KR960000242A - Radix extract inhibiting the production of interleukin-6 and its preparation method - Google Patents

Abstract

The present invention relates to various extracts isolated from the roots of Stephania tetrandra S. Moore, methods for their preparation, and pharmaceutical compositions comprising the same, which are important factors for regulating the immune system of the human body using them. By regulating the production of interleukin-6 (IL-6), it effectively regulates collagen production, reactive oxygen production, and cell growth of synoviocytes in patients with arthritis. Can cure.

Description

Radix extract inhibiting the production of interleukin-6 and its preparation method

Since this is an open matter, no full text was included.

Figure 1 shows the cytotoxicity assay results of the extracts A and B

Figure 2 shows the results of measuring the inhibitory effect of interleukin-6 production of human monocytes / macrophages by the secretory extract A and B,

Figure 3 shows the results of measuring the inhibitory effect of interleukin-6 production of rat lung macrophages by the secretion extract A.

Claims (15)

Stephania tetrandra S. Moore root extract, useful for the treatment of immune diseases caused by interleukin-6 overproduction by inhibiting the production of interleukin-6. The method of claim 1, further comprising extracts with methanol; Extract with ethanol, extract with CH ₂ CI ₂ ; Or Bungigi root extract, which is an extract by an aqueous solution. The method according to claim 2, wherein 1 to 3 liters of methanol per kilogram of dried spinneret root is added to extract the spinneret root extract prepared by the method comprising extracting at 50 to 70 ℃ for at least 4 hours. The method of claim 2, wherein the root extract according to claim 3 is fractionated into 200 to 400 ml of methanol and 200 to 400 ml of n-hexane per 100 g to obtain a methanol layer, and then concentrated under reduced pressure to remove methanol, ammonium hydroxide or Adjusting the pH to 9-11 with sodium hydroxide and then fractionating 400-800 mL of distilled water: CH ₂ CI ₂ (1: 1 (v / v)) to separate the CH ₂ CI ₂ layer. Radix root extract prepared by. The method of claim 2, wherein the dried spinneret roots are crushed and filtered, and then suspended in an aqueous solution such as sterilized distilled water or physiological saline to a concentration of 50 to 200 mg / ml, followed by 4 to 12 hours at 80 to 100 ° C. Bunbang root extract prepared by the method comprising the step of boiling water. The method according to claim 2, wherein 1 to 3 liters of distilled water is added per 1 kg of dried spinner roots, heated to 80 to 100 ° C. for 4 to 12 hours, filtered and concentrated under reduced pressure, followed by 2 at -70 to -90 ° C. Spinach root extract prepared by the method comprising the step of leaving for 10 to 10 hours and lyophilized for 4 to 8 hours. The method according to claim 2, wherein 1 to 3 L of ethanol per 1 kg of dried powdered roots is added to extract the powdered roots prepared by the method comprising extracting at 60 to 90 ° C. for at least 4 hours. The branched root extract of claim 1, useful for the treatment of diseases associated with collagen synthesis, reactive oxygen production, proliferation of arthritis synovial cells, and an increase in serum GOT and GPT. The method according to claim 8, which is rheumatoid arthritis, cirrhosis, psoriasis dermatosis, multiple myeloma, cardiac myxoma, silicosis or AIDS of said disease. 1 to 3 L of methanol per 1 kg of weight of dried spinneret root, and extracting at least 50 hours at 50-70 ° C. The root extract according to claim 10 was fractionated into 200 to 400 ml of methanol and 200 to 400 ml of n-hexane per 100 g to obtain a methanol layer, and then concentrated under reduced pressure to remove methanol, and the pH was adjusted to 9 with ammonium hydroxide or sodium hydroxide. To an alkaloid fraction of the root extract extract comprising the step of adjusting to 11 to 400 to 800 mL of distilled water: CH ₂ CI ₂ (1: 1 (v / v)) to separate the CH ₂ CI ₂ layer Manufacturing method. 1 kg of dried spinneret roots, crushed and filtered, suspended in an aqueous solution to a concentration of 50 to 200mg / ml, and then sprayed for 4-12 hours at 80-100 ° C Method of Preparation of Extracts. 1 to 3 liters of distilled water per 1 kg of dried spinneret root was added, heated to 80 to 100 ° C. for 4 to 12 hours, filtered and concentrated under reduced pressure, and then left at -70 to -90 ° C. for 2 to 10 hours. Method for producing a powdered root extract comprising the step of lyophilization for 4 to 8 hours. 1 to 3 L of ethanol per 1 kg of weight of the dried spinneret root, and extracting at 60 to 90 ° C. for at least 4 hours. A pharmaceutical composition useful for the treatment of immune diseases caused by interleukin-6 overproduction, comprising an effective amount of the branched root extract of any one of claims 1 to 9. ※ Note: The disclosure is based on the initial application.