixed at the top end inside the top cover, two fixing blocks are arranged in each group, and rotating rods are mounted inside the two groups of fixing blocks through bearings. One end of each rotating rod extends to the outer side of the top cover and is provided with a knob, and the other end of each rotating rod extends to the upper part of the mounting plate and is provided with a driving bevel gear.

Preferably, the driving bevel gear and the driven bevel gear have the same size, and the driving bevel gear is engaged with the driven bevel gear.

Preferably, a heat insulation pad is adhered to the bottom end of the mounting plate, and one end of the temperature detection probe penetrates below the heat insulation pad.

Preferably, the outer wall of the rotating shaft is provided with an external thread, and the inner wall of the movable plate is provided with an internal thread matched with the external thread.

Preferably, two clamping boxes are fixed at one end of the placing box, clamping grooves are formed in two sides inside the clamping boxes, two mounting boxes are fixed at one end of the top cover, inserting blocks are slidably mounted inside the mounting boxes, the bottom ends of the inserting blocks extend into the clamping boxes, and moving strips are slidably mounted on two sides inside the inserting blocks. A clamping block is fix at that bottom end of one side of each moving bar, one end of each clam block extends to the inside of each 3

Specification

LU502877 clamping groove, a plurality of telescopic spring rod are mounted between one sides of the two moving bars, a poking block is fixed at one end of each moving bar, one ends of the poking blocks extend to one ends of the mounting boxes, and the bottoms of the mounting boxes and the clamping boxes are positioned on the same horizontal line.

Compared with the prior art, the invention has the following beneficial effects:

After the top cover is closed, the rotating shaft is caused to rotate so as to cause the movable plate to move downwards, the connecting rod is used for pushing the mounting plate to move downwards, the heat insulation pad is pressed on the chip board, a temperature detection probe is inserted into the top cover, multi-point temperature measurement is carried out on the chip, and the chip board is pressed and fixed, In addition, the insertion position of the temperature detection probe is appropriate, and the operation is convenient without the help of external tools.

Description of the drawings

Fig. 1 is a schematic plan view of the patent of the present invention in an open state;

Fig. 2 is a schematic diagram of the front sectional structure of the mounting box and the clamping box in the clamping state of the present invention;

Fig. 3 is a top view structure diagram of the patent of the present invention in the closed state;

Fig. 4 is a partial side sectional view of the top cover of the present invention.

In the figure: 1. Temperature detection probe; 2. Mounting box; 3. Rotating 4

Specification

LU502877 rod; 4. Fixed block; 5. Limit plate; 6. Sampling hole; 7. Clamping box; 8. Groove; 9. Chip board; 10. Placement box; 11. Heat insulation pad; 12. Top cover; 13.

Insertion block; 14. Slot; 15. Telescopic spring rod; 16. Clamping block; 17.

Moving strip; 18. Shifting block; 19. Cavity; 20. Control module; 21. Power module; 22. Display module; 23. Telescopic rod; 24. Driving bevel gear; 25.

Mounting plate; 26. Movable plate; 27. Driven bevel gear; 28. Rotating shaft; 29.

Connecting rod.

Description of the invention

In the following, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments, not all of the embodiments. Based on the embodiments in the patent of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative work belong to the scope of protection of the patent of the present invention.

In the embodiment, referring to figs. 1-4, a digital PCR microfluidic chip temperature measuring module comprises a digital PCR microfluidic chip temperature measuring module, which comprises a placing box 10, wherein a chip plate 9 is arranged in the placing box 10, a plurality of groups of grooves 8 are formed in the top end of the chip plate 9, and sample adding holes 6 are arranged at both ends of the top end of the chip plate 9. One end of the holding box 10 is mounted with a top cover 12 through a hinge;

Two clamping boxes 7 are fixed at one end of the placing box 10, clamping grooves 14 are arranged at two sides inside the clamping boxes 7, two mounting

Specification

LU502877 boxes 2 are fixed at one end of the top cover 12, inserting blocks 13 are arranged inside the mounting boxes 2 in a sliding manner, the bottom ends of the inserting blocks 13 extend to the inside of the clamping boxes 7, moving strips 17 are arranged at two sides inside the inserting blocks 13 in a sliding manner, À clamping block 16 is fixed at the bottom end of one side of each moving bar 17, one end of each clamping block 16 extends to the inside of the clamping groove 14, a plurality of telescopic spring rods 15 are installed between one sides of the two moving bars 17, a shifting block 18 is fixed at one end of each moving bar 17, and one end of the shifting block 18 extends to one side of the mounting box 2. The bottom ends of the mounting box 2 and the clamping box 7 are positioned on the same horizontal line;

Specifically, as shown in fig. 1 and fig. 2, the top cover 12 is fastened, and then the two shifting blocks 18 are pulled inward to make the moving bars 17 close to pull the clamping block 16 into the inserting block 13, and then the inserting block 13 is inserted into the clamping box 7 and the shifting blocks 18 are loosened, and the telescopic spring rod 15 pushes the moving bars 17 outward to make the clamping block 16 inserted into the clamping groove 14. The connection between the mounting box 2 and the clamping box 7 is completed, and the top cover 12 is quickly closed.

A detection assembly is arrange inside and at that top end of the top cover 12;

The detection assembly comprises a display module 22, the display module 22 is mounted on the top end of the top cover 12, a cavity 19 is formed in the top end of the top cover 12, a control module 20 is mounted at one end of a bottom end inside the cavity 19, and a power module 21 is mounted at the other end of the 6

Specification

LU502877 bottom end inside the cavity 19;

The control module 20, the display module 22, and the temperature detection probe 1 are all electrically connected to the power module 21 through wires, and are powered by the power module 21. The temperature detection probe 1 is electrically connected to a single chip microcomputer inside the control module 20, and is controlled by the control module 20.

Specifically, as shown in fig. 1 and fig. 3, the temperature detection probe 1 measures the temperature of the chip at multiple points, and the control module 20 records the measured temperature and displays it on the display module 22.

A plurality of L-shaped limiting plates 5 are mounted on the outer side of the bottom end inside the placing box 10, two rotating shafts 28 are mounted on the top end inside the top cover 12 through a rotating shaft, a movable plate 26 is sleeved on the outer wall of each rotating shaft 28, a plurality of connecting rods 29 are mounted at the bottom ends of the movable plates 26, and a mounting plate is fixed at the bottom ends of the connecting rods 29. The bottom end of the mounting plate 25 is provided with a plurality of temperature detection probes 1;

A driven bevel gear 27 is fixed at the bottom end of the rotating shaft 28, a plurality of telescopic rods 23 are installed at the top ends of the mounting plates 25, one end of each telescopic rod 23 is connected with the top end inside the top cover 12, two groups of fixing blocks 4 are fixed at the top end inside the top cover 12, two fixing blocks 4 are arranged in each group, rotating rods 3 are installed inside the two groups of fixing blocks 4 through bearings, One end of each rotating rod 3 extends to the outer side of the top cover 12 and is provided with a knob, and the other end of each rotating rod 3 extends to the upper part of the mounting plate 7

Specification

LU502877 and is provided with a driving bevel gear 24;

The driving bevel gear 24 and the driven bevel gear 27 have the same size, and the driving bevel gear 24 and the driven bevel gear 27 are engaged with each other;

The outer wall of the rotating shaft 28 is provided with an external thread, and the inner wall of the movable plate 26 is provided with an internal thread matched with the external thread;

The bottom end of the mounting plate 25 is bonded with a heat insulation pad 11, and one end of the temperature detection probe 1 penetrates below the heat insulation pad 11;

Specifically, as shown in fig. 1 and fig. 4, by rotating the rotating rod 3, the rotating shaft 28 is rotated by the cooperation between the driving bevel gear 24 and the driven bevel gear 27, so that the movable plate 26 is moved down, and the mounting plate 25 is pushed down through the connecting rod 29, so that the heat insulating pad 11 is pressed against the chip board 9 and the temperature detecting probe 1 is inserted into the inside of the top cover 12.

The working principle is that when the device is used, the temperature measurement operation is started after the preparation work of the chip is finished, the top cover 12 is buckled, then the two shifting blocks 18 are pulled inward to make the moving bars 17 close to pull the clamping block 16 into the inserting block 13, and then the inserting block 13 is inserted into the clamping box 7 and the shifting block 18 is loosened. The telescopic spring rod 15 pushes the moving bar 17 outward to insert the clamping block 16 into the clamping groove 14, so that the connection between the mounting box 2 and the clamping box 7 is completed, 8

Specification

LU502877 and the top cover 12 is quickly closed.

At this time, the temperature detection probe 1 is positioned above the top cover 12, and then the rotating rod 3 is rotated to rotate the rotating shaft 28 by the cooperation between the driving bevel gear 24 and the driven bevel gear 27, so that the movable plate 26 is moved down, and the mounting plate 25 is pushed down through the connecting rod 29 to press the heat insulation pad 11 on the chip board 9. The temperature detection probe 1 is inserted into the top cover 12 to measure the temperature of the chip at multiple points, and the control module 20 records the measured temperature and displays it on the display module 22.

It will be apparent to those skilled in the art that the present invention is not limited to the details of the exemplary embodiments described above and that the present invention can be embodied in other specific forms without departing from the spirit or essential characteristics thereof. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being defined by the appended claims, rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference signs in the claims shall not be construed as limiting the claim to which they relate. 9

Claims

1. A digital PCR microfluidic chip temperature measurement module, LU502877 comprising a placing box (10), wherein a chip board (9) is arranged inside the placing box (10), a plurality of groups of grooves (8) are formed in the top end of the chip board (9), and sample adding holes (6) are mounted at both ends of the top end of the chip board (8). One end of the placing box (10) is provided with a top cover (12) through a hinged part, and the inside and the top end of the top cover (12) are provided with a detection assembly. The device is characterized in that a plurality of L-shaped limiting plates (5) are arranged on the outer side of the bottom end inside the placing box (10), and two rotating shafts (28) are arranged at the top end inside the top cover (2) through rotating shafts. And the outer wall of the rotating shaft (28) is sleeved with a movable plate (26); the bottom end of the movable plate (26) is provided with a plurality of connecting rods (29); the bottom end of each connecting rod (29) is fixedly provided with a mounting plate (25); and the bottom ends of the mounting plates (25) are provided with a plurality of temperature detection probes (1).

2. The digital PCR microfluidic chip temperature measurement module according to claim 1, wherein the detection assembly comprises a display module (22), the display module (22) is mounted on the top end of the top cover (12), and the top end of the top cover (12) is internally provided with a cavity (19); And a power module (21) is mounted at the other end of the bottom inside the cavity (19).

3. The digital PCR microfluidic chip temperature measurement module according to claim 1, wherein a driven bevel gear (27) is fixed at the bottom end of the rotating shaft (28), a plurality of telescopic rods (23) are mounted at the top end of the mounting plate (25), and one end of each telescopic rod (23) is connected to the top end inside the top cover (12). Two groups of fixed blocks (4) are fixed at

Claims the top end of the inside of the top cover (12), two fixed blocks (4) are arranged in | LU502877 each group, rotating rods (3) are arranged in the two groups of fixed blocks through bearings, a knob is arranged at one end of each rotating rod (3) extending to the outer side of the top cover (12), And the other end of the rotating rod (3) extends to the upper part of the mounting plate (25) and is provided with a driving bevel gear (24).

4. The digital PCR microfluidic chip temperature measurement module according to claim 3, wherein the driving bevel gear (24) and the driven bevel gear (27) have the same size, and the driving bevel gear (24) is engaged with the driven conical gear (27).

5. The digital PCR microfluidic chip temperature measurement module according to claim 1, wherein the bottom end of the mounting plate (25) is bonded with a heat insulating pad (11), and one end of the temperature detection probe (1) penetrates below the heat insulating pad (11).

6. The digital PCR microfluidic chip temperature measurement module according to claim 1, wherein the outer wall of the rotating shaft (28) is provided with an external thread, and the inner wall of the movable plate (26) is provided with an internal thread matched with the external thread.

7. The digital PCR microfluidic chip temperature measurement module according to claim 1, wherein two clamping boxes (7) are fixed at one end of the placing box (10), and clamping grooves (14) are formed on both sides inside the clamping boxes (7), two mounting boxes (2) are fixed at one end of the top cover (12), Inserting blocks (13) are arranged inside the mounting box (2) in a sliding manner, the bottom ends of the inserting blocks (13) extend to the inside of the clamping box (7), moving strips (17) are arranged on two sides inside the inserting 11

Claims blocks in a sliding manner, clamping blocks (16) are fixed at the bottom ends of LU502877 one sides of the moving strips (17), And one end of each clamping block (16) extends to the inside of the clamping groove (14); a plurality of telescopic spring rods (15) are arranged between one sides of the two movable bars (17); a poking block (18) is fixed at one end of each movable bar (17); one end of the poking block (18) extends to an end of the mounting box (2); The bottom ends of the mounting box (2) and the clamping box (7) are positioned on the same horizontal line. 12