KR970010961A - Culture medium and culture method of virus and preparation method of hepatitis vaccine using the same - Google Patents

Culture medium and culture method of virus and preparation method of hepatitis vaccine using the same Download PDF

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Publication number
KR970010961A
KR970010961A KR1019950024037A KR19950024037A KR970010961A KR 970010961 A KR970010961 A KR 970010961A KR 1019950024037 A KR1019950024037 A KR 1019950024037A KR 19950024037 A KR19950024037 A KR 19950024037A KR 970010961 A KR970010961 A KR 970010961A
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South Korea
Prior art keywords
liver tissue
hepatitis virus
virus
culture
infected
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KR1019950024037A
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Korean (ko)
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백계형
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백계형
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Priority to KR1019950024037A priority Critical patent/KR970010961A/en
Publication of KR970010961A publication Critical patent/KR970010961A/en

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  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

본 발명은 동물의 간조직을 이용하여 in vitro에서 간염 바이러스를 다량 배양하는 방법과 이로부터 간염 바이러스 백신을 제조하는 방법 및 제조된 백신에 관한 것이다.The present invention relates to a method of culturing a large amount of hepatitis virus in vitro using liver tissue of an animal, a method of preparing a hepatitis virus vaccine therefrom, and a vaccine prepared.

더욱 상세하게는, 본 발명은 웨이머스 엠비(Waymouth MB)725/1분말배지, 10%소 태아 혈청, 2.2%중탄산 나트륨 완충액, 25mM D-포도당, 인슐린 1~50㎍/cc 및 항생제를 포함하여 구성된 배지를 함유하고 있는 회전식 배양관의 사각기둥 메쉬상에,m 감염 바이러스에 감염된 간조직편을 올려 놓고 1차 배양한 수, 감염된 간조직편을 제거하고, 얻어진 배양액에 정상 간조직편을 2차 배양하는 것을 특징으로 하는 간염 바이러스의 배양방법에 관한 것이며, 이에 의해 생산된 바이러스를 이용하여 제조되는 간염 바이러스 백신에 관한 것이다.More specifically, the present invention includes a Waymouth MB 725/1 powder medium, 10% fetal bovine serum, 2.2% sodium bicarbonate buffer, 25 mM D-glucose, 1-50 μg / cc insulin and antibiotics. On the square-column mesh of the rotary culture tube containing the constructed medium, m-infected virus-tissue liver tissue pieces were placed on the primary culture, infected liver tissue fragments were removed, and normal liver tissue fragments were secondary cultured in the obtained culture solution. It relates to a method for culturing hepatitis virus, characterized in that hepatitis virus vaccine produced using the virus produced thereby.

따라서, 본 발명에 의하여 불가능했던 간염 바이러스를 in vitro에서 대량으로 배양할 수 있으며, 이를 이용하여 항체 형성 효율이 높은 간염 바이러스 백신을 제조할 수 있다.Therefore, the hepatitis virus, which was not possible by the present invention, can be cultured in a large amount in vitro, and a hepatitis virus vaccine having high antibody formation efficiency can be prepared using the same.

Description

바이러스의 배양배지 및 배양방법과 이를 이용한 간염백신의 제조방법Culture medium and culture method of virus and method of manufacturing hepatitis vaccine using the same

본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음As this is a public information case, the full text was not included.

제1도는 본 발명의 바이러스 배양방법에 사용되는 배양기의 단면 개략도이다.1 is a schematic cross-sectional view of an incubator used in the virus culture method of the present invention.

Claims (3)

웨이머스 엠비(Waymouth MB)725/1분말배지, 10%소 태아 혈청, 2.2%중탄산 나트륨 완충액, 25mM D-포도당, 인슐린 1~50㎍/cc 및 항생제를 포함하여 구성된 배지를 함유하고 있는 회전식 배양관의 사각기둥 메쉬상에,m 감염 바이러스에 감염된 간조직편을 올려 놓고 1차 배양한 후, 감염된 간조직편을 제거하고, 얻어진 배양액에 정상 간조직편을 2차 배양하는 것을 특징으로 하는 간염 바이러스의 배양방법Waymouth MB Rotated culture containing medium containing 725/1 powder medium, 10% fetal bovine serum, 2.2% sodium bicarbonate buffer, 25 mM D-glucose, 1-50 μg / cc insulin and antibiotics Cultured hepatitis virus, characterized in that the m-infected virus-infected liver tissue piece is placed on the square-column mesh of the tube, followed by primary culture, the infected liver tissue piece is removed, and the normal liver tissue piece is secondary cultured in the obtained culture solution. Way 제1항에 있어서, 상기 정상 간조직편으로 쥐(rat), 생쥐(mouse), 개, 닭, 또는 개구리의 간조직편을 사용하는 것을 특징으로 하는 간염 바이러스의 배양방법.The method of culturing hepatitis virus according to claim 1, wherein liver tissue pieces of rats, mice, dogs, chickens, or frogs are used as the normal liver tissue pieces. 제1항의 방법에 따라 생산된 바이러스를 이용하여 제조되는 간염 바이러스 백신.Hepatitis virus vaccine prepared using a virus produced according to the method of claim 1. ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.※ Note: The disclosure is based on the initial application.
KR1019950024037A 1995-08-04 1995-08-04 Culture medium and culture method of virus and preparation method of hepatitis vaccine using the same KR970010961A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
KR1019950024037A KR970010961A (en) 1995-08-04 1995-08-04 Culture medium and culture method of virus and preparation method of hepatitis vaccine using the same

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR1019950024037A KR970010961A (en) 1995-08-04 1995-08-04 Culture medium and culture method of virus and preparation method of hepatitis vaccine using the same

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KR970010961A true KR970010961A (en) 1997-03-27

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