KR960004497B1 - Liquid detergent composition containing enzyme - Google Patents

Abstract

This is new cleaner composition to which enzyme is added and has good skin stability and feeling. The detergent, which is composed of multivalent alcohol 10-20wt.%, inorganic salt 5-15wt.% and chinese medicine element 5-20wt.%, contains 0.1-10wt.% of granite phophyry immobilized enzyme. And it contains 1-10wt.% of C8-C14 alkyl polyglucoside or potassium salt of fatty acid additionally. The above granite phophyry immobilized enzyme is such as alkali protease, papain and lipase adsorbed on granite phophyry.

Description

Enzyme Added Liquid Detergent Composition

The present invention relates to an enzyme-added cleaning composition having excellent skin stability and usability and excellent long-term stability of the enzyme. More specifically, the present invention relates to a liquid detergent composition containing inorganic salts such as anhydrous sodium sulfate, sodium hydrogen carbonate, and polyhydric alcoholol of an adsorbed and immobilized enzyme on elvan rock powder.

In general, contamination by fats or minerals is easily removed by detergents such as soaps and detergents, but contamination by proteins in the body and coating is not easily removed. Therefore, enzymes may be added to soaps or detergents to provide a cleaning effect. How to increase has been studied. Although enzymes are stable in acidity and easily lose their activity in alkalis, their use in soap and liquid detergents has been limited, but several methods using enzymes stable in alkalis have been studied and put into practice.

For example, Novo's European Patents EP 0214761 and EP 0258068 describe detailed descriptions of lipases extracted from microorganisms and examples added to detergents, and Unilever's European Patent EP 0407225 describes genes. Variation describes the preparation for enzymes that are stable in alkali. However, enzymes are prone to loss of enzymes and prolonged side effects such as skin irritation during long-term storage, and gene mutations are also cumbersome for practical use.

In addition, enzymes tend to lose their activity in aqueous solutions more easily than they exist in powder or solid state, which is known to cause water molecules to lose activity by hydrolyzing the peptide bonds of enzymes. do.

In order to increase the stability of enzymes in aqueous solution, polyhydric alcohols such as polyethylene glycol, propylene glycol, glycerin and sorbitol are added to block water molecules that attack peptide bonds of enzymes. have.

Accordingly, the present inventors have focused on the fact that elvan is generally a porous substance, and has an absorbing effect of waste products, so that it is effective in skin softening effect and anti-aging when used in washing or bathing. After the adsorption fixation and the polyhydric alcohol and the inorganic salt were mixed, it was found that the stability of the enzyme was improved and the skin irritation was significantly reduced, thereby completing the present invention.

The present invention provides a detergent composition comprising 0.1 to 10 parts by weight of ganbanite immobilizing enzyme, 10 to 20 parts by weight of polyhydric alcohol, and 5 to 15 parts by weight of inorganic salts, adsorbing 0.1 to 10 parts by weight of alkaline protease and papain on gannet. do.

The present invention is also characterized in that it contains 0.1 to 10 parts by weight of elvan immobilized enzyme, 10 to 20 parts by weight of polyhydric alcohol, and 5 to 15 parts by weight of inorganic salts, which are adsorbed on 0.1 to 10 parts by weight of alkali protease, papain and rapase. It provides a detergent composition.

In the cleaning composition according to the present invention, the polyhydric alcohol is preferably propylene glycol, polyethylene glycol, sorbitol or glycerin, and the inorganic salt is preferably anhydrous sodium sulfate, sodium carbonate or sodium hydrogencarbonate.

The enzyme used in the cleaning composition of the present invention is preferably a protease and papain, which are proteolytic enzymes, and 50% of a lipase, which is a lipolytic enzyme, is preferably used in the cleaning agent without a surfactant. Protease is an animal-based proteolytic enzyme, which shows the highest activity in acid but high activity in neutral and alkali when alkaline protease is used. Papain is a plant-based proteolytic enzyme that is known to have high activity in acid, neutral and alkali. The method of adsorbing enzyme to elvan is performed by adding 25 g of elvan and 0.1-10 parts by weight of protein and lipolytic enzyme to 500 ml of pure water, and then stirring it for 30 minutes at 4 ° C. to filter out the residue by filtering paper with enzyme sufficiently adsorbed on elvan. Method can be used.

The cleaning composition according to the present invention has been found to greatly improve the feeling of use when it further contains one or two or more herbal ingredients selected from licorice, Angelica and ginkgo biloba extracts, which are effective for fatigue recovery by promoting skin protection and blood circulation. .

In the detergent composition according to the present invention, when the alkyl poly glucoside (APG), which is a nonionic surfactant having several hydroxyl groups, was added, the detergent composition and the skin irritation were not significantly affected, and the cleaning power was also improved. The alkylpolyglucoside (APG) used to improve the detergency is preferably a glucose unit having 2.3 or less carbon atoms from 8 to 14, as shown in the following general formula.

More specifically, the cleaning composition of the present invention is used alone or mixed 5 to 20 parts by weight of licorice, Angelica, ginkgo extract having skin softening effect and blood circulation promoting effect to 40 to 60 parts by weight of ionized water, propylene glycol, polyethylene glycol, sorbitol , 10 to 20 parts by weight of polyhydric alcohols such as glycerin, 5 to 15 parts by weight of inorganic salts such as anhydrous sodium sulfate, sodium carbonate and sodium hydrogen carbonate, 0.1 to 10 parts by weight of ganbanite immobilizing enzyme and alkyl polyglucoside (APG) or fatty acid A face wash and bath liquid cleaning agent containing 1 to 10 parts by weight of potassium.

When using less than 0.1 parts by weight of elvan immobilized enzyme is difficult to feel the cleaning effect, when used more than 10 parts by weight may cause a decrease in the feeling of use and skin irritation. In addition, when using less than 5 parts by weight of herbal components and inorganic salts, the skin softening effect is reduced, the herbal components have a disadvantage in that the feeling of use decreases when using more than 20 parts by weight and the inorganic salts are precipitated as crystals when used in more than 15 parts by weight.

The present invention will be described in more detail with reference to the following examples.

EXAMPLE

Next, a detergent composition having the composition shown in Table 1 was prepared.

The fatty acid potassium used in Table 1 used potassium lauryl acid and potassium myristic acid, and the composition ratio of the enzyme is shown in Table 2 below.

Skin stability and long-term storage stability of the cleaning composition of Examples 1 to 3 and Comparative Examples 1 to 3 were tested as follows.

(1) skin stability test

In order to measure the skin stability of the cleaning agents of the Examples and Comparative Examples after performing a patch test with 8% solution for 24 hours on the arms of 20 adult men and women, the result of visually determining the degree of stimulation is shown in Table 3 below. .

The figures represent the number of people and the classes are as follows:

-: Normal ±: Mild erythema

+: Erythema ++: erythema and edema

From the above Table 3, it can be seen that the stimulation is actually reduced by the adsorption and immobilization of the enzyme to the ganban rock more than +, the stimulation of the alkyl polyglucoside is mild compared to the anionic surfactant (potassium soap). .

(2) Long-term storage stability test

In order to test the stability during long-term storage, the protein resolution was tested as follows to determine the residual activity of the enzyme after storing for 2 weeks in a 50 ℃ thermostat. First, 50 ml of a 1% sodium sulfite (Na ₂ SO ₃ ) solution was filled in a test tube in a 50 ° C. thermostat, and 1 ml of a detergent was added thereto, followed by stirring for about 2 minutes. After adding 2 ml of 0.4% NN dimethyl casein (DMC) and stirring, the low-molecular peptide bond of dimethyl casein is decomposed by the enzyme to free the free amino group. When 0.5 ml of 0.1% 2,4,6-trinitrobenzenesulfonic acid (TNBS) is added thereto, it reacts with free amino groups to become red and gradually turn yellow. The test tube was cooled to 25 ° C., and then the absorbance at 425 nm was measured to compare enzyme activity.

Standard 1 is Example 1, and Standard 2 is Comparative Example 1 prepared before the experiment. The numerical value is calculated based on the absorbance of the standard product based on 100, and it can be seen that the stability is increased by adsorbing and immobilizing the enzyme on the elvan, and the stability is better when the alkyl polyglucoside is used than the anion.

Claims (9)

A detergent composition comprising a polyhydric alcohol, an inorganic salt, and an herbal ingredient, wherein the detergent composition contains 0.1 to 10% by weight of elvan rock-immobilized enzyme per total weight of the composition. The detergent composition according to claim 1, wherein the polyhydric alcohol is propylene glycol, polyethylene glycol, sorbitol or glycerin, and the content thereof is 10 to 20% by weight based on the total weight of the composition. The composition of claim 1 wherein the inorganic salt is anhydrous sodium sulfate, sodium carbonate or sodium hydrogen carbonate, the content of which is 5 to 15 weight percent per total weight of the composition. The detergent composition according to claim 1, wherein the herbal component is selected from licorice, Angelica and ginkgo extract. The cleaning composition according to claim 4, wherein the herbal component is present in an amount of 5 to 20% by weight based on the total weight of the composition. The detergent composition of claim 1, further comprising C ₈ -C ₁₄ alkylpolyglucoside or fatty acid potassium. 7. A detergent composition according to claim 6, which contains 1 to 10% by weight of alkylpolyglucoside or fatty acid potassium per total weight of the composition. The cleaning composition according to claim 1, wherein the elvan immobilizing enzyme adsorbs alkali protease, papain and lipase to the elvan. The cleaning composition according to claim 1, wherein the elvan immobilizing enzyme is an adsorbed alkylliprotease and papain to the elvan.