KR950701684A - 뎅그열 바이러스 혈청형 1(싱가폴균주)의 cDNA서열(cDNA sequence of dengue virus serotype 1(Singapore strain)) - Google Patents

뎅그열 바이러스 혈청형 1(싱가폴균주)의 cDNA서열(cDNA sequence of dengue virus serotype 1(Singapore strain))

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KR950701684A
KR950701684A KR1019940703865A KR19940703865A KR950701684A KR 950701684 A KR950701684 A KR 950701684A KR 1019940703865 A KR1019940703865 A KR 1019940703865A KR 19940703865 A KR19940703865 A KR 19940703865A KR 950701684 A KR950701684 A KR 950701684A
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polypeptide
ecacc
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dengue virus
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지안린 후
분환 단
인휘 단
유한 얍
워청 찬
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인휘 단
내셔날 유니버서티 오브 싱가폴
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Abstract

본 발명의 DEN1-S275/90(제 ECACC V92042111호)은 뎅그열 바이러스 혈청형 1의 신규한 균주에 관한 것이다. 이 바이러스의 완전한 cDNA서열은 클론된 바 있으며 이의 단백질-암호화 단편은 발현 플라스미드 작제시 사용된 바 있다. 불활성화된 형태의 DEN1-S275/90, DEN1-S275/90폴리펩타이드 또는 이의 융합 단백질은 DEN1-S275/90 및 기타 DEN1바이러스에 대한 면역화용 백신내에 혼입될 수 있다. 본 발명은 추가로 진단시약, 즉 DEN1-S275/90 단백질에 대한 표지된 항체, 및 DEN1 바이러스를 검출하기 위한 키트를 제공한다.

Description

뎅그열 바이러스 혈청형 1(싱가폴균주)의 cDNA서열(cDNA sequence of dengue virus serotype 1(Singapore strain))
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
제1도는 뎅그열 바이러스 제1형(싱가폴 균주 S275/90)의 cDNA 및 발현 벡터내에서 O+DNA의 단편의 개략도이고;
제2도는 프룬드 완전 보조제(CFA)가 있거나 없이 실시예 2내지 5에서 제조된 융합 단백질로 면역화시킨 후에 마우스내 혈청학적 반응을 확인하는 겔 결과를 나타낸다. 겔레인 ; 레인 1:M, 레인 2:항 E, 레인 3:항-E+CFA, 레인 4:항-NS1, 레인 5:항-NS2, 레인 6:항-NS2+CFA, 레인 7:항-NS3, 레인 8:항-NS3+CFA, 레인 9:항-NS5, 레인 10:항-NS%+CAF, 레인 11:양성 래빗 혈청 레인 12: 음성 래빗 혈청, 레인 13:M;
제3도는 실시예 2내지 5에서 제조된 융합 단백질로 면역화한 후 래빗에 있어서 혈청학적 반응을 확인하는 겔 결과를 나타낸다. (-), 면역화전 혈청: (+)면역화후 혈청, 겔 레인: 1:(-), 레인 2:(+)항-E, 레인 3:(-), 레인 4:(+)항-NS1, 레인 5:(-), 레인 6:(+)-NS2, 레인 7:(-), 레인 3:(+)항-NS3, 레인 9:(-), 레인 10:(+)항-NS5, 레인 11:양성 뎅그열:레인 12:환자혈청;
제4도는 뎅그열 제1형 DI-275를 감염시키고 제조합 융합 단백질에 대한 항체로 프로브화한 C6/36세포의 형광 현미경 검경을 도시한 것이다. A. 대조군 항혈청; B, 항-E; C, 항-NS1;D, 항-NS2;E, 항-NS3;F항-NS5.

Claims (17)

  1. DEN1-S275/90(제 ECACC V92042111호).
  2. 불활성화된 형태의 DEN-S275/90(제 ECACC V92042111호).
  3. 실질적으로 서열확인번호 1에 나타낸 DEN1-S275/90(제 ECACC V92042111호)을 암호화하는 DNA폴리노클레오타이드.
  4. DEN1-S275/90(제 ECACC V92042111호)의 C,C', FreM, M, E, NS1, NS2A, NS3, NS4A, NS4B, 또는 NS5유전자를 암호화 하는, 제3항에 따른 DNA 폴리뉴클레오타이드의 단편.
  5. 발현 벡터내의 제3항 또는 제4항에 따른 DNA폴리뉴클레오타이드 또는 이의 단편.
  6. pGEX-KG/EX-20, pMAL-c/NS1-104, pMAL-cR1/NS2-1, pGEX-KG/NS3 BB c600-1 및 pGEX-KG/NS5 c600 HF1으로부터 선택된 제5항에 따른 발현 벡터.
  7. 제5항 또는 제6항에 따른 발현 발현 벡터를 지닌 세포.
  8. 제7항에 있어서, 이, 콜라이 또는 곤충 세포인 세포.
  9. DEN1-S275/90(제 ECACC V2042111호)의 C,C', PreM, M, E, NS1, NS2A, NS2B, NS4A, NS4B, 또는 NS5 폴리펩타이드인 실질적으로 분리된 형태로서의 폴레펩타이드.
  10. 제9항에 있어서, 융합 단백질 형태의 폴리펩타이드.
  11. 제6항에 따른 발현 벡터로부터 선택된 발현 벡터에 의해 암호화된 제10항에서 청구된 융합 단백질.
  12. 제7항 또는 제8항에 따른 세포주를 배양하여 폴리펩타이드를 회수함을 포함하여 제9항 내지 제11항 중 어느 한 항에 청구된 폴리펩타이드를 제조하는 방법.
  13. 제9항에 있어서, 표지물을 갖는 폴리펩타이드.
  14. 약제학적으로 허용되는 담체 또는 희석재와 배합된 제9항 내지 제11항중 어느 한 항에 청구된 하나 이상의 폴리펩타이드 또는 제2항에 청구된 불활성화된 바이러스를 포함하는 백신.
  15. 제14항에 있어서, 하나의 폴리펩타이드가 DEN1바이러스 단백질에 대한 항체를 유도해낼 수 있는 E, NS1, NS2, NS3, NS5 및 이의 융합 단백질로부터 선택된 백신.
  16. DEN1바이러스 단백질에 결합할 수 있으며, 임의로 참조용 표지를 지니고 있는 제9항 내지 제11항중 어느 한 항에서 청구된 폴리펩타이드에 대한 항체.
  17. 고체 지지체에 고정된 제16항에 따른 항체 또는 제9항 또는 제13항에 큰 폴리펩타이드를 포함하는, DEN1바이러스의 존재 또는 부재를 검출하기 위한 시험 키트.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019940703865A 1992-04-29 1993-04-28 뎅그열 바이러스 혈청형 1(싱가폴균주)의 cDNA서열(cDNA sequence of dengue virus serotype 1(Singapore strain)) KR950701684A (ko)

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GB929209243A GB9209243D0 (en) 1992-04-29 1992-04-29 Dengue virus
GBWP9209243.6GH 1992-04-29
PCT/CA1993/000182 WO1993022440A1 (en) 1992-04-29 1993-04-28 Cdna sequence of dengue virus serotype 1 (singapore strain)

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KR950701684A true KR950701684A (ko) 1995-04-28

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CA (1) CA2134666A1 (ko)
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US6676936B1 (en) 1988-07-14 2004-01-13 The United States Of America As Represented By The Department Of Health And Human Services. Chimeric and/or growth-restricted flaviviruses
WO1996040933A1 (en) * 1995-06-07 1996-12-19 The Government Of The United States Of America, Represented By The Secretary Department Of Health And Human Services Infectious dengue 2 virus pdk-53 as quadravalent vaccine
DE19613253A1 (de) * 1996-04-02 1997-10-09 Immuno Gmbh Technik zum Nachweis von Infektionen mit dem TBE-Virus und anderen Flaviviren
FR2767324B1 (fr) * 1997-08-14 2001-04-06 Pasteur Institut Utilisation de proteines d'enveloppe recombinantes pour le diagnostic du virus de la dengue
US5968732A (en) * 1997-12-31 1999-10-19 Akzo Nobel, N.V. Isothermal transcription based assay for the detection and genotyping of dengue virus
FR2794865B1 (fr) 1999-06-09 2003-04-18 Pasteur Institut Methode de detection precoce des flavivirus et ses applications
US6855521B2 (en) * 1999-12-01 2005-02-15 The United States Of America As Represented By The Secretary Of The Navy Serotype and dengue group specific flurogenic probe based PCR (TaqMan) assays against the respective C and NS5 genomic and 3′ non-coding regions of dengue virus
AU2002211490A1 (en) * 2000-10-04 2002-04-15 The Trustees Of The University Of Pennsylvania Compositions and methods of using capsid protein from flaviviruses and pestiviruses
US20040005457A1 (en) * 2002-07-03 2004-01-08 Kimberly-Clark Worldwide, Inc. Methods of improving the softness of fibers and nonwoven webs and fibers and nonwoven webs having improved softness
ES2524048T3 (es) * 2003-03-31 2014-12-03 F. Hoffmann-La Roche Ag Composiciones y métodos para la detección de ciertos flavivirus, incluidos los miembros del serogrupo del virus de la encefalitis japonesa
EP2473624B1 (en) 2009-08-31 2019-05-01 Gen-Probe Incorporated Dengue virus assay
US10308689B2 (en) * 2010-06-24 2019-06-04 La Jolla Institute For Allergy And Immunology Dengue virus (DV) polypeptide sequences, T cell epitopes and methods and uses thereof
WO2012014116A1 (en) * 2010-07-29 2012-02-02 Bigtec Private Limited Probes and primers for detection of dengue
WO2012153153A1 (en) 2011-05-11 2012-11-15 Diagon Kft. Procedure for rapid determination of viruses using nucleic acid-based molecular diagnostics, and a kit for this purpose
BR112013030637A2 (pt) * 2011-05-26 2018-06-12 Inst De Medicina Molecular peptídeo derivado do vírus da dengue (denv) e métodos para a inibição da replicação de flavivírus

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AU4257593A (en) 1993-11-29
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EP0638122B1 (en) 1997-12-10
ATE161048T1 (de) 1997-12-15
WO1993022440A1 (en) 1993-11-11
EP0638122A1 (en) 1995-02-15
AU679179B2 (en) 1997-06-26
MY110899A (en) 1999-06-30
CA2134666A1 (en) 1993-11-11
GB9209243D0 (en) 1992-06-17
SG47384A1 (en) 1998-04-17
US6017535A (en) 2000-01-25

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