KR960701218A - 진단법에서 유용한 재조합 엡스타인-바르 바이러스의 캡시드 항원(recomb inant epstein-barr viral capsid antigen useful in diagnostic method) - Google Patents

진단법에서 유용한 재조합 엡스타인-바르 바이러스의 캡시드 항원(recomb inant epstein-barr viral capsid antigen useful in diagnostic method) Download PDF

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KR960701218A
KR960701218A KR1019950703301A KR19950703301A KR960701218A KR 960701218 A KR960701218 A KR 960701218A KR 1019950703301 A KR1019950703301 A KR 1019950703301A KR 19950703301 A KR19950703301 A KR 19950703301A KR 960701218 A KR960701218 A KR 960701218A
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ebv
protein
recombinant
epstein
patient
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케이. 프라카시 라메시
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찰스 디. 에버트
데라테크 인코오퍼레이티드
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Publication of KR960701218A publication Critical patent/KR960701218A/ko

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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
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    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
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    • C07K4/00—Peptides having up to 20 amino acids in an undefined or only partially defined sequence; Derivatives thereof
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    • C12N2710/00011—Details
    • C12N2710/14011—Baculoviridae
    • C12N2710/14111—Nucleopolyhedrovirus, e.g. autographa californica nucleopolyhedrovirus
    • C12N2710/14141—Use of virus, viral particle or viral elements as a vector
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    • C12N2710/00011—Details
    • C12N2710/16011—Herpesviridae
    • C12N2710/16211—Lymphocryptovirus, e.g. human herpesvirus 4, Epstein-Barr Virus
    • C12N2710/16222—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes

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Abstract

재조합 EBV-gp 125 단백질은 EBV VCA-IgG 및 VCA-IgM 항체의 정량 검출을 위한 elisa와 같은 분석에서 이것의 사용을 가능하게 하는 충분한 양과 순도로 baculovirus 발현 시스템에서 만들어진다. 재조합 gp 125 단백질은 EBV-감염 사람 세포 라인으로부터 분리된 EBV-gp 125 바이러스의 캡시드 항원의 크기와 유사하고 VCA-Ig 항체에 대한 면역학적 분석에서 유사한 민감성 및 특이성을 갖는다.

Description

진단법에서 유용한 재조합 엡스타인-바르 바이러스의 캡시드 항원(RECOMBINANT EPSTEIN-BARR VIRAL CAPSID ANTIGEN USEFUL IN DIAGNOSTIC METHOD)
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음

Claims (8)

  1. EBV VCA 항체에 대한 면역분석에서 도움이 되는 충분한 선택성과 민감성을 갖는 정제된 재조합 EBV-gp 125 단백질.
  2. baculovirus 발현 시스템을 이용하여 만들어진 정제된 재조합 EBV-gp 125 단백질.
  3. baculovirus 발현 시스템을 재조합 EBB-gp 125 단백질을 제조 및 결과 산물의 친화성 크로마토그래피에 의한 정제 단계로 이루어진 정제된 EBV-gp 125 단백질의 제조방법.
  4. 재조합 EBV-gp 단백질-결합 항체가 환자의 혈청 샘플에 존재하는지를 결정하는 것으로 이루어진, 환자에서 현재 또는 과거의 EBV 감염의 진단방법.
  5. 제4항에 있어서, 상기의 결정 단계가 효소-결합 분석으로 이루어진 것을 특징으로 하는 방법.
  6. 제5항에 있어서, 상기의 효소-결합 분석이 ELISA로 이루어진 것을 특징으로 하는 방법.
  7. 제4항에 있어서, 상기 결정 단계가 면역형광 분석으로 이루어진 것을 특징으로 하는 방법.
  8. 지지대에 부착된 재조합 EBV-gp 125 단백질로 이루어진, 환자에서 현재 또는 과거 EBV 감염의 혈청학적 진단을 수행하기 위한 장치.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019950703301A 1993-02-23 1994-02-23 진단법에서 유용한 재조합 엡스타인-바르 바이러스의 캡시드 항원(recomb inant epstein-barr viral capsid antigen useful in diagnostic method) KR960701218A (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US2195193A 1993-02-23 1993-02-23
US08/021,951 1993-02-23
PCT/US1994/001775 WO1994019495A1 (en) 1993-02-23 1994-02-23 Recombinant epstein-barr viral capsid antigen useful in diagnostic method

Publications (1)

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KR960701218A true KR960701218A (ko) 1996-02-24

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KR1019950703301A KR960701218A (ko) 1993-02-23 1994-02-23 진단법에서 유용한 재조합 엡스타인-바르 바이러스의 캡시드 항원(recomb inant epstein-barr viral capsid antigen useful in diagnostic method)

Country Status (6)

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EP (1) EP0687308A4 (ko)
JP (1) JPH08507681A (ko)
KR (1) KR960701218A (ko)
AU (1) AU674936B2 (ko)
CA (1) CA2150691A1 (ko)
WO (1) WO1994019495A1 (ko)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011127299A1 (en) * 2010-04-09 2011-10-13 The General Hospital Corporation Methods for the treatment of autoimmune diseases

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3706233A1 (de) * 1987-02-26 1988-09-08 Behringwerke Ag Immunogene fusionsproteine, die epstein-barr-virusproteine enthalten

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EP0687308A1 (en) 1995-12-20
AU6269394A (en) 1994-09-14
WO1994019495A1 (en) 1994-09-01
JPH08507681A (ja) 1996-08-20
EP0687308A4 (en) 1997-07-09
CA2150691A1 (en) 1994-09-01
AU674936B2 (en) 1997-01-16

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