LTIP595A - Method of preparing ribonuclease dimers - Google Patents
Method of preparing ribonuclease dimersInfo
- Publication number
- LTIP595A LTIP595A LTIP595A LTIP595A LTIP595A LT IP595 A LTIP595 A LT IP595A LT IP595 A LTIP595 A LT IP595A LT IP595 A LTIP595 A LT IP595A LT IP595 A LTIP595 A LT IP595A
- Authority
- LT
- Lithuania
- Prior art keywords
- ribonuclease
- dimers
- preparing
- dimer
- product
- Prior art date
Links
- 108010083644 Ribonucleases Proteins 0.000 title abstract 5
- 102000006382 Ribonucleases Human genes 0.000 title abstract 5
- 239000000539 dimer Substances 0.000 title abstract 4
- 238000000034 method Methods 0.000 title abstract 3
- 239000000178 monomer Substances 0.000 abstract 2
- NWUYHJFMYQTDRP-UHFFFAOYSA-N 1,2-bis(ethenyl)benzene;1-ethenyl-2-ethylbenzene;styrene Chemical class C=CC1=CC=CC=C1.CCC1=CC=CC=C1C=C.C=CC1=CC=CC=C1C=C NWUYHJFMYQTDRP-UHFFFAOYSA-N 0.000 abstract 1
- 208000035143 Bacterial infection Diseases 0.000 abstract 1
- 208000036142 Viral infection Diseases 0.000 abstract 1
- 208000022362 bacterial infectious disease Diseases 0.000 abstract 1
- 230000009286 beneficial effect Effects 0.000 abstract 1
- 238000006243 chemical reaction Methods 0.000 abstract 1
- 239000003153 chemical reaction reagent Substances 0.000 abstract 1
- 238000011210 chromatographic step Methods 0.000 abstract 1
- 230000008878 coupling Effects 0.000 abstract 1
- 238000010168 coupling process Methods 0.000 abstract 1
- 238000005859 coupling reaction Methods 0.000 abstract 1
- 231100000433 cytotoxic Toxicity 0.000 abstract 1
- 230000001472 cytotoxic effect Effects 0.000 abstract 1
- 239000007862 dimeric product Substances 0.000 abstract 1
- 230000000447 dimerizing effect Effects 0.000 abstract 1
- 238000001914 filtration Methods 0.000 abstract 1
- 238000001502 gel electrophoresis Methods 0.000 abstract 1
- 239000003456 ion exchange resin Substances 0.000 abstract 1
- 229920003303 ion-exchange polymer Polymers 0.000 abstract 1
- 238000012544 monitoring process Methods 0.000 abstract 1
- 239000000047 product Substances 0.000 abstract 1
- 108010015937 ribonuclease dimer Proteins 0.000 abstract 1
- 230000003612 virological effect Effects 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/04—Antibacterial agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/12—Antivirals
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Medicinal Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Pharmacology & Pharmacy (AREA)
- Oncology (AREA)
- Veterinary Medicine (AREA)
- General Engineering & Computer Science (AREA)
- Animal Behavior & Ethology (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- General Chemical & Material Sciences (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Microbiology (AREA)
- Public Health (AREA)
- Communicable Diseases (AREA)
- Biochemistry (AREA)
- Virology (AREA)
- Enzymes And Modification Thereof (AREA)
- Compounds Of Unknown Constitution (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Exposure Control For Cameras (AREA)
- Structure And Mechanism Of Cameras (AREA)
- Adjustment Of Camera Lenses (AREA)
- Studio Devices (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
A method for preparing purified ribonuclease dimers is provided which comprise the steps of dimerizing monomeric ribonuclease with a suberimidate coupling reagent, filtering out undimerized monomers and oligomers from the dimer solution using a series of ion exchange resin chromatography steps, monitoring the progress of the reaction through gel electrophoresis, and collecting the purified ribonuclease dimer product. The method is advantageous in that large amounts of the dimeric product can be obtained efficiently and relatively inexpensively, and the ribonuclease dimers so produced can be employed for their beneficial effects in combatting viral and bacterial infections without the cytotoxic effects normally associated with the ribonuclease monomer.
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PCT/US1990/000141 WO1991010730A1 (en) | 1990-01-08 | 1990-01-08 | Method of preparing ribonuclease dimers |
| HU9202251A HU212929B (en) | 1990-01-08 | 1990-01-08 | Method of preraring ribonuclease dimers |
| SG1996003125A SG47607A1 (en) | 1990-01-08 | 1990-01-08 | Method of preparing ribonuclease dimers |
| SU905052819A RU2067617C1 (en) | 1990-01-08 | 1990-01-08 | Method of lysozyme dimer preparing |
| OA60243A OA09707A (en) | 1990-01-08 | 1992-07-07 | Method of preparing ribonuclease dimers |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| LTIP595A true LTIP595A (en) | 1994-12-27 |
| LT3424B LT3424B (en) | 1995-09-25 |
Family
ID=33136228
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| LTIP595A LT3424B (en) | 1990-01-08 | 1993-06-02 | Method of preparing ribonuclease dimers |
Country Status (21)
| Country | Link |
|---|---|
| EP (1) | EP0509989B1 (en) |
| JP (1) | JP2732515B2 (en) |
| KR (1) | KR0142172B1 (en) |
| AT (1) | ATE136056T1 (en) |
| AU (1) | AU645252B2 (en) |
| BG (1) | BG60685B1 (en) |
| BR (1) | BR9007970A (en) |
| DE (1) | DE69026275T2 (en) |
| DK (1) | DK0509989T3 (en) |
| ES (1) | ES2086403T3 (en) |
| FI (1) | FI100724B (en) |
| HK (1) | HK166796A (en) |
| HU (1) | HU212929B (en) |
| LT (1) | LT3424B (en) |
| LV (1) | LV10118B (en) |
| NO (1) | NO922625D0 (en) |
| OA (1) | OA09707A (en) |
| PL (1) | PL165863B1 (en) |
| RU (2) | RU2067617C1 (en) |
| SG (1) | SG47607A1 (en) |
| WO (1) | WO1991010730A1 (en) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DZ1964A1 (en) * | 1995-01-13 | 2002-10-15 | Nika Health Products Ltd | New applications of a lysozyme dimer. |
| WO2005012911A1 (en) * | 2003-07-29 | 2005-02-10 | Solvay Pharmaceuticals Gmbh | Analytical method for pancreatin and comparable compositions |
| FR3115037B1 (en) * | 2020-10-12 | 2025-02-21 | Cie Laitiere Europeenne | Process for purifying cationic protein fraction and fraction thus obtained |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5200182A (en) * | 1988-05-26 | 1993-04-06 | Nika Health Products, Ltd. | Antiviral or antibacterial composition and method of use |
| US7850731B2 (en) | 2006-10-04 | 2010-12-14 | Seaspine, Inc. | Articulating spinal implant |
-
1990
- 1990-01-08 RU SU905052819A patent/RU2067617C1/en not_active IP Right Cessation
- 1990-01-08 ES ES90907615T patent/ES2086403T3/en not_active Expired - Lifetime
- 1990-01-08 DE DE69026275T patent/DE69026275T2/en not_active Expired - Fee Related
- 1990-01-08 AT AT90907615T patent/ATE136056T1/en not_active IP Right Cessation
- 1990-01-08 SG SG1996003125A patent/SG47607A1/en unknown
- 1990-01-08 EP EP90907615A patent/EP0509989B1/en not_active Expired - Lifetime
- 1990-01-08 KR KR1019920701567A patent/KR0142172B1/en not_active Expired - Fee Related
- 1990-01-08 BR BR909007970A patent/BR9007970A/en not_active Application Discontinuation
- 1990-01-08 AU AU56401/90A patent/AU645252B2/en not_active Ceased
- 1990-01-08 RU SU905052835A patent/RU2060274C1/en not_active IP Right Cessation
- 1990-01-08 JP JP2507450A patent/JP2732515B2/en not_active Expired - Fee Related
- 1990-01-08 DK DK90907615.0T patent/DK0509989T3/en active
- 1990-01-08 WO PCT/US1990/000141 patent/WO1991010730A1/en not_active Ceased
- 1990-01-08 HU HU9202251A patent/HU212929B/en unknown
- 1990-12-14 PL PL90288268A patent/PL165863B1/en not_active IP Right Cessation
-
1992
- 1992-07-03 NO NO1992922625A patent/NO922625D0/en unknown
- 1992-07-07 FI FI923124A patent/FI100724B/en active
- 1992-07-07 OA OA60243A patent/OA09707A/en unknown
- 1992-07-07 BG BG96579A patent/BG60685B1/en unknown
- 1992-12-28 LV LVP-92-507A patent/LV10118B/en unknown
-
1993
- 1993-06-02 LT LTIP595A patent/LT3424B/en not_active IP Right Cessation
-
1996
- 1996-09-05 HK HK166796A patent/HK166796A/en not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| NO922625D0 (en) | 1992-07-03 |
| AU5640190A (en) | 1991-08-05 |
| WO1991010730A1 (en) | 1991-07-25 |
| HK166796A (en) | 1996-09-13 |
| HU212929B (en) | 1996-12-30 |
| BG60685B1 (en) | 1995-12-29 |
| OA09707A (en) | 1993-08-30 |
| KR0142172B1 (en) | 1998-07-01 |
| EP0509989B1 (en) | 1996-03-27 |
| BG96579A (en) | 1993-12-24 |
| DE69026275T2 (en) | 1996-10-02 |
| FI923124L (en) | 1992-07-07 |
| PL165863B1 (en) | 1995-02-28 |
| ATE136056T1 (en) | 1996-04-15 |
| FI923124A0 (en) | 1992-07-07 |
| HUT65395A (en) | 1994-06-28 |
| RU2067617C1 (en) | 1996-10-10 |
| LV10118B (en) | 1995-02-20 |
| DE69026275D1 (en) | 1996-05-02 |
| EP0509989A1 (en) | 1992-10-28 |
| FI100724B (en) | 1998-02-13 |
| JPH05505094A (en) | 1993-08-05 |
| AU645252B2 (en) | 1994-01-13 |
| BR9007970A (en) | 1992-11-17 |
| JP2732515B2 (en) | 1998-03-30 |
| DK0509989T3 (en) | 1996-07-08 |
| PL288268A1 (en) | 1991-09-09 |
| ES2086403T3 (en) | 1996-07-01 |
| LT3424B (en) | 1995-09-25 |
| RU2060274C1 (en) | 1996-05-20 |
| KR920703802A (en) | 1992-12-18 |
| SG47607A1 (en) | 1998-04-17 |
| LV10118A (en) | 1994-05-10 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| MM9A | Lapsed patents |
Effective date: 20020602 |