KR970021285A - Preparation method of Lactic acid bacteria freeze-dried cells using reduced whey base-freeze-drying protector - Google Patents

Preparation method of Lactic acid bacteria freeze-dried cells using reduced whey base-freeze-drying protector Download PDF

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KR970021285A
KR970021285A KR1019950035787A KR19950035787A KR970021285A KR 970021285 A KR970021285 A KR 970021285A KR 1019950035787 A KR1019950035787 A KR 1019950035787A KR 19950035787 A KR19950035787 A KR 19950035787A KR 970021285 A KR970021285 A KR 970021285A
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South Korea
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weight
lactic acid
freeze
parts
acid bacteria
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KR1019950035787A
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Korean (ko)
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KR0145384B1 (en
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서인영
윤상군
김경태
배형석
백영진
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이은선
주식회사 한국야쿠르트
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Abstract

1. 청구범위의 기재된 발명이 속한 기술분야1. TECHNICAL FIELD OF THE INVENTION

본 발명은 젖산균의 동결건조법에 관한 것이다.The present invention relates to a lyophilization method of lactic acid bacteria.

2. 발명이 해결하려고 하는 기술적 과제2. The technical problem to be solved by the invention

치즈 생산의 부산물인 유청이, 종래에 동결건조 보호제의 분산매로 널리 사용되어오던 탈지분유와 비교해 볼 때, 동결건조시 보다 향상된 안정성과 보존성을 제공한다는 사실을 기초로 하여, 환원 유청을 분산매로 한 새로운 조성의 동결건조 보호제를 사용한 젖산균 동결건조 균체의 제조방법을 제공하는데 본 발명의 목적이 있다.Whey, which is a byproduct of cheese production, provides better stability and preservation during lyophilization compared to skim milk powder, which has been widely used as a lyophilizer in the past. It is an object of the present invention to provide a method for producing lactic acid bacteria lyophilized cells using a lyophilized protective agent of a new composition.

3. 발명의 해결방법의 요지3. Summary of Solution to Invention

본 발명은 5-20중량부의 환원 유청용액에 1-20중량부의 폴리올, 0.1-0.5M의 아미노산, 0.1-10중량부의 고분자물질, 0.05-1중량부의 아스코르브산, 또는 이들의 혼합물이 동결건조 보조제로서 첨가되는 공정을 포함하는 환원 유청 기초-동결건조 보호제를 사용한 젖산균 동결건조 균체의 제조방법을 제공한다.The present invention is a 1-20 parts by weight of polyol, 0.1-0.5M amino acid, 0.1-10 parts by weight of a polymeric material, 0.05-1 parts by weight of ascorbic acid, or a mixture thereof in a 5-20 parts by weight of reducing whey solution It provides a method for producing lactic acid bacteria lyophilized cells using a reduced whey base-freeze-drying protective agent comprising a step added as.

4. 본 발명의 중요한 용도4. Important uses of the present invention

본 발명에 따른 제조방법으로 생존률 및 보존성이 우수한 젖산간균, 젖산구균 및 비피도박테리아의 동결건조균체를 제조할 수 있다.Lactobacillus bacillus, lactic acid cocci, and lyophilised bacteria of Bifidobacteria can be prepared by the production method according to the present invention.

Description

환원 유청 기초-동결건조 보호제를 사용한 젓산균 동결건조 균체의 제조방법Preparation method of Lactic acid bacteria freeze-dried cells using reduced whey base-freeze-drying protector

본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음Since this is an open matter, no full text was included.

Claims (5)

5-20중량부의 환원 유청용액이 1-20중량부의 폴리올, 0.1-0.5M의 아미노산, 0.1-10중량부의 고분자물질, 0.05-1중량부의 아스코르브산, 또는 이들의 혼합물이 동결건조 보조제로서 첨가되는 공정을 포함하는 환원 유청 기초-동결건조 보호제를 사용한 젖산균 동결건조 균체의 제조방법.5-20 parts by weight of reduced whey solution is added with 1-20 parts by weight of polyol, 0.1-0.5 M amino acid, 0.1-10 parts by weight of polymeric material, 0.05-1 part by weight of ascorbic acid, or a mixture thereof as a lyophilization aid. A method for producing lactic acid bacteria lyophilized cells using a reduced whey base-freeze-drying protective agent comprising a step. 제1항에 있어서, 상기 동결건조 보호제는 구연산, 수산화나트륨, 제1인산나트륨, 제2인산나트륨, 제1인산칼륨, 제2인산칼륨, 탄산나트륨, 중탄산나트륨, 탄산칼슘 및 이들의 혼합물로부터 선택된 1종의 pH 조정제를 사용하여 pH 5.0-9.0으로 조정되는 것을 특징으로 하는 환원 유청 기초-동결건조 보호제를 사용한 젖산균 동결건조 균체의 제조방법.The method of claim 1, wherein the lyophilizing protector is selected from citric acid, sodium hydroxide, sodium monophosphate, sodium diphosphate, potassium monophosphate, dipotassium phosphate, sodium carbonate, sodium bicarbonate, calcium carbonate and mixtures thereof. A method for producing lactic acid bacteria lyophilized cells using a reduced whey base-freeze-drying protector, characterized in that the pH is adjusted to 5.0-9.0 using a pH adjuster of the species. 제1항에 있어서, 상기 폴리올은 글리세롤, 아도니톨, 솔비톨, 및 이들의 혼합물로부터 선택되는 것을 특징으로 하는 환원 유청 기초-동결건조 보호제를 사용한 젖산균 동결건조 균체의 제조방법.The method of claim 1, wherein the polyol is selected from glycerol, adonitol, sorbitol, and mixtures thereof. 제1항에 있어서, 상기 아미노산 물질은 글리신, 글루타민산, 및 이들의 혼합물로부터 선택되는 것을 특징으로 하는 환원 유청 기초-동결건조 보호제를 사용한 젖산균 동결건조 균체의 제조방법.The method of claim 1, wherein the amino acid material is selected from glycine, glutamic acid, and mixtures thereof. 제1항에 있어서, 상기 고분자 물질은 젤라틴, 카르복시 메틸 셀룰로오즈, 셀룰로오즈, 녹말, 아르긴산, 한천, 구아, 및 이들의 혼합물로부터 선택되는 것을 특징으로 하는 환원 유청 기초-동결건조 보호제를 사용한 젖산균 동결건조 균체의 제조방법.The method of claim 1, wherein the polymer material is selected from gelatin, carboxy methyl cellulose, cellulose, starch, arginic acid, agar, guar, and mixtures thereof. Production method of the cells. ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.※ Note: The disclosure is based on the initial application.
KR1019950035787A 1995-10-17 1995-10-17 Reduction Whey Basics-Method for Preparation of Lactic Acid Bacteria Freeze-Dried Cells Using Lyophilized Protective Agent KR0145384B1 (en)

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