KR970059274A - 수두 바이러스의 대량 생산 방법 - Google Patents

수두 바이러스의 대량 생산 방법 Download PDF

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KR970059274A
KR970059274A KR1019960001485A KR19960001485A KR970059274A KR 970059274 A KR970059274 A KR 970059274A KR 1019960001485 A KR1019960001485 A KR 1019960001485A KR 19960001485 A KR19960001485 A KR 19960001485A KR 970059274 A KR970059274 A KR 970059274A
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pox virus
chicken pox
infected cells
mass
chickenpox virus
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KR1019960001485A
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KR100434024B1 (ko
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김진환
윤성관
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성재갑
주식회사 Lg 화학
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0688—Cells from the lungs or the respiratory tract
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/16011—Herpesviridae
    • C12N2710/16711—Varicellovirus, e.g. human herpesvirus 3, Varicella Zoster, pseudorabies
    • C12N2710/16751—Methods of production or purification of viral material

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  • Health & Medical Sciences (AREA)
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  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
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  • Bioinformatics & Cheminformatics (AREA)
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  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Virology (AREA)
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  • Immunology (AREA)
  • Pulmonology (AREA)
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  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

본 발명은 수두 바이러스의 대량 생산 방법에 관한 것으로, 인간 태아의 폐 섬유 세포를 미립담체에 부착시켜 배양하고, 수두바이러스 감염 세포를 미립담체 상의 비감염 세포에 부착시켜 배양함으로써 수두 바이러스를 대량으로 생산할 수 있다.

Description

수두 바이러스의 대량 생산 방법.
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
제1도는 미립담체 상의 비감염 숙주세포에 수두 바이러스 세포를 부착시켰을 때 불량한 조건으로 인해 세포가 미립담체에서 모두 떨어진 상태를 현미경으로 관찰한 사진{×40, ×100}이며,
제3도는 피퓨전 방식으로 미립담체상에서 배양한 MCR-5 세포주의 배양일수에따른 세포수 및 수두 바이러스 역간의 변화 추이를 나타내는 그래프이고,
제5도는 피퓨전 방식으로 미립담체상에서 배양한 LBHEL 세포의 수두 바이러스의 감염 세포 역가(PFC)와 세포와 바이러스 역가(PFU)를 나타낸 그래프이다.

Claims (4)

  1. 인간 태아의 폐 섬유 세포를 미립담채에 부착시켜 배양하고, 수두 바이러스 감염 세포를 미립담채 상의 비감염 세포에 부착시켜 배양하는 것을 포함하는 수두 바이러스의 대량 생산방법.
  2. 제1항에 있어서, 상기 배양이 배지를 연속적으로 교환하는 퍼퓨전 방식으로 수행되는 것을 특징으로 하는 방법.
  3. 제1항에 있어서, 상기 배양 배지가 40내지 45밀리몰의 중탄산나트륨을 함유하는 것을 특징으로 하는 방법.
  4. 제2항에 있어서, 상기 배지의 교환이 0.5 내지 4VVD로 이루어지는 것을 특징으로 하는 방법.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019960001485A 1996-01-24 1996-01-24 수두바이러스의대량생산방법 KR100434024B1 (ko)

Priority Applications (1)

Application Number Priority Date Filing Date Title
KR1019960001485A KR100434024B1 (ko) 1996-01-24 1996-01-24 수두바이러스의대량생산방법

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR1019960001485A KR100434024B1 (ko) 1996-01-24 1996-01-24 수두바이러스의대량생산방법

Publications (2)

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KR970059274A true KR970059274A (ko) 1997-08-12
KR100434024B1 KR100434024B1 (ko) 2004-07-21

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103074304B (zh) * 2013-01-28 2016-01-13 江苏健安生物科技有限公司 高水平人二倍体细胞培养水痘-带状疱疹疫苗病毒方法

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2177060A1 (en) * 1993-12-21 1995-06-29 Philip J. Provost Thermostable varicella zoster virus
KR0135614B1 (ko) * 1994-11-23 1998-04-23 허영섭 약독화 수두 생바이러스 백신 및 그의 제조방법

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