LU100716B1 - Assay components for diagnostic in vitro applications - Google Patents
Assay components for diagnostic in vitro applications Download PDFInfo
- Publication number
- LU100716B1 LU100716B1 LU100716A LU100716A LU100716B1 LU 100716 B1 LU100716 B1 LU 100716B1 LU 100716 A LU100716 A LU 100716A LU 100716 A LU100716 A LU 100716A LU 100716 B1 LU100716 B1 LU 100716B1
- Authority
- LU
- Luxembourg
- Prior art keywords
- container
- assay component
- immobilized
- component
- assay
- Prior art date
Links
- 238000003556 assay Methods 0.000 title claims abstract description 35
- 238000000338 in vitro Methods 0.000 title claims abstract description 14
- 238000000034 method Methods 0.000 claims abstract description 13
- 230000003100 immobilizing effect Effects 0.000 claims description 14
- 239000000126 substance Substances 0.000 claims description 12
- 239000002245 particle Substances 0.000 claims description 7
- 239000006249 magnetic particle Substances 0.000 claims description 6
- 150000004676 glycans Chemical class 0.000 claims description 5
- 150000002772 monosaccharides Chemical class 0.000 claims description 5
- 150000007523 nucleic acids Chemical class 0.000 claims description 5
- 102000039446 nucleic acids Human genes 0.000 claims description 5
- 108020004707 nucleic acids Proteins 0.000 claims description 5
- 150000002482 oligosaccharides Chemical class 0.000 claims description 5
- 229920001282 polysaccharide Polymers 0.000 claims description 5
- 239000005017 polysaccharide Substances 0.000 claims description 5
- 102000004196 processed proteins & peptides Human genes 0.000 claims description 5
- 108090000765 processed proteins & peptides Proteins 0.000 claims description 5
- 108010010803 Gelatin Proteins 0.000 claims description 4
- 241000700605 Viruses Species 0.000 claims description 4
- 239000000499 gel Substances 0.000 claims description 4
- 229920000159 gelatin Polymers 0.000 claims description 4
- 239000008273 gelatin Substances 0.000 claims description 4
- 235000019322 gelatine Nutrition 0.000 claims description 4
- 235000011852 gelatine desserts Nutrition 0.000 claims description 4
- 244000005700 microbiome Species 0.000 claims description 4
- 229920001542 oligosaccharide Polymers 0.000 claims description 4
- 235000010603 pastilles Nutrition 0.000 claims description 4
- 229920001817 Agar Polymers 0.000 claims description 3
- 229920000936 Agarose Polymers 0.000 claims description 3
- 239000008272 agar Substances 0.000 claims description 3
- 150000001413 amino acids Chemical class 0.000 claims description 3
- 238000001035 drying Methods 0.000 claims description 3
- 239000007938 effervescent tablet Substances 0.000 claims description 3
- 238000004108 freeze drying Methods 0.000 claims description 3
- 239000002184 metal Substances 0.000 claims description 3
- 229910052751 metal Inorganic materials 0.000 claims description 3
- 235000011837 pasties Nutrition 0.000 claims description 3
- 108090000623 proteins and genes Proteins 0.000 claims description 3
- 102000004169 proteins and genes Human genes 0.000 claims description 3
- 230000000087 stabilizing effect Effects 0.000 claims description 3
- 102000004190 Enzymes Human genes 0.000 claims description 2
- 108090000790 Enzymes Proteins 0.000 claims description 2
- 239000007864 aqueous solution Substances 0.000 claims description 2
- 150000001720 carbohydrates Chemical class 0.000 claims description 2
- 235000014633 carbohydrates Nutrition 0.000 claims description 2
- 239000003814 drug Substances 0.000 claims description 2
- 229940079593 drug Drugs 0.000 claims description 2
- 239000011521 glass Substances 0.000 claims description 2
- 239000003446 ligand Substances 0.000 claims description 2
- 150000002632 lipids Chemical class 0.000 claims description 2
- 239000002923 metal particle Substances 0.000 claims description 2
- 239000000693 micelle Substances 0.000 claims description 2
- 239000002105 nanoparticle Substances 0.000 claims description 2
- 239000004033 plastic Substances 0.000 claims description 2
- 229920000642 polymer Polymers 0.000 claims description 2
- 239000007790 solid phase Substances 0.000 claims description 2
- 239000003826 tablet Substances 0.000 claims description 2
- 239000003349 gelling agent Substances 0.000 claims 3
- 239000011248 coating agent Substances 0.000 claims 1
- 238000000576 coating method Methods 0.000 claims 1
- 150000004696 coordination complex Chemical class 0.000 claims 1
- 238000004090 dissolution Methods 0.000 claims 1
- 239000007937 lozenge Substances 0.000 claims 1
- 239000000463 material Substances 0.000 claims 1
- 238000006243 chemical reaction Methods 0.000 description 37
- 239000011324 bead Substances 0.000 description 35
- 239000000376 reactant Substances 0.000 description 30
- 239000007788 liquid Substances 0.000 description 6
- 230000005291 magnetic effect Effects 0.000 description 5
- 239000000725 suspension Substances 0.000 description 5
- 239000003795 chemical substances by application Substances 0.000 description 4
- 238000005259 measurement Methods 0.000 description 4
- 238000003860 storage Methods 0.000 description 4
- 238000009739 binding Methods 0.000 description 3
- 239000000872 buffer Substances 0.000 description 3
- 238000009826 distribution Methods 0.000 description 3
- 239000013610 patient sample Substances 0.000 description 3
- 238000003756 stirring Methods 0.000 description 3
- 238000002965 ELISA Methods 0.000 description 2
- 239000012062 aqueous buffer Substances 0.000 description 2
- 238000001704 evaporation Methods 0.000 description 2
- 230000008020 evaporation Effects 0.000 description 2
- 238000001879 gelation Methods 0.000 description 2
- 239000006193 liquid solution Substances 0.000 description 2
- 238000002156 mixing Methods 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- HDTRYLNUVZCQOY-UHFFFAOYSA-N α-D-glucopyranosyl-α-D-glucopyranoside Natural products OC1C(O)C(O)C(CO)OC1OC1C(O)C(O)C(O)C(CO)O1 HDTRYLNUVZCQOY-UHFFFAOYSA-N 0.000 description 1
- 229910000831 Steel Inorganic materials 0.000 description 1
- HDTRYLNUVZCQOY-WSWWMNSNSA-N Trehalose Natural products O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-WSWWMNSNSA-N 0.000 description 1
- 239000000853 adhesive Substances 0.000 description 1
- 230000001070 adhesive effect Effects 0.000 description 1
- 238000001042 affinity chromatography Methods 0.000 description 1
- 238000013019 agitation Methods 0.000 description 1
- HDTRYLNUVZCQOY-LIZSDCNHSA-N alpha,alpha-trehalose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-LIZSDCNHSA-N 0.000 description 1
- 239000012491 analyte Substances 0.000 description 1
- -1 antibodies Chemical class 0.000 description 1
- 238000003491 array Methods 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 239000012504 chromatography matrix Substances 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 230000001627 detrimental effect Effects 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 239000000834 fixative Substances 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 239000006260 foam Substances 0.000 description 1
- 230000005714 functional activity Effects 0.000 description 1
- 125000000524 functional group Chemical group 0.000 description 1
- 239000013029 homogenous suspension Substances 0.000 description 1
- 238000011068 loading method Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 150000002739 metals Chemical class 0.000 description 1
- 238000002493 microarray Methods 0.000 description 1
- 238000004806 packaging method and process Methods 0.000 description 1
- 230000005298 paramagnetic effect Effects 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 239000011535 reaction buffer Substances 0.000 description 1
- 239000011541 reaction mixture Substances 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000010959 steel Substances 0.000 description 1
- 230000002311 subsequent effect Effects 0.000 description 1
- 235000000346 sugar Nutrition 0.000 description 1
- 150000008163 sugars Chemical class 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
- G01N33/54326—Magnetic particles
- G01N33/5434—Magnetic particles using magnetic particle immunoreagent carriers which constitute new materials per se
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
- G01N33/54346—Nanoparticles
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54393—Improving reaction conditions or stability, e.g. by coating or irradiation of surface, by reduction of non-specific binding, by promotion of specific binding
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2446/00—Magnetic particle immunoreagent carriers
Landscapes
- Health & Medical Sciences (AREA)
- Immunology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Analytical Chemistry (AREA)
- Biotechnology (AREA)
- Pathology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Cell Biology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Nanotechnology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Claims (16)
1. Ein Behâlter, umfassend mindestens eine Assaykomponente fur eine in-vitro-diagnostische Anwendung, die an einer inneren Oberflâche des Behälters immobilisiert ist und anhaftet.
2. Der Behälter nach Anspruch 1, dadurch gekennzeichnet, dass die Assaykomponente an der inneren Oberflâche des Behältnisses in pastenartiger Form, als Gelpastille, als Tablette oder als lyophilisierte Beschichtungsapplikation angebracht ist.
3. Der Behälter nach einem der Ansprüche 1 oder 2, wobei die immobilisierte Assay-Komponente mindestens eine Komponente ist, ausgewählt aus der Grappe umfassend Arzneimittel, chemische oder biochemische Substanzen, Aminosäuren, Peptide, Proteine, Nukleinsäuren, Kohlenhydrate, Antikörper umfasst Lipide, Micellen, Vesikel, synthetische Moleküle, Polymère, Metallpartikel, Nanopartikel, Festphasen-Zellen, Mikroorganismen, Viren und magnetische Partikel.
4. Der Behâlter nach Anspruch 4, wobei die magnetischen Partikel mindestens eine funktionelle Grappe umfassen, ausgewählt aus der Grappe umfassend Peptide, Nukleinsäuren, monoklonale oder polyklonale Antikörper.
5. Der Behâlter nach einem der Ansprüche 3 bis 6, wobei die magnetischen Partikel nicht-kovalent an einen Liganden gebunden sind, der mit mindestens einem von einem Rezeptor, einem Enzym, einem Metallkomplex oder einem chemischen oder biochemischen Komplex interagiert.
6. Der Behâlter nach einem der Ansprüche 1 bis 5, wobei die immobilisierte Assaykomponente in einer wâssrigen Lösung löslich ist.
7. Der Behâlter nach einem der Ansprüche 1 bis 6, wobei die immobilisierte Assaykomponente mit mindestens einer immobilisierenden Komponente immobilisiert ist, ausgewählt aus der Grappe umfassend Monosaccharide, Oligosaccharide, Polysaccharide und Geliermittel.
8. Der Behälter nach Anspruch 7, wobei das Geliermittel ausgewählt ist aus der Gruppe umfassend Agarose, Agar und Gelatine.
9. Der Behälter nach einem der Ansprüche 1 bis 8, wobei der Behälter Teil einer Kartusche ist, die mindestens eine weitere Assaykomponente umfasst oder ein Röhrchen, eine Flasche, eine Multi-Well-Platte, eine Ampulle mit Deckel oder eine Küvette ist.
10. Der Behälter nach einem der Ansprüche 1 bis 9, wobei der Behälter aus einem Material besteht, ausgewählt aus der Gruppe umfassend Kunststoff, Glas oder Metall.
11. Eine Verwendung eines Behälters nach einem der Ansprüche 1 bis 10 zur Durchführung eines Assays.
12. Ein Verfahren zum Immobilisieren einer Assaykomponente, umfassend den Schritt des Immobilisierens der Assaykomponente mit mindestens einer stabilisierenden Komponente, ausgewählt aus der Gruppe umfassend Monosaccharide, Oligosaccharide, Polysaccharide und Geliermittel.
13. Das Verfahren nach Anspruch 12, wobei die Assaykomponente bio-reaktive Partikel umfasst.
14. Das Verfahren nach Anspruch 12 oder 13, wobei der Immobilisierungsschritt femer Oberflächentrocknen, Lyophilisieren, Gelieren oder Auflösen in hochviskosem Medium beinhaltet.
15. Das Verfahren nach einem der Ansprüche 12 bis 14, wobei der Immobilisierungsschritt femer den Schritt des Aufbringens der Assaykomponente in pastöser oder lyophilisierter Form auf die Behälterwand oder -boden umfasst.
16. Das Verfahren nach einem der Ansprüche 12 bis 15, wobei der Immobilisierungsschritt weiterhin den Schritt des Einbringens der immobilisierten Assaykomponente in das Behältnis in Form einer Brausetablette oder Gelpastille umfasst.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| LU100716A LU100716B1 (en) | 2018-02-26 | 2018-02-26 | Assay components for diagnostic in vitro applications |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| LU100716A LU100716B1 (en) | 2018-02-26 | 2018-02-26 | Assay components for diagnostic in vitro applications |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| LU100716B1 true LU100716B1 (en) | 2019-08-28 |
Family
ID=62067762
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| LU100716A LU100716B1 (en) | 2018-02-26 | 2018-02-26 | Assay components for diagnostic in vitro applications |
Country Status (1)
| Country | Link |
|---|---|
| LU (1) | LU100716B1 (de) |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2002090983A2 (de) * | 2001-05-10 | 2002-11-14 | Medsystems Diagnostics Gmbh | Quantitaver einschritt immuntest in lyophilisierter form |
| US20030108973A1 (en) * | 1999-11-03 | 2003-06-12 | Science And Technology Corp. | Immunoassay and reagents and kits for performing the same |
| US20050048667A1 (en) * | 2003-08-29 | 2005-03-03 | Brett Ellman | Method of forming and using solid-phase support |
| US20050164408A1 (en) * | 2003-01-28 | 2005-07-28 | Boss Gerry R. | Solid phase isolation of proteins, nucleic acids and other macromolecules |
| WO2006137787A1 (en) * | 2005-06-21 | 2006-12-28 | Ge Healthcare Bio-Sciences Ab | Method for cell culture |
| US20150218613A1 (en) * | 2013-10-29 | 2015-08-06 | GeneWeave Biosciences, Inc. | Reagent cartridge and methods for detection of cells |
-
2018
- 2018-02-26 LU LU100716A patent/LU100716B1/en active IP Right Grant
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20030108973A1 (en) * | 1999-11-03 | 2003-06-12 | Science And Technology Corp. | Immunoassay and reagents and kits for performing the same |
| WO2002090983A2 (de) * | 2001-05-10 | 2002-11-14 | Medsystems Diagnostics Gmbh | Quantitaver einschritt immuntest in lyophilisierter form |
| US20050164408A1 (en) * | 2003-01-28 | 2005-07-28 | Boss Gerry R. | Solid phase isolation of proteins, nucleic acids and other macromolecules |
| US20050048667A1 (en) * | 2003-08-29 | 2005-03-03 | Brett Ellman | Method of forming and using solid-phase support |
| WO2006137787A1 (en) * | 2005-06-21 | 2006-12-28 | Ge Healthcare Bio-Sciences Ab | Method for cell culture |
| US20150218613A1 (en) * | 2013-10-29 | 2015-08-06 | GeneWeave Biosciences, Inc. | Reagent cartridge and methods for detection of cells |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CA2886777C (en) | Preloaded test substrates for testing lal-reactive substances, methods of use, and methods of making | |
| AU2008235291B2 (en) | Blood group antibody screening | |
| US20020001839A1 (en) | Apparatus and method for conducting chemical or biochemical reactions on a solid surface within an enclosed chamber | |
| US20090069200A1 (en) | System for Preparing Arrays of Biomolecules | |
| US8916108B2 (en) | Device for distributing particles in a fluid and methods thereof | |
| JP7361492B2 (ja) | 試験試料に含有される干渉物質を分離するための検査室システムおよび方法 | |
| US20170176303A1 (en) | Method and device for transferring liquids | |
| EP2742362A1 (de) | Verfahren und vorrichtung zur automatisierten analyse | |
| LU100716B1 (en) | Assay components for diagnostic in vitro applications | |
| JPH11160314A (ja) | 分析的測定法及びその使用 | |
| HUP0102588A2 (hu) | Eszköz, eljárás és készülék legalább egy összetevőnek termékmintába való beadagolására | |
| US20240269670A1 (en) | Methods, Systems, and Devices for Linear Electrowetting Cartridges | |
| Taitt et al. | Antimicrobial peptide arrays for detection of inactivated biothreat agents | |
| US20210311084A1 (en) | Sample processing | |
| US20250121374A1 (en) | Solution replacement method and digital assay method | |
| AU2013204763B2 (en) | Device for distributing particles in a fluid and methods therefor | |
| US20100035285A1 (en) | Rapid elisa | |
| US20060223130A1 (en) | Detection of post-translationally modified analytes | |
| Stein | Membrane chromatography-high throughput screening and simulative process develoopment | |
| WO2025014995A1 (en) | Drug screening systems, apparatuses, and methods | |
| EP1566217A2 (de) | Kammerreihe | |
| Park et al. | Chemical microarrays constructed by selective attachment of hydrazide-conjugated substances to epoxide surfaces and their applications | |
| Viitala | New applications for immunoassay based protein microarrays | |
| HK1223152B (zh) | 用於固相和液相之间的反应的改进的设备和方法 |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FG | Patent granted |
Effective date: 20190828 |