LT2004108A - Nikuojančių fermentų sukūrimo būdas - Google Patents

Nikuojančių fermentų sukūrimo būdas

Info

Publication number
LT2004108A
LT2004108A LT2004108A LT2004108A LT2004108A LT 2004108 A LT2004108 A LT 2004108A LT 2004108 A LT2004108 A LT 2004108A LT 2004108 A LT2004108 A LT 2004108A LT 2004108 A LT2004108 A LT 2004108A
Authority
LT
Lithuania
Prior art keywords
engineering
nicking endonucleases
bsmai
nicking enzymes
identifying
Prior art date
Application number
LT2004108A
Other languages
English (en)
Other versions
LT5262B (lt
Inventor
Zhenyu Zhu
Shuang Yong Xu
Original Assignee
New England Biolabs, Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by New England Biolabs, Inc filed Critical New England Biolabs, Inc
Publication of LT2004108A publication Critical patent/LT2004108A/lt
Publication of LT5262B publication Critical patent/LT5262B/lt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Pateikiami būdai, skirti naujų grandinei specifinių nikuojančių endonukleazių identifikavimui, panaudojant mutagenizuotų restrikcijos endonukleazės genų in vitro atgalinius kryžminimus su jų laukinio tipo atitikmeniu ir gautų nikuojančių endonukleazių identifikavimą, nustatant jų skaidymo aktyvumą ir specifiškumą grandinei. Nikuojančių endonukleazių, identifikuotų šiuo būdu, pavyzdžiai yra NtBsaI ir Nb.BsaI, Nt.BsmAI ir Nb.BsmAI, ir Nt.BsmBI. ą
LT2004108A 2003-12-19 2004-12-17 Nikuojančių fermentų sukūrimo būdas LT5262B (lt)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US53106403P 2003-12-19 2003-12-19

Publications (2)

Publication Number Publication Date
LT2004108A true LT2004108A (lt) 2005-06-27
LT5262B LT5262B (lt) 2005-09-26

Family

ID=34633012

Family Applications (1)

Application Number Title Priority Date Filing Date
LT2004108A LT5262B (lt) 2003-12-19 2004-12-17 Nikuojančių fermentų sukūrimo būdas

Country Status (2)

Country Link
US (1) US20050136462A1 (lt)
LT (1) LT5262B (lt)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2018045141A1 (en) 2016-09-02 2018-03-08 New England Biolabs, Inc. Analysis of chromatin using a nicking enzyme
WO2019099081A1 (en) 2017-11-16 2019-05-23 New England Biolabs, Inc. Mapping the location, type and strand of damaged and/or mismatched nucleotides in double-stranded dna
US11021701B2 (en) 2017-11-22 2021-06-01 New England Biolabs, Inc. Method for fragmenting DNA by nick translation
US11492667B2 (en) 2020-06-12 2022-11-08 New England Biolabs, Inc. Compositions and methods for detecting molecular targets on chromosomal DNA
US20240376531A1 (en) 2021-06-09 2024-11-14 New England Biolabs, Inc. Target Initiation and Amplification of Long DNA with Nuclease and Replisome Enzymes
WO2025104680A1 (en) 2023-11-17 2025-05-22 Friedrich Miescher Institute For Biomedical Research Dna accessibility for the assessment of male fertility

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6191267B1 (en) * 2000-06-02 2001-02-20 New England Biolabs, Inc. Cloning and producing the N.BstNBI nicking endonuclease
GB0018120D0 (en) * 2000-07-24 2000-09-13 Fermentas Ab Nuclease
US6660475B2 (en) * 2000-12-15 2003-12-09 New England Biolabs, Inc. Use of site-specific nicking endonucleases to create single-stranded regions and applications thereof
US6395523B1 (en) * 2001-06-01 2002-05-28 New England Biolabs, Inc. Engineering nicking endonucleases from type IIs restriction endonucleases
US20030211506A1 (en) * 2001-06-01 2003-11-13 Huimin Kong N. bstnbi nicking endonuclease and methods for using endonucleases in single-stranded displacement amplification
AU2002365122A1 (en) * 2001-08-23 2003-07-15 New England Biolabs, Inc. A method for engineering strand-specific, sequence-specific, dna-nicking enzymes
EP1501950A4 (en) 2002-04-12 2006-03-15 New England Biolabs Inc METHOD AND COMPOSITIONS FOR DNA MANIPULATION

Also Published As

Publication number Publication date
LT5262B (lt) 2005-09-26
US20050136462A1 (en) 2005-06-23

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Legal Events

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Effective date: 20231217