KR970001534A - Pseudomonas genus bacterium KAC-1 producing glutaryl 7-aminocephalosporanic acid acylates - Google Patents

Pseudomonas genus bacterium KAC-1 producing glutaryl 7-aminocephalosporanic acid acylates Download PDF

Info

Publication number
KR970001534A
KR970001534A KR1019950018730A KR19950018730A KR970001534A KR 970001534 A KR970001534 A KR 970001534A KR 1019950018730 A KR1019950018730 A KR 1019950018730A KR 19950018730 A KR19950018730 A KR 19950018730A KR 970001534 A KR970001534 A KR 970001534A
Authority
KR
South Korea
Prior art keywords
aca
kac
pseudomonas genus
aminocephalosporanic acid
genus bacterium
Prior art date
Application number
KR1019950018730A
Other languages
Korean (ko)
Other versions
KR100232638B1 (en
Inventor
김정우
정구헌
이왕식
윤기홍
박장우
정경화
김대원
신한재
Original Assignee
김충환
주식회사 종근당
김은영
한국과학기술연구원
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 김충환, 주식회사 종근당, 김은영, 한국과학기술연구원 filed Critical 김충환
Priority to KR1019950018730A priority Critical patent/KR100232638B1/en
Publication of KR970001534A publication Critical patent/KR970001534A/en
Application granted granted Critical
Publication of KR100232638B1 publication Critical patent/KR100232638B1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
    • C12N9/80—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5) acting on amide bonds in linear amides (3.5.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P35/00—Preparation of compounds having a 5-thia-1-azabicyclo [4.2.0] octane ring system, e.g. cephalosporin
    • C12P35/04—Preparation of compounds having a 5-thia-1-azabicyclo [4.2.0] octane ring system, e.g. cephalosporin by acylation of the substituent in the 7 position
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/38—Pseudomonas
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y305/00—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5)
    • C12Y305/01—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5) in linear amides (3.5.1)
    • C12Y305/01093—Glutaryl-7-aminocephalosporanic-acid acylase (3.5.1.93)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

본 발명은 글루타릴 7-아미노세팔로스포란산(GL 7-ACA) 아실레이즈를 생산하는 신규의 슈도모나스속의 세균 KAC-1(수탁번호 KCTC 8668P)에 관한 것이다. 신규의 GL 7-ACA 아실레이즈는 GL 7-ACA를 7-ACA로 전환시키는데 이용된다. 7-ACA는 세파로스포린계 항생제의 중간원료물질로 널리 쓰이고 있다.The present invention relates to a novel Pseudomonas bacterium KAC-1 (Accession No. KCTC 8668P) that produces glutaryl 7-aminocephalosporranic acid (GL 7-ACA) acylates. The new GL 7-ACA acylate is used to convert the GL 7-ACA to 7-ACA. 7-ACA is widely used as an intermediate material for cephalosporin antibiotics.

Description

글루타릴 7-아미노세팔로스포란산 아실레이즈를 생산하는 슈도모나스 속 세균 KAC-1Pseudomonas genus bacterium KAC-1 producing glutaryl 7-aminocephalosporanic acid acylates

본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음Since this is an open matter, no full text was included.

제1도는 본 발명의 신균주 슈도모나스(Pseudomonas)속 KAC-1의 전자현미경 사진이다. 제2도는 본 발명의 신규 글루타릴 7-아미노세팔로스포란산(Glutaryl 7-aminocephalosporanic acid; GL 7-ACA) 아실레이즈가 GL 7-ACA를 7-ACA로 전환시키는 반응에서 반응산물인 7-ACA와 글루타릴산이 효소의 가수분해 반응을 저해하는 정도를 나타낸 그래프이다, 제3도는 본 발명의 신규 글루타릴 7-아미노세팔로스포란산 아실레이즈 효소의 합성반응을 나타낸 그래프이다.1 is an electron micrograph of KAC-1 genus Pseudomonas of the present invention. FIG. 2 shows the reaction product 7 in the reaction of the novel glutaryl 7-aminocephalosporanic acid (GL 7-ACA) acylase converts GL 7-ACA to 7-ACA. It is a graph showing the degree to which -ACA and glutaric acid inhibit the hydrolysis reaction of the enzyme. FIG. 3 is a graph showing the synthesis reaction of the novel glutaryl 7-aminocephalosporanic acid acylase enzyme of the present invention.

Claims (4)

신규 분리한 슈도모나스 속 KAC-1 균주(수탁번호 KCTC 8668P).Newly isolated Pseudomonas genus KAC-1 strain (Accession No. KCTC 8668P). 제1항에 따른 신규 슈도모나스 속 KAC-1 또는 그 유전물질의 발현에 의해서 생산되는 것을 특징으로 하는 GL 7-ACA 아실레이즈 효소.GL 7-ACA acylase enzyme, characterized in that produced by the expression of the novel Pseudomonas genus KAC-1 or its genetic material. 제2항의 효소를 이용하여 GL 7-ACA 또는 그 유도체에서 7-ACA 또는 그 유도체를 제조하는 방법.A method for preparing 7-ACA or a derivative thereof from GL 7-ACA or a derivative thereof using the enzyme of claim 2. 제2항의 효소를 이용하여 7-ACA 또는 7-ADCA를 모핵으로 하는 세팔로스포린계 항생제에서 7번 곁사슬의 글루타릴산을 전이시키는 방법.A method of transferring the 7th side chain glutaric acid from a cephalosporin-based antibiotic using the enzyme of claim 2 as the mother nucleus of 7-ACA or 7-ADCA. ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.※ Note: The disclosure is based on the initial application.
KR1019950018730A 1995-06-30 1995-06-30 Novel pseudomonas sp. kac-1 which produce gl-7-aca acylase KR100232638B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
KR1019950018730A KR100232638B1 (en) 1995-06-30 1995-06-30 Novel pseudomonas sp. kac-1 which produce gl-7-aca acylase

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR1019950018730A KR100232638B1 (en) 1995-06-30 1995-06-30 Novel pseudomonas sp. kac-1 which produce gl-7-aca acylase

Publications (2)

Publication Number Publication Date
KR970001534A true KR970001534A (en) 1997-01-24
KR100232638B1 KR100232638B1 (en) 1999-12-01

Family

ID=19419160

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1019950018730A KR100232638B1 (en) 1995-06-30 1995-06-30 Novel pseudomonas sp. kac-1 which produce gl-7-aca acylase

Country Status (1)

Country Link
KR (1) KR100232638B1 (en)

Also Published As

Publication number Publication date
KR100232638B1 (en) 1999-12-01

Similar Documents

Publication Publication Date Title
Sonawane Enzymatic modifications of cephalosporins by cephalosporin acylase and other enzymes
KR910018549A (en) Cephalosporin acetylhydrolase gene and protein encoded by this gene
CA2077921A1 (en) Bioprocess for preparing 7-adca
WO2000037671A3 (en) Improved in vivo production of cephalosporins
AU2001243338A1 (en) High purity maltose process and products
MXPA04006304A (en) Process for the preparation of a beta-lactam antibiotic with mutated penicillin acylase.
AU2701692A (en) Novel bioprocesses for preparing 7-aca and 7-adac
KR930002377A (en) Gellan rubber for non-soft gels
AU5881701A (en) Novel cyclodextrin glucanotransferase, process for producing the same and process for producing cyclodextrin by using this enzyme
Gröger et al. Industrial landmarks in the development of sustainable production processes for the β-lactam antibiotic key intermediate 7-aminocephalosporanic acid (7-ACA)
US3239394A (en) Process for producing 7-amino-cephalosporanic acid
US3912589A (en) Preparation of cephalosporin compounds
US4774179A (en) Process for preparing a 7-aminocephalosporanic acid compound
DE69412352T2 (en) Streptococcus zooepidemicus mutant strain for the production of hyaluronic acid
GB9216759D0 (en) Process for the production of 7-amino thiazolyl cephalosporins
KR970001534A (en) Pseudomonas genus bacterium KAC-1 producing glutaryl 7-aminocephalosporanic acid acylates
GB2323361A (en) Improved process for the production of semi-synthetic cephalosporins via expandase activity on penicillin G
KR930700646A (en) σ-cyclodextrin glycosyl trans ferase
EP0818469A3 (en) Branched cyclodextrins and method for producing them
KR960041357A (en) Microorganisms, uses thereof, and methods for preparing L-α-amino acids using the same
AU4846196A (en) Process for the recovery of cephalexin
WO1996016174A3 (en) Cephalosporin c amidohydrolase, production and use
US5332663A (en) Process for the enzymatic preparation of 7-amino-cephalosporanic acids
HU217106B (en) Process for enzymatic acylation of 3-hydroxymethyl cephalosporins
Frederiksen et al. Conversion of Cephalosporin-C into 7-phenoxy-acetamido-cephalosporanic acid by acyltransferase of mutants of Penicillium chrysogenum

Legal Events

Date Code Title Description
AMND Amendment
A201 Request for examination
E902 Notification of reason for refusal
E601 Decision to refuse application
J201 Request for trial against refusal decision
B701 Decision to grant
GRNT Written decision to grant
FPAY Annual fee payment

Payment date: 20050824

Year of fee payment: 7

LAPS Lapse due to unpaid annual fee