KR980002267A - CDNA of a Novel Thrombin-like Protease - Google Patents

CDNA of a Novel Thrombin-like Protease Download PDF

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Publication number
KR980002267A
KR980002267A KR1019960023014A KR19960023014A KR980002267A KR 980002267 A KR980002267 A KR 980002267A KR 1019960023014 A KR1019960023014 A KR 1019960023014A KR 19960023014 A KR19960023014 A KR 19960023014A KR 980002267 A KR980002267 A KR 980002267A
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South Korea
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cdna
salmobin
amino acids
protease
thrombin
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KR1019960023014A
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Korean (ko)
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KR0173088B1 (en
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고유석
정광회
박동수
윤영대
김두식
문흥모
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허영섭
재단법인 목암생명공학연구소
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6402—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from non-mammals
    • C12N9/6418—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from non-mammals from snakes

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
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  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
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  • Enzymes And Modification Thereof (AREA)

Abstract

본 발명은 한국산 살모사(Korean salmosa snake : Agkistrodon halys brevicaudus)의 독샘으로부터 분리한 신규한 트롬빈 유사 단백질 분해효소인 살모빈 (Salmobin)의 cDNA 및 그로부터 번역되는 아미노산 서열을 가지는 살모빈에 관한 것이다. 살모빈의 cDNA는 260개의 아미노산을 코딩하는 780개 뉴클레오티드로 구성되어 있으며, 그로부터 번역되는 단백질은 24개 아미노산의 시그날 펩타이드와 236개 아미노산의 성숙 효소로 구성되어 있다. 본 발명의 살모빈 cDNA는 재조합 대장균, 효모, 배큘로바이러스/곤충세포 및 동물세포에서 확립된 발현시스템에 의하여 발현될 수 있으며, 이와 같은 방법으로 다량생산된 살모나제는 혈전증 치료제 및 지혈제의 제조에 유용하게 사용될 수 있을 것이다.The present invention relates to a cDNA of Salmobin, a novel thrombin-like proteolytic enzyme isolated from the venom gland of Korean salmosa snake (Agkistrodon halys brevicaudus), and a salmobin having an amino acid sequence translated therefrom. Salmobin's cDNA consists of 780 nucleotides encoding 260 amino acids, and the protein translated therefrom consists of a signal peptide of 24 amino acids and a mature enzyme of 236 amino acids. Salmonine cDNA of the present invention can be expressed by an expression system established in recombinant E. coli, yeast, baculovirus / insect cells and animal cells. It can be useful for.

Description

신규한 트롬빈 유사 단백질 분해효소의 cDNACDNA of a Novel Thrombin-like Protease

본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음Since this is an open matter, no full text was included.

제1도는 한국산 살모사의 독샘 cDNA 라이브러리 제조과정을 나타내는 모식도이다.Figure 1 is a schematic diagram showing the manufacturing process of the venom gDNA library of the Korean salsa.

제2(a)도는 살모빈(Salmobin) cDNA의 염기서열을 나타낸다, 제2(b)도는 제 3(a)도에 개시된 살모빈 cDNA의 염기서열로부터 번역되는 아미노산 서열을 나타낸다.FIG. 2 (a) shows the base sequence of Salmobin cDNA, and FIG. 2 (b) shows the amino acid sequence translated from the base sequence of Salmobin cDNA disclosed in FIG. 3 (a).

제3도는 살모빈 및 공지된 뱀독 세린계 단백질 가수분해효소들의 아미노산 서열을 비교하여 나타낸 것이다.3 shows a comparison of the amino acid sequences of salmobin and known snake venom serine proteolytic enzymes.

Claims (3)

한국산 살모사인 아그키스트로돈 핼리스 브레비카우두스(Agkistrodon halys brevicaudus)의 독샘 cDNA 라이브러리로부터 분리한 하기와 같은 염기서열을 가지는 트롬빈 분해효소 살모빈(Salmobin)의 cDNA :CDNA of thrombin degrading enzyme Salmobin having the following nucleotide sequence isolated from the poison gland cDNA library of the Korean salmolog Agkistrodon halys brevicaudus: 제1항에 있어서, 하기와 같은 올리고 뉴클레오티드를 사용하여 아그키스트로돈 핼리스 브레비카우두스(Agkistrodon halys brevicaudus)의 독샘 cDNA 라이브러리로부터 클로닝되는 것을 특징으로 하는 살모빈의 cDNA :5'-CGTTCCCTTGTTGTCTTGTTTAACTCCAGCG-3'.According to claim 1, cDNA: 5'-CGTTCCCTTGTTGTCTTGTTTAACTCCAGCG- 3 '. 제1항의 트롬빈 유사 단백질 분해효소 살모빈의 cDNA로부터 번역되는 하기와 같은 아미노산 서열을 가지는 살모빈 :Salmonine having the following amino acid sequence translated from cDNA of thrombin-like protease salmobin of claim 1: ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.※ Note: The disclosure is based on the initial application.
KR1019960023014A 1996-06-21 1996-06-21 Novel cdna of thrombin-like serine protease KR0173088B1 (en)

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KR1019960023014A KR0173088B1 (en) 1996-06-21 1996-06-21 Novel cdna of thrombin-like serine protease

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Application Number Priority Date Filing Date Title
KR1019960023014A KR0173088B1 (en) 1996-06-21 1996-06-21 Novel cdna of thrombin-like serine protease

Publications (2)

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KR980002267A true KR980002267A (en) 1998-03-30
KR0173088B1 KR0173088B1 (en) 1999-02-01

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