KR970062694A - Method of manufacturing immune membrane strip - Google Patents

Method of manufacturing immune membrane strip Download PDF

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Publication number
KR970062694A
KR970062694A KR1019960002485A KR19960002485A KR970062694A KR 970062694 A KR970062694 A KR 970062694A KR 1019960002485 A KR1019960002485 A KR 1019960002485A KR 19960002485 A KR19960002485 A KR 19960002485A KR 970062694 A KR970062694 A KR 970062694A
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South Korea
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biotin
avidin
strept
rti
reaction
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KR1019960002485A
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Korean (ko)
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KR100416490B1 (en
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백세환
백운화
이영호
육순학
이창섭
신인수
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백운화
두산기술원 연구조합
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G01N33/54386—Analytical elements
    • G01N33/54387—Immunochromatographic test strips
    • G01N33/54388—Immunochromatographic test strips based on lateral flow
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/544—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being organic

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  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

본 발명은 비오틴-비반응성 단백질 중합체를 멤브레인 표면에 일정지역 물리흡착 부동화시키고, 비반응성 단백질을 이용하여 잔여표면을 처리한 후, 스트렙토아비딘 또는 아비딘 용액을 스틀립의 하단 끝으로부터 기흡착된 비오틴 단백질 지역으로 전달하여 제1차(스트렙트)아비딘-비오틴 반응시키고, 비오틴-항체 중합체 용액을 다시 순차적으로 전달하여 동일한 지역에서 기부동화된 (스트렙트)아비딘과 제2차(스트렙트)아비딘-비오틴 반응에 의해 항체를 부동화시키고, 세척시킴을 특징으로 하는 면역 멤브레인 스트립의 제조방법에 관한 것이다.The present invention relates to a biotin-nonreactive protein polymer which is physically adsorbed and immobilized in a certain region on the surface of a membrane, which is treated with a nonreactive protein, and then a streptavidin or avidin solution is adsorbed from the lower end of the scintillation- (Strept) avidin-biotin reaction, and the biotin-antibody polymer solution is sequentially transferred again to obtain a second (strept) avidin-biotin (strept) avidin- And immobilizing and washing the antibody by the reaction.

Description

면역 멤브레인 스티립의 제조방법.A method for manufacturing an immune membrane streak.

본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음Since this is a trivial issue, I did not include the contents of the text.

제1도는 세공성 멤브레인 스티립을 통한 측면흐름 방식에 의해 아비딘 또는 스트렙트아비딘-비오틴 반응을 유도하여 그 스트립 표면에 항체를 부동화하는 공정을 나타낸 원리도이다.FIG. 1 is a principle diagram showing a step of inducing avidin or streptavidin-biotin reaction by a side flow method through a pleated membrane streak to immobilize an antibody on the surface of the strip.

Claims (8)

비오틴-비반응성 단백질 종합제를 멤브레인 표면에 일정지역 물리흡착 부동화시키고, 비반응성 단백질을 이용하여 잔여표면을 처리한 후, 스트렙토아비딘 또는 아비딘 용액을 스트립의 하단 끝으로부터 기흡착된 비오틴 단백질 지역으로 전달하여 제1차(스트렙트)아비딘-비오틴 반응시키고, 비오틴-항체 중합제 용액을 디시 순차적으로 전달하여 동일한 지역에서 기부동화된 (스트렙트)아비딘과 제2차(스트렙트)아비딘-비오틴 반응에 의해 항체를 부동화시키고, 세척시킴을 특징으로 하는 면역 멤브레인 스트립의 제조방법.The biotin-non-reactive protein synthesis agent is physically adsorbed and immobilized in a certain region on the surface of the membrane, the remaining surface is treated with non-reactive protein, and the streptavidin or avidin solution is transferred from the lower end of the strip to the biotin- (Strept) avidin-biotin reaction with the first (strept) avidin-biotin reaction, and then the biotin-antibody polymer solution is sequentially delivered to the second (strept) avidin-biotin reaction Lt; RTI ID = 0.0 > immobilized < / RTI > 제1항에 있어서, 면역 스트립으로 사용되는 멤브레인은 니트로셀룰로오즈, 글라스 파이버 또는 나일론과 폴리비닐리덴 플루오라이드(PVDF)등 합성수지로소 세공크기가 0.1~20um인 재질임을 특징으로 하는 면역 멤브레인 스트립의 제조방법.The method according to claim 1, wherein the membrane used as the immunostrip is a material such as nitrocellulose, glass fiber, nylon and polyvinylidene fluoride (PVDF) having a micropore size of 0.1 to 20 μm. Way. 제1항에 있어서, 비오틴-비반응성 단백질 종합체는 관능그룹을 갖는 비오틴과 보바인 세럼 알부민, 카제인 또는 젤라틴과 같은 측정 항원-항체 반응에 관여하지 않은 단백질로 합성되며, 단백질 한 분자 당 반응된 비오틴의 분자수가 2이상임을 특징으로 하는 면역 멤브레인 스트립이 제조방법.The biotin-non-reactive protein complex according to claim 1, wherein the biotin-non-reactive protein complex is synthesized as a protein not involved in a measurement antigen-antibody reaction such as biotin having a functional group and bovine serum albumin, casein or gelatin, Wherein the number of biotin molecules is 2 or more. 제1항에 있어서, 멤브레인 일정지역에 물리흡착에 의한 비오틴-단백질 중합체의 부동화시 비오틴-비반응성 단백질 중합제의 물리흡작 시 농도는 0.1~10mg/mL이고,중합제 용액을 멤브레인 표면에 가한 후 100%의 습도가 유지되는 밀폐된 공간에서 10분~1시간 배양하는 것임을 특징으로 하는 면역 멤브레인 스트립의 제조방법.The method according to claim 1, wherein the biotin-non-reactive protein polymer is physically adsorbed at a concentration of 0.1 to 10 mg / mL when the biotin-protein polymer is immobilized by physical adsorption in a predetermined region of the membrane, Wherein the culture medium is cultured in an airtight space maintained at 100% humidity for 10 minutes to 1 hour. 제1항에 있어서, 세척시 제조된 면역 멤브레인 스트립은 0.1~1%의 트윈-20과 0.1~5%의 카제인 등 비반응성 단백질이 포함된 완충용액에 담아 5~60분간 배양한 후 중성완충액으로 세척함을 특징으로 하는 면역 멤브레인 스트립이 제조방법.2. The method according to claim 1, wherein the immune membrane strip prepared in the washing step is incubated for 5 to 60 minutes in a buffer solution containing non-reactive proteins such as 0.1 to 1% of Tween-20 and 0.1 to 5% of casein, ≪ RTI ID = 0.0 > 1, < / RTI > 제1항에 있어서, 스트렙트아비딘 또는 아비딘 용액을 보바인 세럼 알부민 또는 산성도 6~8을 지닌 완충 용액으로 희석된 스트렙토아비딘 또는 아비딘 용액임을 하는 면역 면역 멤브레인 스트립의 제조방법.3. The method of claim 1, wherein the streptavidin or avidin solution is a streptavidin or avidin solution diluted with bovine serum albumin or a buffer solution having an acidity of 6-8. 제1항의 방법에 따라 제조된 면역 멤브레인 방법.A method of immunomembrane prepared according to the method of claim 1. 제7항의 면역 멤브레인 스트립의 임신 배란 알레르기 등의 가정용 진단시약, ADIS바이러스 진단, 간염 바이러스 진단, 식중독 진단 등의 전염성 질환등의 식품 및 환경 진단 또는 동식물류에 대한 면역학적 진단물질로 사용하는 방법.A method for use as an immunological diagnostic substance for food or environment diagnosis such as a domestic diagnostic reagent such as the pregnancy ovarian allergy of the immunostatic strip of claim 7, an infectious disease such as ADIS virus diagnosis, hepatitis virus diagnosis and food poisoning diagnosis, ※ 참고사항 : 최초 출원 내용에 의하여 공개하는 것임.※ Note: It is disclosed by the contents of the first application.
KR1019960002485A 1996-02-02 1996-02-02 A process for preparing immuno membrane strip KR100416490B1 (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101030640B1 (en) * 2009-02-13 2011-04-20 김보성 Manufacturing apparatus for allergy test strip

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WO2021167347A1 (en) 2020-02-17 2021-08-26 주식회사 아모그린텍 Method for manufacturing fiber assembly for providing binding surface to bio-substance and fiber assembly, manufactured thereby, for providing binding surface to bio-substance

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US4200690A (en) * 1976-12-16 1980-04-29 Millipore Corporation Immunoassay with membrane immobilized antibody
JPH03139700A (en) * 1989-10-25 1991-06-13 Sony Corp Audio signal reproducing device

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101030640B1 (en) * 2009-02-13 2011-04-20 김보성 Manufacturing apparatus for allergy test strip

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