KR970062023A - Medium composition for mycelial culture of shellinus linteus and method of cultivating mycelial using same - Google Patents

Medium composition for mycelial culture of shellinus linteus and method of cultivating mycelial using same Download PDF

Info

Publication number
KR970062023A
KR970062023A KR1019970024618A KR19970024618A KR970062023A KR 970062023 A KR970062023 A KR 970062023A KR 1019970024618 A KR1019970024618 A KR 1019970024618A KR 19970024618 A KR19970024618 A KR 19970024618A KR 970062023 A KR970062023 A KR 970062023A
Authority
KR
South Korea
Prior art keywords
medium
culture
mycelium
situational
brown rice
Prior art date
Application number
KR1019970024618A
Other languages
Korean (ko)
Other versions
KR100218897B1 (en
Inventor
조성인
김철
Original Assignee
조성인
김철
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 조성인, 김철 filed Critical 조성인
Priority to KR1019970024618A priority Critical patent/KR100218897B1/en
Publication of KR970062023A publication Critical patent/KR970062023A/en
Application granted granted Critical
Publication of KR100218897B1 publication Critical patent/KR100218897B1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Virology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Mushroom Cultivation (AREA)

Abstract

본 발명은 현미, 볶은현미, 보리, 보리분 또는 볶은 통보리중 적어도 1종 40~60wt%와 지하수 40~60wt%로 구성되어지되 종균배양용 병에 병입한 후 고압멸균기를 이용하여 15lb/㎤의 증기압(120~121℃)에서 50~60분간 가열하여 멸균하고 냉각한 배지에 자연산 상황의 자실체에서 조직을 분리하고 뽕나무 추출 배지에서 계대배양한 후 상기 배지와 같은 배지에서 종배양한 종배양액을 접종하여 25℃±1의 온도에서 25~30일간 배양함을 특징으로 하는 상황균사체 배양용 배지 조성물 및 이를 이용한 상황균사체의 배양방법에 관한 것으로, 쉽게 얻을 수 있는 곡물류를 배지성분으로 사용하므로서 상황균사체를 고밀도를 용이하게 얻을 수 있을 뿐만아니라 배양완료 후 배지성분과 더불어 상황균사체를 모두 이용할 수 있으므로 상황균사체의 추출정제가 필요없이 경제적이며 균사체 배양공정이 간단한 효과가 있다.The present invention is composed of at least one of 40 ~ 60wt% and ground water 40 ~ 60wt% of brown rice, roasted brown rice, barley, barley flour or roasted algae, but after bottling into a seed culture bottle using a high pressure sterilizer of 15lb / cm3 Sterilize by heating for 50 to 60 minutes at steam pressure (120 ~ 121 ℃), sterilized and cooled medium is separated from the fruiting body of the natural state, subcultured in mulberry extract medium and seeded with the seed culture cultured in the same medium as the medium The present invention relates to a culture medium composition for cultured situational mycelium and a method of cultivating situational mycelium using the same, characterized by culturing at a temperature of 25 ° C ± 1 for 25 to 30 days. Not only can high density be easily obtained, but also the situational mycelium can be used together with the medium components after the completion of culture, so it is economical without the need for extraction and purification of the situational mycelium. The mycelium culture process has a simple effect.

Description

상황(Phellinus linteus)균사체 배양용 배지조성을 및 이를 이용한 상화균사체의 배양방법Composition of medium for mycelial culture of shellinus linteus and culture method of cultured mycelia using same

본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음Since this is an open matter, no full text was included.

Claims (2)

현미, 볶은현미, 보리, 보리분 또는 볶은 통조리중 적어도 1종 40~60wt%와 지하수 40~60wt%로 구성되어지되 종균배양용 병에 병입한 후 고압멸균한 것을 특징으로 하는 상황균사체 배양용 배지 조성물.Brown rice, roasted brown rice, barley, barley flour or at least one of the roasted canned food consisting of 40 ~ 60wt% and ground water 40 ~ 60wt%, but after the high-pressure sterilization characterized in that bottled in the spawn culture bottle Medium composition. 자연산 상황의 자실체에서 조직을 분리한 다음, 뽕나무 고목을 재료로 하는 고형배지에서 정지배양의 방법으로 계대배양하고, 250㎖의 삼각플라스크에 현미50g과 50cc의 물을 주입후 고압살균(121℃/1.2㎏/50분)을 실시하고 냉각시킨 다음, 상기 계대배양액된 균사체를 무균상자 또는 무균실에서 접종하여 종배양한 종배양액을 제1항의 조성물로 조성된 배지에 접종하여 배양함을 특징으로 하는 상황균사체의 배양방법.After separating the tissue from the fruiting body of the natural situation, subcultured by the method of stationary culture in a solid medium made of mulberry wood, and injecting 50g of brown rice and 50cc of water into a 250ml Erlenmeyer flask and autoclaving it at 121 ℃ / 1.2 kg / 50 minutes) and cooled, and then inoculated with the subcultured mycelium in a sterile box or a sterile chamber, and incubated by inoculating the culture medium inoculated in a medium composed of the composition of claim 1 Culture method of mycelium. ※ 참고사항 : 최초출현 내용에 의하여 공개하는 것임.※ Note: It is to be disclosed based on the initial appearance.
KR1019970024618A 1997-06-13 1997-06-13 Medium for phellinus linteus culture and culturing method of phellinus linteus with the medium KR100218897B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
KR1019970024618A KR100218897B1 (en) 1997-06-13 1997-06-13 Medium for phellinus linteus culture and culturing method of phellinus linteus with the medium

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR1019970024618A KR100218897B1 (en) 1997-06-13 1997-06-13 Medium for phellinus linteus culture and culturing method of phellinus linteus with the medium

Publications (2)

Publication Number Publication Date
KR970062023A true KR970062023A (en) 1997-09-12
KR100218897B1 KR100218897B1 (en) 1999-10-01

Family

ID=19509506

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1019970024618A KR100218897B1 (en) 1997-06-13 1997-06-13 Medium for phellinus linteus culture and culturing method of phellinus linteus with the medium

Country Status (1)

Country Link
KR (1) KR100218897B1 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20030032677A (en) * 2001-10-19 2003-04-26 김안식 Method of culturing Phellinus linteus by seed fungus which incubated in culture fluid
KR102477517B1 (en) 2020-07-15 2022-12-15 이재갑 Complexed culture method of 6 types of mushroom mycelium using gastrodia elata blume medium
CN113755348A (en) * 2021-10-27 2021-12-07 重庆市蚕业科学技术研究院 Separation and purification method of wild phellinus igniarius strain

Also Published As

Publication number Publication date
KR100218897B1 (en) 1999-10-01

Similar Documents

Publication Publication Date Title
US11343978B2 (en) Methods for the production and use of myceliated high protein food compositions
JP2006296245A (en) Fermented ginger juice, method for producing the same, and food and drink
CN101702984A (en) Phellinus igniarius mycelium liquid fermentation method by utilizing fungal elicitor for improving yield of flavone of phellinus igniarius
KR970062023A (en) Medium composition for mycelial culture of shellinus linteus and method of cultivating mycelial using same
CN101503725B (en) Technique for improving purity of diphtheria toxoid
CN111869507A (en) Method for cultivating cordyceps sinensis by taking passion fruit as container
KR970021277A (en) Method of manufacturing ginseng fermented wine by yeast
CN110839795A (en) Preparation process of cistanche deserticola and golden cynomorium songaricum functional enzyme beverage
KR0133989B1 (en) Soy sauce using mushroom and method for production thereof
KR101435582B1 (en) Method for Producing Bacterial Cellulose with Gluconacetobacter sp. KCG326 Strain Using Optimal Media Composition
CN108967050A (en) A kind of preparation method of agaricus bisporus strain
CN114409826B (en) Method for extracting chitin from periplaneta americana dregs
JP2001269164A (en) Deer antler-shaped fruit body of ganoderma lucidum, and method for producing the same
CN106070780A (en) A kind of mushroom mycelium fermented tea and processing method thereof
CN109022212A (en) A kind of brewing method of dry type blistering loquat wine
JP3227430B2 (en) Method for producing eryngii mycelium extract
CA2163614A1 (en) Method of producing a taxane-type diterpene
KR20020093690A (en) Production of honey beverage
KR20110058928A (en) Scrubber Cultured Edible or Medicinal Mushrooms
KR20010067650A (en) Korean medicine agriculture a method
KR20160065434A (en) Citrus juices and juice from fruit such as utilizing mushroom mycelium extract medium manufacturing method
KR20210075348A (en) Manufacturing method of fermented tea
RU96105245A (en) METHOD OF I.K. TUTOV AND V.I.
JPS5742630A (en) Deodorization of "dokudami" a herb w(th bad smell)
KR970021278A (en) Method of preparing pumpkin liquor by yeast

Legal Events

Date Code Title Description
A201 Request for examination
G15R Request for early opening
N231 Notification of change of applicant
E902 Notification of reason for refusal
E701 Decision to grant or registration of patent right
GRNT Written decision to grant
FPAY Annual fee payment

Payment date: 20030520

Year of fee payment: 5

LAPS Lapse due to unpaid annual fee