KR950018470A - Method for Purifying Streptokinase - Google Patents

Method for Purifying Streptokinase Download PDF

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Publication number
KR950018470A
KR950018470A KR1019930032150A KR930032150A KR950018470A KR 950018470 A KR950018470 A KR 950018470A KR 1019930032150 A KR1019930032150 A KR 1019930032150A KR 930032150 A KR930032150 A KR 930032150A KR 950018470 A KR950018470 A KR 950018470A
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KR
South Korea
Prior art keywords
passed
cpg
streptokinase
exchange resin
purifying
Prior art date
Application number
KR1019930032150A
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Korean (ko)
Other versions
KR970002903B1 (en
Inventor
이재홍
고중환
유원일
강성구
김정현
이광섭
황수연
Original Assignee
김정순
제일제당 주식회사
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Priority to KR1019930032150A priority Critical patent/KR970002903B1/en
Publication of KR950018470A publication Critical patent/KR950018470A/en
Application granted granted Critical
Publication of KR970002903B1 publication Critical patent/KR970002903B1/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/18—Ion-exchange chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/24—Metalloendopeptidases (3.4.24)
    • C12Y304/24029—Aureolysin (3.4.24.29)

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

본 발명은 스트랩토코커스 균주를 배양 후 균체를 제거하고 농축후 양이온 교환수지에 통과시켜 불순물을 제거하고 교환수지에 통과시켜 불순물을 제거하고 통과된 액을 pH 6.5~7.0으로 조절하여 CPG컬럼에 통과시킨 다음 CPG에 흡착된 스트렙토키나제를 PH 7.0~8.0의 완충용액으로 용리하여 정제함을 특징으로 하는 스트렙토키나제의 정제방법에 관한 것이다.In the present invention, after culturing the Strain Tococcus strain, the cells are removed, concentrated and passed through a cation exchange resin to remove impurities and passed through the exchange resin to remove impurities and adjust the passed solution to pH 6.5 ~ 7.0 to pass through the CPG column The present invention relates to a method for purifying streptokinase, which is characterized by eluting and purifying streptokinase adsorbed on CPG with a buffer solution of PH 7.0-8.0.

Description

스트렙토키나제의 정제방법Method for Purifying Streptokinase

본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음Since this is an open matter, no full text was included.

Claims (2)

스트렙토코커스 균주를 배양 후 균체를 제거하고 농축후 양이온 교환수지에 통과시켜 불순물을 제거하고, 양이본 교환수지를 통과시킨 액을 pH 6.5-7.0으로 조절하여 CPG 컬림에 통과시킨 다음 CPG에 흡착된 스트렙토키나제를 pH 7.0-8.3의 완충용액으로 용리하여 정제함을 특징으로 하는 스트렙토키나제의 정제방법.After culturing Streptococcus strains, the cells were removed, concentrated and passed through a cation exchange resin to remove impurities, and the solution passed through the bimodal exchange resin was adjusted to pH 6.5-7.0 and passed through a CPG culture, and then adsorbed to CPG. A method for purifying streptokinase, characterized by eluting streptokinase with a pH 7.0-8.3 buffer. 제1항에 있어서, CPG는 포아사이즈가 120-1000Å이고, 80-400 메쉬인 것을 사용하는 정제방법.The method of claim 1, wherein the CPG has a pore size of 120-1000 mm 3 and 80-400 mesh. ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.※ Note: The disclosure is based on the initial application.
KR1019930032150A 1993-12-31 1993-12-31 Purification process of streptokinase KR970002903B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
KR1019930032150A KR970002903B1 (en) 1993-12-31 1993-12-31 Purification process of streptokinase

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR1019930032150A KR970002903B1 (en) 1993-12-31 1993-12-31 Purification process of streptokinase

Publications (2)

Publication Number Publication Date
KR950018470A true KR950018470A (en) 1995-07-22
KR970002903B1 KR970002903B1 (en) 1997-03-12

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ID=19375063

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1019930032150A KR970002903B1 (en) 1993-12-31 1993-12-31 Purification process of streptokinase

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KR (1) KR970002903B1 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20020007231A (en) * 2001-08-01 2002-01-26 이병철 The purification of streptokinase

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20020007231A (en) * 2001-08-01 2002-01-26 이병철 The purification of streptokinase

Also Published As

Publication number Publication date
KR970002903B1 (en) 1997-03-12

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