KR970006503A - Carlobin gene with antithrombotic action - Google Patents

Carlobin gene with antithrombotic action Download PDF

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Publication number
KR970006503A
KR970006503A KR1019950019862A KR19950019862A KR970006503A KR 970006503 A KR970006503 A KR 970006503A KR 1019950019862 A KR1019950019862 A KR 1019950019862A KR 19950019862 A KR19950019862 A KR 19950019862A KR 970006503 A KR970006503 A KR 970006503A
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South Korea
Prior art keywords
carlobin
gene
venom
found
protein
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KR1019950019862A
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Korean (ko)
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KR0143932B1 (en
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김영식
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김영식
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Priority to KR1019950019862A priority Critical patent/KR0143932B1/en
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Publication of KR0143932B1 publication Critical patent/KR0143932B1/en

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  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

본 발명은 한국산 독사의 한 종류(Agkistrodon caliginosus)인 불독사로부터 트롬빈 유사 효소인 칼로빈의 cDNA 및 그로부터 번역되는 칼로빈에 관한 것이다.The present invention relates to a cDNA of a thrombin-like enzyme carbinin and a carlobine translated therefrom from a bulldog, a kind of Korean viper (Agkistrodon caliginosus).

밝혀진 유전자는 24개의 신호 펩타이드를 포함한 아미노산 배열 I을 갖는 단백질을 암호화 한다. 그의 암호화 사슬은 1598개의 뉴크레오타이드를 함유하며 이중 632개는 정지코돈 TGA 다음에 존재한다. 단백질을 암호화하는 부분은 786개의 염기로 구성되어 있음을 알 수 있었다. 밝혀진 유전자는 불독사 독으로부터 정재된 칼로빈과 N-말단이 일치하였고 N-말단을 제외한 나머지 부분도 기존의 발표된 트롬빈 유사 단백질들(Ancrod, Batroxobin, Flavoxobin)과 아미노산 배열서 유사성이 있지만 그 정도는 70% 미만이었다.The found gene encodes a protein having amino acid sequence I comprising 24 signal peptides. Its coding chain contains 1598 nucleotides, of which 632 follow the stop codon TGA. The protein coding portion was found to consist of 786 bases. The genes identified were consistent with N-terminus and carlobin defined from the bull venom venom. Was less than 70%.

Description

항혈전 작용을 갖는 칼로빈 유전자Carlobin gene with antithrombotic action

본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음Since this is an open matter, no full text was included.

제1도는 칼로빈의 N-말단을 도시한다, 제2도는 칼로빈 cDNA 염기 배열 순서를 도시한다, 제3도는 칼로빈의 아미노산 배열 순서 I를 도시한다.FIG. 1 shows the N-terminus of carlobine, FIG. 2 shows the carbinine cDNA nucleotide sequence, and FIG. 3 shows the amino acid sequence I of carlobine.

Claims (3)

제2도의 염기 서열을 갖는 칼로빈 유전자.Carlobin gene having the nucleotide sequence of FIG. 제1항의 칼로빈 유전자로부터 번역되는 제3도에 개시된 바와 같은 아미노산 배열.An amino acid sequence as disclosed in FIG. 3 translated from the carbinin gene of claim 1. DNA배열 5-GTC/GATTGGAGGTGATGAATG-3의 프로우브를 사용하여 불독사 독샘의 유전자 라이브러리를 스크리닝 함으로써 cDNA를 얻는 방법.A method of obtaining cDNA by screening a gene library of bull venom venom using a probe of DNA array 5-GTC / GATTGGAGGTGATGAATG-3. ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.※ Note: The disclosure is based on the initial application.
KR1019950019862A 1995-07-07 1995-07-07 Carlobin gene with antithrombotic action KR0143932B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
KR1019950019862A KR0143932B1 (en) 1995-07-07 1995-07-07 Carlobin gene with antithrombotic action

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Application Number Priority Date Filing Date Title
KR1019950019862A KR0143932B1 (en) 1995-07-07 1995-07-07 Carlobin gene with antithrombotic action

Publications (2)

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KR970006503A true KR970006503A (en) 1997-02-21
KR0143932B1 KR0143932B1 (en) 1998-07-01

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KR1019950019862A KR0143932B1 (en) 1995-07-07 1995-07-07 Carlobin gene with antithrombotic action

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