KR950026975A - Manufacturing method of high density antagonist microorganism base material - Google Patents

Manufacturing method of high density antagonist microorganism base material Download PDF

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Publication number
KR950026975A
KR950026975A KR1019940016736A KR19940016736A KR950026975A KR 950026975 A KR950026975 A KR 950026975A KR 1019940016736 A KR1019940016736 A KR 1019940016736A KR 19940016736 A KR19940016736 A KR 19940016736A KR 950026975 A KR950026975 A KR 950026975A
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base material
density
water
manufacturing
bacteria
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KR1019940016736A
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Korean (ko)
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도시유키 츠츠이
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최정희
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Virology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Botany (AREA)
  • Mycology (AREA)
  • Soil Conditioners And Soil-Stabilizing Materials (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

길항 미생물군의 고밀도 복합배양과, 미생물 대사산물을 함유하는 활성화 기재를 제공한다. 미강을 주성분으로 하는 유기질 배지에 방선균과 세균 및 사상균을 함유하는 효모를 주체로 하는 토양 미생물 혼합체를 접종하여 배양한 원균을 광물 분쇄토에 배합 영양물과 물을 배합한 영양기에 접종하여 일정시간 배양하여 이것을 과립상으로 만든다.Provided are high density complex cultures of antagonistic microbial populations and activated substrates containing microbial metabolites. Inoculate soil microorganisms mainly containing actinomycetes and bacteria and filamentous fungi into organic medium containing rice bran as a main ingredient, and inoculate nutrients mixed with nutrients and water on mineral ground to incubate for a certain period of time. This is granulated.

Description

고밀도 길항 미생물 기재의 제조방법Manufacturing method of high density antagonist microorganism base material

본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음Since this is an open matter, no full text was included.

제1도는 본 발명에 의해 제조된 고밀도 길항(拮抗) 미생물 기재(基才)를 장해토양에 시용(施用)할 경우에 있어서의 토양의 수복상태를 균의 증감치에 의해 나타낸 그래프,1 is a graph showing the state of restoration of soil in the case of applying the high-density antagonist microorganism substrate prepared according to the present invention to an impaired soil by increase or decrease of bacteria,

제2도는 본 발명에 의해 제조된 고밀도 길항 미생물 기재를 장해토양에 시용할 때에 방선균(放線菌)에 의한 사상균 (狀菌)의 제압상태 설명도.2 is a filamentous fungus caused by actinomycetes when the high-density antagonist microorganism substrate prepared according to the present invention is applied to an obstacle soil ( Explanatory drawing of suppression state of iii).

Claims (4)

미강을 주성분으로 하는 유기질 배지에 스트렙토미세스속에 속하는 방선균과 세균 및 사상균을 함유하는 효모를 주체로 하는 토양 미생물 혼합체를 접종하여 계대 배양한 원균을, 퇴적토를 함유하는 광물 분쇄토에 곡분 등의 배합 영양물과 물을 배합한 영양기에 접종하여 일정시간 배양함과 동시에 이배양기를 온도상승에 따라 적절히 교반통기하여 차츰 과립상으로 하는 것을 특징으로 하는 고밀도 길항 미생물 기재의 제조방법.Mixed nutrients, such as cereal flour, are inoculated on subcultured mineral soils by inoculating subsidiary soil microorganism mixtures containing actinomycetes belonging to the genus Streptomyces and yeasts containing bacteria and filamentous bacteria in an organic medium containing rice bran as a main ingredient. A method of producing a high-density antagonist microorganism base material, characterized by inoculating a nutrient unit containing water and water, incubating for a predetermined time, and simultaneously incubating the incubator with a temperature increase to form granules gradually. 제1항에 있어서, 유기질 배지에 의한 원균의 배양을 온도 25∼35℃, 수분함량 25∼30%의 조건하에서 1일 복수회의 교반을 행하면서 4∼5일간 행하는 것을 특징으로 하는 고밀도 길항 미생물 기재의 제조방법.The high-density antagonist microorganism substrate according to claim 1, wherein the cultivation of the progeny in the organic medium is carried out for 4 to 5 days with agitation a plurality of times a day under conditions of a temperature of 25 to 35 DEG C and a water content of 25 to 30%. Manufacturing method. 제1항에 있어서, 원균을 접종하여 배양하는 배양기가 퇴적토를 함유하는 광물 분쇄토 100중량부에 대하여 곡분 등의 배합 영양물 10∼20중량부를 첨가하고 다시 수분함량 30∼40% 정도가 되도록 물을 첨가하여 혼합함으로써 만들어지는 것을 특징으로 하는 고밀도 길항 미생물 기재의 제조방법.The method of claim 1, wherein the incubator inoculated and cultured with prokaryotic bacteria is added with 10 to 20 parts by weight of a compounded nutrient such as grain flour to 100 parts by weight of the mineral ground soil containing the sedimentary soil, and then water is added so that the water content is about 30 to 40%. It is made by adding and mixing, The manufacturing method of the high density antagonism microorganism base material characterized by the above-mentioned. 제1항에 있어서, 원균을 접종한 배양기를 차츰 과립화하면서 순차적으로 수분을 증발시키고, 또한 강제건조에 의해 수분을 최종적으로 4∼5%로 조정하는 것을 특징으로 하는 고밀도 길항 미생물 기재의 제조방법.The method for producing a high-density antagonist microorganism base material according to claim 1, wherein the incubator inoculated with progenus is gradually evaporated while gradually granulating, and the moisture is finally adjusted to 4 to 5% by forced drying. . ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.※ Note: The disclosure is based on the initial application.
KR1019940016736A 1994-03-31 1994-07-12 Manufacturing method of high density antagonist microorganism base material KR950026975A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP94-85976 1994-03-31
JP6085976A JPH07274963A (en) 1994-03-31 1994-03-31 Preparation of high-density antagonistic microorganism substrate

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100356762B1 (en) * 2000-04-10 2002-10-18 파인바이오식품 주식회사 Method to manufacture complex microbe culture and manure using complex microbe culture
KR100656980B1 (en) * 2004-08-19 2006-12-13 김만석 Method for mass-producing culture solution of micro-organisms extracted from rice bran and method for mass-producing activated solution of micro-organisms using the culture solution

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100430761B1 (en) * 2000-10-02 2004-05-10 (주)에스비바이오테크 Method for producing high-density antagonistic microbes base material
KR101436646B1 (en) 2006-06-12 2014-09-01 미츠비시 가스 가가쿠 가부시키가이샤 Method of replacing dispersion medium
US9144750B2 (en) 2006-06-12 2015-09-29 Mitsubishi Gas Chemical Company, Ltd. Method of replacing dispersion medium and apparatus therefor
JP5412831B2 (en) * 2006-07-24 2014-02-12 三菱瓦斯化学株式会社 Dispersion medium replacement method
JP5348851B2 (en) * 2007-04-09 2013-11-20 崔 貞姫 Method for producing basic cosmetic liquid phase component using microorganism

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100356762B1 (en) * 2000-04-10 2002-10-18 파인바이오식품 주식회사 Method to manufacture complex microbe culture and manure using complex microbe culture
KR100656980B1 (en) * 2004-08-19 2006-12-13 김만석 Method for mass-producing culture solution of micro-organisms extracted from rice bran and method for mass-producing activated solution of micro-organisms using the culture solution

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