KR970010961A - Culture medium and culture method of virus and preparation method of hepatitis vaccine using the same - Google Patents
Culture medium and culture method of virus and preparation method of hepatitis vaccine using the same Download PDFInfo
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- KR970010961A KR970010961A KR1019950024037A KR19950024037A KR970010961A KR 970010961 A KR970010961 A KR 970010961A KR 1019950024037 A KR1019950024037 A KR 1019950024037A KR 19950024037 A KR19950024037 A KR 19950024037A KR 970010961 A KR970010961 A KR 970010961A
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- liver tissue
- hepatitis virus
- virus
- culture
- infected
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- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
본 발명은 동물의 간조직을 이용하여 in vitro에서 간염 바이러스를 다량 배양하는 방법과 이로부터 간염 바이러스 백신을 제조하는 방법 및 제조된 백신에 관한 것이다.The present invention relates to a method of culturing a large amount of hepatitis virus in vitro using liver tissue of an animal, a method of preparing a hepatitis virus vaccine therefrom, and a vaccine prepared.
더욱 상세하게는, 본 발명은 웨이머스 엠비(Waymouth MB)725/1분말배지, 10%소 태아 혈청, 2.2%중탄산 나트륨 완충액, 25mM D-포도당, 인슐린 1~50㎍/cc 및 항생제를 포함하여 구성된 배지를 함유하고 있는 회전식 배양관의 사각기둥 메쉬상에,m 감염 바이러스에 감염된 간조직편을 올려 놓고 1차 배양한 수, 감염된 간조직편을 제거하고, 얻어진 배양액에 정상 간조직편을 2차 배양하는 것을 특징으로 하는 간염 바이러스의 배양방법에 관한 것이며, 이에 의해 생산된 바이러스를 이용하여 제조되는 간염 바이러스 백신에 관한 것이다.More specifically, the present invention includes a Waymouth MB 725/1 powder medium, 10% fetal bovine serum, 2.2% sodium bicarbonate buffer, 25 mM D-glucose, 1-50 μg / cc insulin and antibiotics. On the square-column mesh of the rotary culture tube containing the constructed medium, m-infected virus-tissue liver tissue pieces were placed on the primary culture, infected liver tissue fragments were removed, and normal liver tissue fragments were secondary cultured in the obtained culture solution. It relates to a method for culturing hepatitis virus, characterized in that hepatitis virus vaccine produced using the virus produced thereby.
따라서, 본 발명에 의하여 불가능했던 간염 바이러스를 in vitro에서 대량으로 배양할 수 있으며, 이를 이용하여 항체 형성 효율이 높은 간염 바이러스 백신을 제조할 수 있다.Therefore, the hepatitis virus, which was not possible by the present invention, can be cultured in a large amount in vitro, and a hepatitis virus vaccine having high antibody formation efficiency can be prepared using the same.
Description
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음As this is a public information case, the full text was not included.
제1도는 본 발명의 바이러스 배양방법에 사용되는 배양기의 단면 개략도이다.1 is a schematic cross-sectional view of an incubator used in the virus culture method of the present invention.
Claims (3)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
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KR1019950024037A KR970010961A (en) | 1995-08-04 | 1995-08-04 | Culture medium and culture method of virus and preparation method of hepatitis vaccine using the same |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
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KR1019950024037A KR970010961A (en) | 1995-08-04 | 1995-08-04 | Culture medium and culture method of virus and preparation method of hepatitis vaccine using the same |
Publications (1)
Publication Number | Publication Date |
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KR970010961A true KR970010961A (en) | 1997-03-27 |
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Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
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KR1019950024037A KR970010961A (en) | 1995-08-04 | 1995-08-04 | Culture medium and culture method of virus and preparation method of hepatitis vaccine using the same |
Country Status (1)
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KR (1) | KR970010961A (en) |
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1995
- 1995-08-04 KR KR1019950024037A patent/KR970010961A/en not_active Application Discontinuation
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