KR960037831A - Plasmid vector for high expression of human lipocotin-I protein from yeast - Google Patents

Plasmid vector for high expression of human lipocotin-I protein from yeast Download PDF

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KR960037831A
KR960037831A KR1019950007826A KR19950007826A KR960037831A KR 960037831 A KR960037831 A KR 960037831A KR 1019950007826 A KR1019950007826 A KR 1019950007826A KR 19950007826 A KR19950007826 A KR 19950007826A KR 960037831 A KR960037831 A KR 960037831A
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South Korea
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lipocotin
protein
yeast
plasmid vector
plasmid
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KR1019950007826A
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Korean (ko)
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KR0145947B1 (en
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정봉현
남수완
김병문
박영훈
이상기
장순재
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김은영
한국과학기술연구원
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/10—Plasmid DNA
    • C12N2800/102—Plasmid DNA for yeast

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
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  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
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Abstract

본 발명은 효모로부터 인간 리포코틴-I 단백질을 효율적으로 발현 분비시키는 플라스미드 벡터, 상기 플라스미드 벡터로 형질전환된 재조합 효모, 및 상기 효모의 배양을 통한 인간 리포코틴-I 단백질의 생산방법에 관한 것으로, 본 발명의 발현 벡터는 GAL10프로모터, 이누리나제 분비시그날, 리포코틴-I 구조 유전자 및 리포코틴-I 터미네이터를 포함하는 것을 특징으로 한다.The present invention relates to a plasmid vector for efficiently expressing and secreting human lipocotin-I protein from yeast, a recombinant yeast transformed with the plasmid vector, and a method for producing human lipocotin-I protein by culturing the yeast. The expression vector of the present invention is characterized by comprising a GAL10 promoter, an inulinase secretion signal, a lipocotin-I structural gene, and a lipocotin-I terminator.

Description

효모로부터 인간 리포코틴-I 단백질을 고발현 분비시키기 위한 플라스미드 벡터Plasmid vector for high expression of human lipocotin-I protein from yeast

본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음As this is a public information case, the full text was not included.

제1도는 본 발명에 따른 플라스미드 pGILT10의 제작과정을 나타낸 것이고, 제2도는 본 발명의 실시예 3에 따라 배양된 재조합 효모의 균체 단백질 및 배양 배지 단백질을 이용한 SDS-폴리아크릴아미드 겔 전기영동(SDS-PAGE)의 결과를 나타낸 것이다.Figure 1 shows the manufacturing process of the plasmid pGILT10 according to the present invention, Figure 2 shows the SDS-polyacrylamide gel electrophoresis (SDS) using the culture protein and the culture protein of recombinant yeast cultured according to Example 3 of the present invention -PAGE) results.

Claims (5)

GAL10 프로모터, 이누리나제 분비시그날, 리포코틴-I 구조 유전자 및 리포코틴-I 터미네이터를 포함하는 것을 특징으로 하는, 인간 리포코틴-I 단백질을 발현 분비시키기 위한 효모용 발현 분비 벡터.An expression secretion vector for yeast for expressing and secreting a human lipocotin-I protein, comprising a GAL10 promoter, an inulinase secretion signal, a lipocotin-I structural gene, and a lipocotin-I terminator. 제1항에 있어서, 플라스미드 pGILT10(KCTC 0142BP).The plasmid pGILT10 (KCTC 0142BP) of claim 1. 제1항에 또는 제2항의 플라스미드로 형질전환된 효모균주.A yeast strain transformed with the plasmid of claim 1. 제3항에 있어서, 플라스미드 pGILT10으로 형질전환된 사카로마이세스 세레비지애 SEY2102(KCTC 0142BP).The Saccharomyces cerevisiae SEY2102 (KCTC 0142BP) of claim 3 transformed with plasmid pGILT10. 제3항 또는 제4항의 재조합 효모 세포를 배양 배지 상에서 배양한 후, 상기 배양 배지로부터 리포코틴-I 단백질을 회수함을 포함하는, 인간 리포코틴-I 단백질의 생산 방법.A method for producing human lipocotin-I protein, comprising culturing the recombinant yeast cell of claim 3 or 4 on a culture medium, and then recovering lipocotin-I protein from the culture medium. ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.※ Note: The disclosure is based on the initial application.
KR1019950007826A 1995-04-04 1995-04-04 Plasmid vector for high expression and secretion of human lipocortin-i protein from yeast KR0145947B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
KR1019950007826A KR0145947B1 (en) 1995-04-04 1995-04-04 Plasmid vector for high expression and secretion of human lipocortin-i protein from yeast

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR1019950007826A KR0145947B1 (en) 1995-04-04 1995-04-04 Plasmid vector for high expression and secretion of human lipocortin-i protein from yeast

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KR960037831A true KR960037831A (en) 1996-11-19
KR0145947B1 KR0145947B1 (en) 1998-08-01

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