LTIP276A - Method for determination of glucose in whole blood, single use cuvette and photometer for carrying out said method - Google Patents

Method for determination of glucose in whole blood, single use cuvette and photometer for carrying out said method

Info

Publication number
LTIP276A
LTIP276A LTIP276A LTIP276A LTIP276A LT IP276 A LTIP276 A LT IP276A LT IP276 A LTIP276 A LT IP276A LT IP276 A LTIP276 A LT IP276A LT IP276 A LTIP276 A LT IP276A
Authority
LT
Lithuania
Prior art keywords
sample
glucose
pct
reagent
cavity
Prior art date
Application number
LTIP276A
Inventor
Jan Evert Lilja
Sven-Erik Lennart Nilsson
Original Assignee
Migrata Uk Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Migrata Uk Ltd filed Critical Migrata Uk Ltd
Publication of LTIP276A publication Critical patent/LTIP276A/en
Publication of LT3187B publication Critical patent/LT3187B/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/54—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving glucose or galactose
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/01—Arrangements or apparatus for facilitating the optical investigation
    • G01N21/03—Cuvette constructions
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
    • G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
    • G01N21/314—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry with comparison of measurements at specific and non-specific wavelengths
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/01—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials specially adapted for biological cells, e.g. blood cells
    • G01N2015/011—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials specially adapted for biological cells, e.g. blood cells with lysing, e.g. of erythrocytes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/01—Arrangements or apparatus for facilitating the optical investigation
    • G01N21/03—Cuvette constructions
    • G01N2021/0321—One time use cells, e.g. integrally moulded
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/01—Arrangements or apparatus for facilitating the optical investigation
    • G01N21/03—Cuvette constructions
    • G01N2021/0325—Cells for testing reactions, e.g. containing reagents
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/01—Arrangements or apparatus for facilitating the optical investigation
    • G01N21/03—Cuvette constructions
    • G01N2021/0346—Capillary cells; Microcells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
    • G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
    • G01N21/314—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry with comparison of measurements at specific and non-specific wavelengths
    • G01N2021/3181—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry with comparison of measurements at specific and non-specific wavelengths using LEDs
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
    • G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
    • G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2201/00—Features of devices classified in G01N21/00
    • G01N2201/12—Circuits of general importance; Signal processing
    • G01N2201/122—Kinetic analysis; determining reaction rate
    • G01N2201/1222—Endpoint determination; reaction time determination
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2201/00—Features of devices classified in G01N21/00
    • G01N2201/12—Circuits of general importance; Signal processing
    • G01N2201/126—Microprocessor processing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material
    • G01N33/493—Physical analysis of biological material of liquid biological material urine

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Organic Chemistry (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Emergency Medicine (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Optical Measuring Cells (AREA)
  • Measurement Of The Respiration, Hearing Ability, Form, And Blood Characteristics Of Living Organisms (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)

Abstract

PCT No. PCT/SE90/00273 Sec. 371 Date Oct. 16, 1991 Sec. 102(e) Date Oct. 16, 1991 PCT Filed Apr. 24, 1990 PCT Pub. No. WO90/12890 PCT Pub. Date Nov. 1, 1990A sample of whole blood is contacted with a reagent which by chemical reaction with glucose in the sample brings about a detectable dye concentration change (10) the size of which is determined as a measure of the glucose content of the sample. The sample is initially introduced undiluted in a microcuvette having at least one cavity for receiving the sample. The cavity is internally pretreated with the reagent in dry form, and the chemical reaction takes place in the cavity. Active components of the reagent comprise at least a hemolyzing agent for exposing glucose contained in the blood cells of the sample for allowing total glucose determination, and agents taking part in the chemical reaction and ensuring that the dye concentration change (10) takes place at least in a wavelength range (14) outside the absorption range (12) of the blood hemoglobin. An absorption measurement is performed in said wavelength range directly on the sample in the cuvette. A pretreated disposable cuvette with such a reagent and a photometer are also described.
LTIP276A 1989-04-25 1992-12-30 Method for determination of glucose in whole blood, single use cuvette and photometer for carrying out said method LT3187B (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
SE8901514A SE466157B (en) 1989-04-25 1989-04-25 DETERMINED TO DETERMINE THE GLUCOSE CONTENT OF WHOLE BLOOD AND DISPOSABLE BEFORE THIS

Publications (2)

Publication Number Publication Date
LTIP276A true LTIP276A (en) 1994-10-25
LT3187B LT3187B (en) 1995-03-27

Family

ID=20375801

Family Applications (1)

Application Number Title Priority Date Filing Date
LTIP276A LT3187B (en) 1989-04-25 1992-12-30 Method for determination of glucose in whole blood, single use cuvette and photometer for carrying out said method

Country Status (18)

Country Link
US (1) US5866349A (en)
EP (1) EP0469097B1 (en)
JP (1) JPH0734758B2 (en)
KR (1) KR960004040B1 (en)
AT (1) ATE133453T1 (en)
AU (1) AU632569B2 (en)
BR (1) BR9007340A (en)
CA (1) CA2053284C (en)
DE (1) DE69025058T2 (en)
DK (1) DK0469097T3 (en)
ES (1) ES2081987T3 (en)
FI (1) FI102192B1 (en)
LT (1) LT3187B (en)
LV (1) LV10121B (en)
NO (1) NO300854B1 (en)
RU (1) RU2050545C1 (en)
SE (1) SE466157B (en)
WO (1) WO1990012890A1 (en)

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Also Published As

Publication number Publication date
FI102192B (en) 1998-10-30
FI915021A0 (en) 1991-10-24
KR920701475A (en) 1992-08-11
ATE133453T1 (en) 1996-02-15
NO300854B1 (en) 1997-08-04
NO914182D0 (en) 1991-10-24
NO914182L (en) 1991-10-24
EP0469097A1 (en) 1992-02-05
KR960004040B1 (en) 1996-03-25
DE69025058T2 (en) 1996-05-30
DK0469097T3 (en) 1996-02-19
JPH04504662A (en) 1992-08-20
CA2053284C (en) 2001-12-11
LV10121B (en) 1995-02-20
CA2053284A1 (en) 1990-10-26
JPH0734758B2 (en) 1995-04-19
SE8901514L (en) 1990-10-26
WO1990012890A1 (en) 1990-11-01
LV10121A (en) 1994-05-10
BR9007340A (en) 1992-04-21
DE69025058D1 (en) 1996-03-07
ES2081987T3 (en) 1996-03-16
SE466157B (en) 1992-01-07
AU632569B2 (en) 1993-01-07
SE8901514D0 (en) 1989-04-25
FI102192B1 (en) 1998-10-30
EP0469097B1 (en) 1996-01-24
RU2050545C1 (en) 1995-12-20
AU5557690A (en) 1990-11-16
LT3187B (en) 1995-03-27
US5866349A (en) 1999-02-02

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