KR960001814B1 - Method for producing betalaine - Google Patents

Method for producing betalaine Download PDF

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Publication number
KR960001814B1
KR960001814B1 KR92004558A KR920004558A KR960001814B1 KR 960001814 B1 KR960001814 B1 KR 960001814B1 KR 92004558 A KR92004558 A KR 92004558A KR 920004558 A KR920004558 A KR 920004558A KR 960001814 B1 KR960001814 B1 KR 960001814B1
Authority
KR
South Korea
Prior art keywords
ppm
per liter
betalaine
microgram per
naa
Prior art date
Application number
KR92004558A
Other languages
Korean (ko)
Other versions
KR930019821A (en
Inventor
Young-Dong Kang
Hoon-Ill Oh
Original Assignee
Crown Confectionery Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Crown Confectionery Co Ltd filed Critical Crown Confectionery Co Ltd
Priority to KR92004558A priority Critical patent/KR960001814B1/en
Publication of KR930019821A publication Critical patent/KR930019821A/en
Application granted granted Critical
Publication of KR960001814B1 publication Critical patent/KR960001814B1/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • C12N5/12—Fused cells, e.g. hybridomas
    • C12N5/14—Plant cells

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Botany (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

The preparation method for betalaine (I) from cell culture of sugarbeet (II) callus consist of (1) enriching (II) at 14-18 hours at light and 6-9 hours at dark and 23-27 deg. C for 25-30 days on the medium containing 1-naphthalene acetic acid (NAA) 1-10 ppm, 6-benzylaminopurine (6-BA) 0.01-2.0 ppm, casamino acid 300-800 microgram per liter and adenine sulfate 50-300 microgram per liter, and (2) transfering (II) into the medium of tyrosine 10-30 microgram per liter, NAA 0.01-1.0 ppm, 6-BA 0.01-1.0 ppm, sucrose 60-150 gram per liter and culturing for 6-10 days at the same temperature to produce (I). At (2) condition, the concentration of auxin must be more than cytokinin.
KR92004558A 1992-03-19 1992-03-19 Method for producing betalaine KR960001814B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
KR92004558A KR960001814B1 (en) 1992-03-19 1992-03-19 Method for producing betalaine

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR92004558A KR960001814B1 (en) 1992-03-19 1992-03-19 Method for producing betalaine

Publications (2)

Publication Number Publication Date
KR930019821A KR930019821A (en) 1993-10-19
KR960001814B1 true KR960001814B1 (en) 1996-02-05

Family

ID=19330622

Family Applications (1)

Application Number Title Priority Date Filing Date
KR92004558A KR960001814B1 (en) 1992-03-19 1992-03-19 Method for producing betalaine

Country Status (1)

Country Link
KR (1) KR960001814B1 (en)

Also Published As

Publication number Publication date
KR930019821A (en) 1993-10-19

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