JPS57208988A - Purification of acidic carboxypeptidase - Google Patents
Purification of acidic carboxypeptidaseInfo
- Publication number
- JPS57208988A JPS57208988A JP56092661A JP9266181A JPS57208988A JP S57208988 A JPS57208988 A JP S57208988A JP 56092661 A JP56092661 A JP 56092661A JP 9266181 A JP9266181 A JP 9266181A JP S57208988 A JPS57208988 A JP S57208988A
- Authority
- JP
- Japan
- Prior art keywords
- carboxypeptidase
- sephadex
- acidic
- acidic carboxypeptidase
- carboxy
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 102000005367 Carboxypeptidases Human genes 0.000 title abstract 6
- 108010006303 Carboxypeptidases Proteins 0.000 title abstract 6
- 230000002378 acidificating effect Effects 0.000 title abstract 5
- 238000000746 purification Methods 0.000 title 1
- 229920005654 Sephadex Polymers 0.000 abstract 3
- 239000012507 Sephadex™ Substances 0.000 abstract 3
- 239000000706 filtrate Substances 0.000 abstract 2
- 241000228143 Penicillium Species 0.000 abstract 1
- 241000228147 Penicillium camemberti Species 0.000 abstract 1
- 235000002245 Penicillium camembertii Nutrition 0.000 abstract 1
- 230000001580 bacterial effect Effects 0.000 abstract 1
- 230000000694 effects Effects 0.000 abstract 1
- 244000005700 microbiome Species 0.000 abstract 1
- 239000000047 product Substances 0.000 abstract 1
- 238000001179 sorption measurement Methods 0.000 abstract 1
Landscapes
- Enzymes And Modification Thereof (AREA)
Abstract
PURPOSE: To purify acidic carboxypeptidase, in an industrial scale, by passing acidic carboxypeptidase prepared by the cultivation of microorganisms through a column packed with carboxy-sephadex.
CONSTITUTION: A bacterial strain capable of producing acidic carboxypeptidase and belonging to Penicillium genus, e.g. Penicillium caseicolum, is cultured, and the filtrate of the cultured product is passed through a column packed with carboxy-sephadex at an ionic strength of ≤0.2M and a pH of 3.0W4.0 to effect the adsorption of the acidic carboxypeptidase from the filtrate to the sephadex. The column is washed, and the adsorbed carboxypeptidase is eluted at an ionic strength of ≥0.5M and a pH of 3.0W4.0.
COPYRIGHT: (C)1982,JPO&Japio
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP56092661A JPS57208988A (en) | 1981-06-16 | 1981-06-16 | Purification of acidic carboxypeptidase |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP56092661A JPS57208988A (en) | 1981-06-16 | 1981-06-16 | Purification of acidic carboxypeptidase |
Publications (2)
Publication Number | Publication Date |
---|---|
JPS57208988A true JPS57208988A (en) | 1982-12-22 |
JPH037356B2 JPH037356B2 (en) | 1991-02-01 |
Family
ID=14060651
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP56092661A Granted JPS57208988A (en) | 1981-06-16 | 1981-06-16 | Purification of acidic carboxypeptidase |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS57208988A (en) |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2009080484A1 (en) * | 2007-12-20 | 2009-07-02 | Dsm Ip Assets B.V. | Accelerated fungal growth |
CN108275369A (en) * | 2017-12-27 | 2018-07-13 | 东莞市厚威包装科技股份有限公司 | A kind of mycelia packaging cushion of packing box |
-
1981
- 1981-06-16 JP JP56092661A patent/JPS57208988A/en active Granted
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2009080484A1 (en) * | 2007-12-20 | 2009-07-02 | Dsm Ip Assets B.V. | Accelerated fungal growth |
EP3375294A1 (en) * | 2007-12-20 | 2018-09-19 | DSM IP Assets B.V. | Accelerated fungal growth |
CN108275369A (en) * | 2017-12-27 | 2018-07-13 | 东莞市厚威包装科技股份有限公司 | A kind of mycelia packaging cushion of packing box |
Also Published As
Publication number | Publication date |
---|---|
JPH037356B2 (en) | 1991-02-01 |
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