KR960014343A - Purification Method of Active Phosphorus Granulocyte Colony Stimulating Factor - Google Patents

Purification Method of Active Phosphorus Granulocyte Colony Stimulating Factor Download PDF

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Publication number
KR960014343A
KR960014343A KR1019940025767A KR19940025767A KR960014343A KR 960014343 A KR960014343 A KR 960014343A KR 1019940025767 A KR1019940025767 A KR 1019940025767A KR 19940025767 A KR19940025767 A KR 19940025767A KR 960014343 A KR960014343 A KR 960014343A
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South Korea
Prior art keywords
granulocyte colony
solution
stimulating factor
centrifugation
purification
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KR1019940025767A
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Korean (ko)
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KR100212071B1 (en
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강수형
박충일
박장현
나규홈
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유충식
동아제약 주식회사
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Publication of KR100212071B1 publication Critical patent/KR100212071B1/en

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/53—Colony-stimulating factor [CSF]
    • C07K14/535—Granulocyte CSF; Granulocyte-macrophage CSF
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/18—Ion-exchange chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/30—Extraction; Separation; Purification by precipitation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Toxicology (AREA)
  • Zoology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

숙주에서 발현된 인 과립구 콜로니 자극인자를 용해하여 활성화시키고; 활성화된 상기한 용액을 산 침전법으로 처리한 후 원심분리하고; 상기한 원심분리후의 상등액을 정제하는; 공정을 포함하는 활성형 인 과립구 콜로니 정제 방법을 사용하면 공정을 단순화하고 공정 효율을 높일 수 있으며, 아울러 공정 비용을 크게 절감할 수 있다.Dissolving and activating phosphorus granulocyte colony stimulator expressed in the host; The above activated solution was subjected to acid precipitation followed by centrifugation; Purifying the supernatant after centrifugation; The use of active granulocyte colony purification methods comprising the process can simplify the process and increase the process efficiency, while also significantly reducing the process cost.

Description

활성형 인 과립구 콜로니 자극인자의 정제방법Purification Method of Active Phosphorus Granulocyte Colony Stimulating Factor

본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음As this is a public information case, the full text was not included.

Claims (9)

숙주에서 발현된 인 과립구 콜로니 자극인자 용액을 활성화시키고; 활성화된 상기한 용액을 산 침전법으로 처리한 후 원심분리하고; 상기한 원심분리후의 상등액을 정제하는; 공정을 포함하는 활성형 인 과립구 콜로니 정제방법.Activating the phosphorus granulocyte colony stimulator solution expressed in the host; The above activated solution was subjected to acid precipitation followed by centrifugation; Purifying the supernatant after centrifugation; Active phosphorus granulocyte colony purification method comprising a step. 제1항에 있어서, 상기한 상등액의 정제는 상기한 원심분리후의 상등액의 이온교환크로마토그래피로 1차 정제하고; 상기한 1차 정제된 용액을 유안 침전법으로 처리하여 침전액을 제조하고; 상기한 침전액을 현탁시킨후 투석하여 농축액을 제조하고; 상기한 농축액을 겔 여과 크로마토그래피 2차 정제하는; 공정을 포함하는 활성형 인 과립구 콜로니 자극인자의 정제방법.The method of claim 1, wherein the purification of the supernatant is first purified by ion exchange chromatography of the supernatant after centrifugation; Treating the primary purified solution by yuan precipitation to prepare a precipitate; Suspending the precipitate and dialysis to prepare a concentrate; Gel filtration chromatography second purification of the concentrate; Purification method of the active phosphorus granulocyte colony stimulating factor comprising a step. 제1항에 있어서, 상기한 산 침전법은 상기한 인 과립형 콜로니 자극이자의 용액의 pH를 4.0∼7.0의 범위로 조절하여 침전을 형성하게 하는 것인 방법.The method according to claim 1, wherein the acid precipitation method adjusts the pH of the solution of the phosphorus granular colony stimulus to a range of 4.0 to 7.0 to form a precipitate. 제2항에 있어서, 상기 이온교환크로마토그래피는 SP-세파로스 패스트 플로우 수지를 사용하는 것인 방법.The method of claim 2, wherein the ion exchange chromatography uses SP-sepharose fast flow resin. 제4항에 있어서, 상기 SP-세파로스 패스트 플로우 수지는 40mM 인살일나트륨(pH 4.8)의 완층용액으로 평형화된 것인 방법.The method of claim 4, wherein the SP-Sepharose fast flow resin is equilibrated with a complete solution of 40 mM sodium phosphalyl sodium (pH 4.8). 제2항에 있어서, 상기 이온교환크로마토그래피는 인 과립구 자극인자를 0에서 0.3몰까지의 선형 농도 구배의 염화나트륨을 사용하여 용출하는 방법.The method of claim 2, wherein the ion exchange chromatography elutes the phosphorus granulocyte stimulating factor using a sodium chloride in a linear concentration gradient of 0 to 0.3 mol. 제2항에 있어서, 상기한 유안 침전법은 황 산암모늄 염을 30%되게 사용하게 인 과립구 콜로니 자극인자를 침전으로 회수하는 것인 방법.3. The method according to claim 2, wherein the yuan precipitation method recovers the phosphorus granulocyte colony stimulating factor by precipitation using 30% of ammonium sulfate salt. 제2항에 있어서, 상기한 현탁은 0.004% 폴리소르베이트 80이 첨가된 10mM 초산나트륨 용액(pH 4.0)을 사용하여 행하여지는 것인 방법.The method of claim 2, wherein the suspension is performed using 10 mM sodium acetate solution (pH 4.0) with 0.004% polysorbate 80 added. 제2항에 있어서, 상기한 겔 여과 크로마토그래피는 세파덱스 G-75 슈퍼파인 수지를 사용하는 것인 방법.The method of claim 2, wherein said gel filtration chromatography uses Sephadex G-75 superfine resin. ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.※ Note: The disclosure is based on the initial application.
KR1019940025767A 1994-10-08 1994-10-08 Method of purification of active human granulocyte colony stimulating factor KR100212071B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
KR1019940025767A KR100212071B1 (en) 1994-10-08 1994-10-08 Method of purification of active human granulocyte colony stimulating factor

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Application Number Priority Date Filing Date Title
KR1019940025767A KR100212071B1 (en) 1994-10-08 1994-10-08 Method of purification of active human granulocyte colony stimulating factor

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KR960014343A true KR960014343A (en) 1996-05-22
KR100212071B1 KR100212071B1 (en) 1999-08-02

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20040025102A (en) * 2002-09-18 2004-03-24 (주)삼세대시공 Light weight aerated concrete construction automatic system
KR102597898B1 (en) * 2023-06-14 2023-11-02 강창호 Concrete mixer

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100374306B1 (en) * 1995-07-14 2003-07-12 주식회사 엘지생명과학 Purification method of g-csf from recombinant yeast
DE102005033250A1 (en) 2005-07-15 2007-01-18 Bioceuticals Arzneimittel Ag Process for purifying G-CSF
KR20070091081A (en) * 2007-07-27 2007-09-07 주식회사 핸슨바이오텍 Method for purifying human granulocyte microphage colony stimulating factor from the suspension culture broth of oryza sativa

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20040025102A (en) * 2002-09-18 2004-03-24 (주)삼세대시공 Light weight aerated concrete construction automatic system
KR102597898B1 (en) * 2023-06-14 2023-11-02 강창호 Concrete mixer

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