KR960701986A - VIABLE BACTERIA - Google Patents

VIABLE BACTERIA

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Publication number
KR960701986A
KR960701986A KR1019950704675A KR19950704675A KR960701986A KR 960701986 A KR960701986 A KR 960701986A KR 1019950704675 A KR1019950704675 A KR 1019950704675A KR 19950704675 A KR19950704675 A KR 19950704675A KR 960701986 A KR960701986 A KR 960701986A
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KR
South Korea
Prior art keywords
cells
composition
matrix
polyhydroxy compound
microbial cells
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KR1019950704675A
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Korean (ko)
Inventor
데이비드 커크 로드햄
존 버네트 캔트웰
Original Assignee
비자야 쿠마리 말리페디
제네카 리미티드
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Application filed by 비자야 쿠마리 말리페디, 제네카 리미티드 filed Critical 비자야 쿠마리 말리페디
Publication of KR960701986A publication Critical patent/KR960701986A/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/04—Preserving or maintaining viable microorganisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
    • C12N11/04—Enzymes or microbial cells immobilised on or in an organic carrier entrapped within the carrier, e.g. gel or hollow fibres

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Medicinal Chemistry (AREA)
  • Dispersion Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Materials For Medical Uses (AREA)

Abstract

본 발명은 기포구조가 붕괴된 매트릭스내 현탁된 생장정지 상태(stasis state)의 건조 조성물의 형태로서 저장에 안정한 미생물 세포에 관한다.The present invention relates to microbial cells that are stable for storage in the form of a dry composition of suspended stasis state in a matrix in which the bubble structure has collapsed.

본 조성물은 예를 들어, 슈도모나스 플루오레슨스(Pseudomonas f1uorescens)와 같은 그람음성 박테리아세포를 매트릭스가 유도되는 폴리하이드록시 화합물, 바람직하게는 당류로 구성되는 수성 조성물과 혼합시키는 단계; 및 폴리하이드록시 화합물의 점성 흐름이 일어나서 매트릭스의 기포 구조가 붕괴되나 세포는 지나치게 손상되지 않도록 하는 조건하에서 상기 혼합물을 건조시키는 단계로 제조한다. 매트릭스의 유리 전이 온도는 이상적으로는 조성물의 저장온도 이상이다.The composition comprises, for example, mixing a Gram-negative bacterial cell such as Pseudomonas f1uorescens with an aqueous composition consisting of a matrix-derived polyhydroxy compound, preferably a saccharide; And drying the mixture under conditions such that a viscous flow of the polyhydroxy compound occurs such that the bubble structure of the matrix is disrupted but the cells are not excessively damaged. The glass transition temperature of the matrix is ideally above the storage temperature of the composition.

Description

생존 가능한 박테리아(VIABLE BACTERIA)VIABLE BACTERIA

본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음Since this is an open matter, no full text was included.

제1도는 물 또는 0.04M MgSO4로 동결 건조시킨 경우 이노시톨(첨가제)농도와 슈도모나스 풀루오레슨스의 생존 가능성 변화를 그래프의 형태로 예시하고 있다.Figure 1 illustrates, in graph form, the change in viability of inositol (additive) and Pseudomonas pullulose lessons when freeze-dried with water or 0.04 M MgSO 4 .

수직 축은 ppb로 나타낸 생존 가능성이고(즉 1E+09=109ppb, 1E+08=108ppb등) 수평 축은 샘플당 밀리그램으로 나타낸 첨가제의 농도이다.The vertical axis is the viability in ppb (ie 1E + 09 = 10 9 ppb, 1E + 08 = 10 8 ppb, etc.) and the horizontal axis is the concentration of the additive in milligrams per sample.

그래프 상의 흑색 정사각형(■)은 마그네슘 설페이트내 이노시톨을 플롯한 것이고 원(０)은 수중 이노시톨을 플롯한 것이다.The black square (■) on the graph plots inositol in magnesium sulfate and the circle (0) plots inositol in water.

제2도 내지 제6도는 단당류의 농도와 동결 건조시킨 슈도모나스 플루오레슨스의 생존 가능성 변화를 그래프의 형태로 예시하고 있다.2 through 6 illustrate the change in viability of monosaccharide concentration and freeze-dried Pseudomonas fluorescence in a graphical form.

Claims (13)

기포구조가 붕괴된(collapsed)매트릭스내에 현탁된 생장정지 상태(stasis state)의 미생물 세포로 구성되는 안정화된 건조 조성물.A stabilized dry composition consisting of microbial cells in a stasis state suspended in a matrix in which a bubble structure has collapsed. 제1항에 있어서, 미생물 세포가 박테리아 세포인 조성물.The composition of claim 1 wherein the microbial cells are bacterial cells. 제2항에 있어서, 박테리아 세포가 그람음성 박테리아 세포인 조성물.The composition of claim 2 wherein the bacterial cells are Gram-negative bacterial cells. 제3항에 있어서, 그람음성 세포가 슈돔나스 플루오레슨스(Pseudomonas fluorescens), 에스케리치아 콜리(Escherichia coli) 또는 근계(rhizosphere)에 결합된 박테리아인 조성물.The composition of claim 3 wherein the Gram-negative cells are bacteria bound to Pseudomonas fluorescens, Escherichia coli or rhizosphere. (A) : 매트릭스가 유도되는 물질로 구성되는 수성 조성물과 미생물 세포를 혼합시키는 단계; (B) : 상기물질의 점성 흐름이 일어나서 매트릭스의 기포구조가 붕괴되나 세포는 지나치게 손상되지 않도록 하는 조건하에 상기 혼합물을 건조시키는 단계로 구성되며, 매트릭스내 현탁된 생장정지 상태의 미생물 세포로 구성되는 안정화된 건조 조성물의 제조방법.(A): mixing the microbial cells with an aqueous composition consisting of a substance from which the matrix is derived; (B): drying the mixture under conditions such that a viscous flow of the material occurs to disrupt the bubble structure of the matrix but the cells are not excessively damaged, and are composed of microbial cells in suspended growth suspended in the matrix. Process for preparing a stabilized dry composition. 제5항에 있어서, 단계(B)에서 제조된 조성물을 더 건조시켜 매트릭스의 유리 전이 온도를 높임으로써 조성물이 더 넓은 저장 조건에서 안정화될 수 있도록 하는 방법.The method of claim 5, wherein the composition prepared in step (B) is further dried to increase the glass transition temperature of the matrix so that the composition can be stabilized at a wider storage condition. 제5항에 있어서, 단계(A)에서 제조된 혼합물내 미생물 세포의 농도가 104-1013/㎖인 방법.The method of claim 5, wherein the concentration of microbial cells in the mixture prepared in step (A) is 10 4 -10 13 / ml. 제7항에 있어서, 세포의 농도가 1010-1011/㎖인 방법.8. The method of claim 7, wherein the concentration of cells is 10 10 -10 11 / ml. 제5항에 있어서, 상기 방법의 단계(A)에서 미생물 세포와 혼합되는 물질이 폴리하이드록시 화합물인 방법.The method of claim 5, wherein the material mixed with the microbial cells in step (A) of the method is a polyhydroxy compound. 제9항에 있어서, 폴리하이드록시 화합물이 단망류인 방법.The method of claim 9, wherein the polyhydroxy compound is monocyclic. 제9항에 있어서, 단계(A)의 혼합물에 사용되는 폴리하이드록시 화합물의 농도가 세포 1010개당 10-1000㎎인 방법.The method of claim 9, wherein the concentration of the polyhydroxy compound used in the mixture of step (A) is 10-1000 mg per 10 10 cells. 제11항에 있어서, 폴리하이록시 화합물의 농도가 세포 1010개당 200-400㎎인 방법.The method of claim 11, wherein the concentration of polyhydroxy compound is 200-400 mg per 10 cells. 예상 저장 온도 이상의 유리 전이 온도를 갖는, 제5항에 따라 제조된 조성물.A composition prepared according to claim 5 having a glass transition temperature above the expected storage temperature. ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.※ Note: The disclosure is based on the initial application.
KR1019950704675A 1993-04-28 1994-04-18 VIABLE BACTERIA KR960701986A (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB9308734.4 1993-04-28
GB939308734A GB9308734D0 (en) 1993-04-28 1993-04-28 Viable bacteria
PCT/GB1994/000811 WO1994025564A1 (en) 1993-04-28 1994-04-18 Viable bacteria

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EP (1) EP0696316A1 (en)
JP (1) JPH08509374A (en)
KR (1) KR960701986A (en)
CN (1) CN1121731A (en)
AU (1) AU684072B2 (en)
BG (1) BG100105A (en)
BR (1) BR9406488A (en)
CA (1) CA2161220A1 (en)
CZ (1) CZ280595A3 (en)
GB (2) GB9308734D0 (en)
HU (1) HUT72846A (en)
NZ (1) NZ263867A (en)
PL (1) PL311297A1 (en)
SK (1) SK134695A3 (en)
WO (1) WO1994025564A1 (en)

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GB9619893D0 (en) * 1996-09-24 1996-11-06 Zeneca Ltd Novel composition
CA2312233A1 (en) * 1997-11-26 1999-06-03 Universal Preservation Technologies, Inc. Preservation of sensitive biological samples by vitrification
DE19819475A1 (en) * 1998-04-30 1999-11-04 Basf Ag Dry microorganism cultures and methods for their production
KR101088073B1 (en) 2010-10-16 2011-12-01 주식회사 샤인 Battery having electrode structure with metal long fibers and method of fabricating the same
DK2654417T3 (en) 2010-12-23 2018-10-29 Dupont Nutrition Biosci Aps COLD PROTECTIVE COMPOSITIONS AND APPLICATIONS THEREOF
CN102408993B (en) * 2011-11-23 2013-06-19 陕西农产品加工技术研究院 Bifidobacterium bifidum anti-freeze culture medium and application method thereof

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FR3614M (en) * 1965-07-01 1965-10-18 Carlo Giuseppe Sigurta Anhydrous, stable compositions of lactobacilli, yeast-streptococci and some other species of bacilli and their preparation process.
AT275040B (en) * 1967-04-11 1969-10-10 Werner Buehrdel Process to extend the viability and to facilitate the therapeutic applicability of freeze-dried bacterial cultures
AU1087176A (en) * 1975-03-03 1977-08-11 Miles Lab Water soluble microbial composition
EP0265253A3 (en) * 1986-10-24 1990-01-10 Kingston Technologies, Inc. Stabilized dispersed enzyme
GB8713601D0 (en) * 1987-06-10 1987-07-15 Unilever Plc Fermentation
EP0346545B1 (en) * 1988-06-17 1995-09-13 Cominco Fertilizers Ltd. Maintenance of the viability of microorganisms for use in microbial inoculants
GB8903593D0 (en) * 1989-02-16 1989-04-05 Pafra Ltd Storage of materials
GB9002003D0 (en) * 1990-01-29 1990-03-28 Ici Plc Stabilized cultures of microorganisms
FR2676751B1 (en) * 1991-05-24 1993-09-17 Lacto Labo Sa COMPOSITION SUITABLE FOR THE CONSERVATION OF ACTIVE FUNGAL SPORES.
AU659645B2 (en) * 1991-06-26 1995-05-25 Inhale Therapeutic Systems Storage of materials

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AU684072B2 (en) 1997-12-04
SK134695A3 (en) 1996-06-05
AU6510494A (en) 1994-11-21
CA2161220A1 (en) 1994-11-10
GB9406552D0 (en) 1994-05-25
GB9308734D0 (en) 1993-06-09
PL311297A1 (en) 1996-02-05
CZ280595A3 (en) 1996-02-14
BR9406488A (en) 1996-01-09
CN1121731A (en) 1996-05-01
HU9503064D0 (en) 1995-12-28
JPH08509374A (en) 1996-10-08
HUT72846A (en) 1996-05-28
NZ263867A (en) 1997-10-24
EP0696316A1 (en) 1996-02-14
WO1994025564A1 (en) 1994-11-10
BG100105A (en) 1996-12-31

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