KR950008689A - New antifungal antibiotics and new Bacillus sp. Microorganisms producing them and methods for preparing new antifungal antibiotics using the same - Google Patents

New antifungal antibiotics and new Bacillus sp. Microorganisms producing them and methods for preparing new antifungal antibiotics using the same

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Publication number
KR950008689A
KR950008689A KR1019940019211A KR19940019211A KR950008689A KR 950008689 A KR950008689 A KR 950008689A KR 1019940019211 A KR1019940019211 A KR 1019940019211A KR 19940019211 A KR19940019211 A KR 19940019211A KR 950008689 A KR950008689 A KR 950008689A
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KR
South Korea
Prior art keywords
solvent
column chromatography
producing
antifungal
bacillus
Prior art date
Application number
KR1019940019211A
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Korean (ko)
Other versions
KR970010603B1 (en
Inventor
신원철
유재홍
Original Assignee
신원철
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Publication date
Application filed by 신원철 filed Critical 신원철
Priority to KR1019940019211A priority Critical patent/KR970010603B1/en
Priority to CA002171947A priority patent/CA2171947A1/en
Priority to PCT/KR1994/000123 priority patent/WO1995007997A1/en
Priority to JP7509095A priority patent/JPH09510862A/en
Priority to GB9605096A priority patent/GB2296716A/en
Priority to AU77090/94A priority patent/AU7709094A/en
Priority to CN94193799A priority patent/CN1135238A/en
Publication of KR950008689A publication Critical patent/KR950008689A/en
Application granted granted Critical
Publication of KR970010603B1 publication Critical patent/KR970010603B1/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
    • C12P17/16—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms containing two or more hetero rings
    • C12P17/162—Heterorings having oxygen atoms as the only ring heteroatoms, e.g. Lasalocid
    • H—ELECTRICITY
    • H01—ELECTRIC ELEMENTS
    • H01S—DEVICES USING THE PROCESS OF LIGHT AMPLIFICATION BY STIMULATED EMISSION OF RADIATION [LASER] TO AMPLIFY OR GENERATE LIGHT; DEVICES USING STIMULATED EMISSION OF ELECTROMAGNETIC RADIATION IN WAVE RANGES OTHER THAN OPTICAL
    • H01S5/00—Semiconductor lasers
    • H01S5/30—Structure or shape of the active region; Materials used for the active region
    • H01S5/34—Structure or shape of the active region; Materials used for the active region comprising quantum well or superlattice structures, e.g. single quantum well [SQW] lasers, multiple quantum well [MQW] lasers or graded index separate confinement heterostructure [GRINSCH] lasers

Abstract

본 발명은 항진균성 항생물질을 생산하는 신규 바실러스 속 미생물 SY-414 및 상기 바실러스 속 미생물을 이용하여 생산된 항진균성 항생물질인 트리헥소신 및 그의 생산 방법에 관한 것이다. 식물류 진균 특히 벼의 도열병 및 문고병균에 항균능이 높은 신규 바실러스 속 미생물을 토양으로부터 분리 동정하여 YS 액체배지에서 진탕 배양하고 배양액을 원심분리하여, 배양 상등액을 에틸 아세테이트로 추출하고 용매층을 모아 용매를 제거한 후, 용매가 제거된 농축활성 부분을 모아 메탄올에 용해시킨다. 실리카 겔 컬럼 크로마토그라피를 행한 다음, 용매를 제거한 후 농축 활성부분을 디클로로메탄 : 메탄올 (1:5) 용액에 용해시켜 세파덱스 LH-20 컬럼 크로마토그라피를 행하고, 상기에서 용매를 제거한 후 활성부분을 모아 물에 용해시켜 동결건조한다. 상기에서 얻어진 분말상태의 항생물질을 메탄올에 용해시켜 냉장시켜 결정화함을 특징으로 하는 하기 일반식(I)로 표현되는 신규 항생물질 및 그의 생산 방법에 관한 것이다.The present invention relates to a novel Bacillus sp. Microorganism SY-414 that produces antifungal antibiotics, and trihexine, an antifungal antibiotic produced using the Bacillus microorganism, and a method for producing the same. Isolate and identify new Bacillus microorganisms with high antibacterial activity against soils of plant fungi, especially rice blasts and paperback bacteria from soil, shake culture in YS liquid medium, centrifuge the culture solution, extract the culture supernatant with ethyl acetate, collect solvent layer After removal, the concentrated active portion from which the solvent is removed is collected and dissolved in methanol. After silica gel column chromatography was performed, the solvent was removed, and then the concentrated active portion was dissolved in a dichloromethane: methanol (1: 5) solution to conduct Sephadex LH-20 column chromatography. Collect, dissolve in water and lyophilize. The present invention relates to a novel antibiotic represented by the following general formula (I) and a method for producing the same, characterized in that the obtained antibiotic is dissolved in methanol and refrigerated.

상기식에서, Rl, R2및 R3는 같거나 다른 것으로 -H,-OH, C1∼C6의 알킬기, C5∼C20의 알릴기 또는 C2∼C6의 알킬렌기이고, ℓ, m 및 n은 각각 1∼3의 정수이다.Wherein R 1 , R 2 and R 3 are the same or different and are -H, -OH, an alkyl group of C 1 -C 6 , an allyl group of C 5 -C 20 or an alkylene group of C 2 -C 6 , , m and n are each an integer of 1-3.

Description

신규 항진균성 항생물질과 이를 생산하는 신규 바실러스 속(Bacillus sp.) 미생물 및 이를 이용한 신규 항진균성 항생물질의 제조 방법New antifungal antibiotics and new Bacillus sp. Microorganisms producing them and methods for preparing new antifungal antibiotics using the same

본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음Since this is an open matter, no full text was included.

제1도는 바실러스 속(Bacillus sp.) SY-414 균주의 전자 현미경 사진이고,1 is an electron micrograph of the strain Bacillus sp. SY-414,

제2도는 본 발명 균주가 형성하는 포자의 전자 현미경 사진이며,2 is an electron micrograph of the spores formed by the strain of the present invention,

제3도는 배양 시간에 따른 본 발명 균주의 생육과 항생물질의 생산량을 나타낸 그래프이다.3 is a graph showing the growth and production of antibiotics of the strain of the present invention according to the culture time.

Claims (11)

하기 일반식(I)로 표현되는 신규 항진균성 항생물질.New antifungal antibiotics represented by the following general formula (I). 상기식에서, R1, R2및 R3는 같거나 다른 것으로 수소, -OH, C1∼C6의 알킬기, C5∼C20의 알릴기 또는 C2∼C6의 알킬렌기이고, ℓ, m 및 n은 각각 1∼3의 정수이다.Wherein R 1 , R 2 and R 3 are the same or different and are hydrogen, —OH, an alkyl group of C 1 to C 6 , an allyl group of C 5 to C 20 or an alkylene group of C 2 to C 6 , m and n are the integers of 1-3 respectively. 제1항에 있어서, R1, R2는 R3는 -OH이고, ℓ은 3, m은 2, n은 3인 것을 특징으로 하는 항진균성 항생물질.The antifungal antibiotic according to claim 1 , wherein R 1 and R 2 are R 3 is -OH, l is 3, m is 2, and n is 3. 항진균성 활성을 갖는 신규 바실러스 속(Bacillus sp.) SY-414 미생물(KCCM-10043).Novel Bacillus sp. SY-414 microorganism (KCCM-10043) with antifungal activity. 바실러스 속(Bacillus sp.) SY-414 균주를 액체배지에서 배양하고, 배양액을 원심분리하여, 배양상등액을 에틸 아세테이트로 추출하고, 용매층을 모아 용매를 제거한 후, 용매가 제거된 농축 활성부분을 모아 용매에 용해시켜 컬럼 크로마토그라피를 행하고, 용매를 제거한 후 농축 활성부분을 용매에 용해시켜 또 다시 컬럼 크로마토그라피를 행하고, 활성부분을 모아 용매를 제거한 후 물에 용해시켜 동결건조하고, 얻어진 분말 상태의 항생물질을 용매에 용해시켜 4℃ 냉장실에 방치시켜 결정화함을 특징으로 하는 항진균성 항생물질의 생산 방법.Bacillus sp. SY-414 strain was incubated in a liquid medium, the culture solution was centrifuged, the culture supernatant was extracted with ethyl acetate, the solvent layer was collected, the solvent was removed, and the concentrated active portion was removed. Gather and dissolve in a solvent to perform column chromatography, remove the solvent, and then concentrate the concentrated active part in a solvent and perform column chromatography again. Gather the active parts, remove the solvent, dissolve in water and freeze-dried to obtain a powder. A method for producing an antifungal antibiotic, characterized in that the antibiotic is dissolved in a solvent and left in a refrigerator at 4 ° C. for crystallization. 제4항에 있어서, 액체배지는 탄소원으로서 가용성 전분, 글로코오스, 자일로오스 또는 만니톨 중 1종 이상을 사용하는 것을 특징으로 하는 항진균성 항생물질의 생산 방법.The method for producing an antifungal antibiotic according to claim 4, wherein the liquid medium uses at least one of soluble starch, glocose, xylose or mannitol as a carbon source. 제4항에 있어서, 액체배지는 질소원으로서 육즙, 효모 추출액, 펩톤 또는 소이빈 중 1종 이상을 사용하는 것을 특징으로 하는 항진균성 항생물질의 생산 방법.The method for producing an antifungal antibiotic according to claim 4, wherein the liquid medium uses at least one of broth, yeast extract, peptone or soybean as a nitrogen source. 제4항에 있어서, 액체배지는 무기염류원으로서 염화나트륨, 염화칼륨, 황산마그네슘, 염화칼슘 또는 인산칼륨 중 1종 이상을 사용하는 것을 특징으로 하는 항진균성 항생물질의 생산 방법.The method for producing an antifungal antibiotic according to claim 4, wherein the liquid medium uses at least one of sodium chloride, potassium chloride, magnesium sulfate, calcium chloride or potassium phosphate as an inorganic salt source. 제4항에 있어서, 온도 25℃-35℃, 배지 pH 6-9, 호기적 조건하에서 48-96시간 진탕배양함을 특징으로 하는 항진균성 항생물질의 생산 방법.The method for producing an antifungal antibiotic according to claim 4, characterized by shaking for 48-96 hours under a temperature of 25 ° C-35 ° C, medium pH 6-9, aerobic conditions. 제4항에 있어서, 컬럼 크로마토그라피는 실리카 켈 컬럼 크로마토그라피 및 세파덱스 LH-20 컬럼 크로마토그라피인 것을 특징으로 하는 항진균성 항생물질의 생산 방법.The method of claim 4, wherein the column chromatography is silica Kel column chromatography and Sephadex LH-20 column chromatography. 제9항에 있어서, 실리카 겔 컬럼 크로마토그라피를 행할 때의 용매는 메탄올이고, 세파덱스 LH-20 컬럼크로마토그라피를 행할 때의 용매는 디클로로 메탄 : 메탄을 (1 : 5) 혼합액인 것을 특징으로 하는 항진균성 항생물질의 생산 방법.The solvent for silica gel column chromatography is methanol, and the solvent for Sephadex LH-20 column chromatography is a dichloromethane: methane (1: 5) mixed liquid. Method of producing antifungal antibiotics. 제1항의 항생물질을 유효 성분으로 하는 조성물.A composition comprising the antibiotic of claim 1 as an active ingredient. ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.※ Note: The disclosure is based on the initial application.
KR1019940019211A 1993-09-17 1994-08-03 New antifungal antibiotic and new bacillus sp. microorganism which can produce the above antibiotic and its preparing method KR970010603B1 (en)

Priority Applications (7)

Application Number Priority Date Filing Date Title
KR1019940019211A KR970010603B1 (en) 1993-09-17 1994-08-03 New antifungal antibiotic and new bacillus sp. microorganism which can produce the above antibiotic and its preparing method
CA002171947A CA2171947A1 (en) 1993-09-17 1994-09-15 New antifungal antibiotic, and new bacillus sp. microorganism which can produce the above antibiotic and its manufacturing method
PCT/KR1994/000123 WO1995007997A1 (en) 1993-09-17 1994-09-15 New antifungal antibiotic, and new bacillus sp. microorganism which can produce the above antibiotic and its manufacturing method
JP7509095A JPH09510862A (en) 1993-09-17 1994-09-15 Novel antifungal antibiotic, novel Bacillus microorganism capable of producing the antibiotic, and method for producing the same
GB9605096A GB2296716A (en) 1993-09-17 1994-09-15 New antifungal antibiotic,and new bacillus SP microorganism which can produce the above antibiotic and its manufacturing method
AU77090/94A AU7709094A (en) 1993-09-17 1994-09-15 New antifungal antibiotic, and new (bacillus sp.) microorganism which can produce the above antibiotic and its manufacturing method
CN94193799A CN1135238A (en) 1993-09-17 1994-09-15 Anti-fungal anti-biotic, and new bacillus sp. microorganism which can producing above antibiotic and method for mfg. same

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
KR93-18746 1993-09-17
KR930018746 1993-09-17
KR1019940019211A KR970010603B1 (en) 1993-09-17 1994-08-03 New antifungal antibiotic and new bacillus sp. microorganism which can produce the above antibiotic and its preparing method

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KR950008689A true KR950008689A (en) 1995-04-19
KR970010603B1 KR970010603B1 (en) 1997-06-28

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JP (1) JPH09510862A (en)
KR (1) KR970010603B1 (en)
CN (1) CN1135238A (en)
AU (1) AU7709094A (en)
CA (1) CA2171947A1 (en)
GB (1) GB2296716A (en)
WO (1) WO1995007997A1 (en)

Cited By (1)

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KR100514430B1 (en) * 2002-12-12 2005-09-14 대한민국 Isolation method of secondary product having weeding efficacy against white clover released from culture floid of sclerotinia trifoliorum bwc98-105

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CN1055310C (en) * 1995-06-22 2000-08-09 华中农业大学 Supper toxic strain YBT-1520 of thuricin brood cell and its zymosis process and products
KR100385152B1 (en) * 2001-03-14 2003-05-23 씨제이 주식회사 Process for the purification of capreomycin
WO2007016680A2 (en) * 2005-08-02 2007-02-08 University Of Missouri Board Of Curators Phage-display plant defense peptides directed against phakopsora pachyrhizi and uromyces appendiculatus
EP2098536A1 (en) 2008-03-05 2009-09-09 4-Antibody AG Isolation and identification of antigen- or ligand-specific binding proteins
CN110954625A (en) * 2019-12-21 2020-04-03 潍坊科技学院 Method for detecting antibiotic residues in soil
JP2022094481A (en) * 2020-12-15 2022-06-27 大阪瓦斯株式会社 Method for producing hydroxyalkanoic acid crystal and crystalline polymorph of hydroxyalkanoic acid

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ATE97447T1 (en) * 1987-05-30 1993-12-15 Kaken Pharma Co Ltd ANTIBIOTIC 6270B, PROCESS FOR ITS PRODUCTION AND ITS USE AS AN ANTICOCCIDIOTIC AND FEED SUPPLEMENT.
US5158937A (en) * 1988-02-08 1992-10-27 Pfizer Inc. Acidic polycyclic ether antibiotic having anticoccidial and growth promotant activity
DD270542A1 (en) * 1988-04-12 1989-08-02 Adw Ddr PROCESS FOR THE PREPARATION OF 26-DESOXYLAID LOMYCIN
US5278053A (en) * 1991-09-16 1994-01-11 Eli Lilly And Company Method of producing a polyether antibiotic from actinomadura fibrosa sp. nov. NRRL 18348 and actinomadura sp. NRRL 18880

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100514430B1 (en) * 2002-12-12 2005-09-14 대한민국 Isolation method of secondary product having weeding efficacy against white clover released from culture floid of sclerotinia trifoliorum bwc98-105

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KR970010603B1 (en) 1997-06-28
CA2171947A1 (en) 1995-03-23
GB9605096D0 (en) 1996-05-08
GB2296716A (en) 1996-07-10
CN1135238A (en) 1996-11-06
WO1995007997A1 (en) 1995-03-23
AU7709094A (en) 1995-04-03
JPH09510862A (en) 1997-11-04

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