KR940019866A - Method for producing cyclodextrin by direct microbial fermentation - Google Patents

Method for producing cyclodextrin by direct microbial fermentation Download PDF

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Publication number
KR940019866A
KR940019866A KR1019930001651A KR930001651A KR940019866A KR 940019866 A KR940019866 A KR 940019866A KR 1019930001651 A KR1019930001651 A KR 1019930001651A KR 930001651 A KR930001651 A KR 930001651A KR 940019866 A KR940019866 A KR 940019866A
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South Korea
Prior art keywords
cyclodextrin
starch
substrate
producing
regular intervals
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Application number
KR1019930001651A
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Korean (ko)
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KR960014108B1 (en
Inventor
최종수
전준현
한재춘
임성한
정갑택
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임병학
주식회사 세원
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Priority to KR1019930001651A priority Critical patent/KR960014108B1/en
Publication of KR940019866A publication Critical patent/KR940019866A/en
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Publication of KR960014108B1 publication Critical patent/KR960014108B1/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/18—Preparation of compounds containing saccharide radicals produced by the action of a glycosyl transferase, e.g. alpha-, beta- or gamma-cyclodextrins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/01—Hexosyltransferases (2.4.1)
    • C12Y204/01019—Cyclomaltodextrin glucanotransferase (2.4.1.19)

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  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Microbiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

본 발명은 미생물인 사이클로덱스트린 글루카노트랜스퍼라제를 가용성 전분 및 전분 가수분해물 발효배지에 호기적 배양을 계속하면서 일정시간 간격으로 기질을 추가하여 배양액내에 직접적으로 사이클로덱스트린을 생산하는 것에 관한 것이다.The present invention relates to the production of cyclodextrin directly in the culture medium by adding a substrate at regular intervals while continuing aerobic culture of the microbial cyclodextrin glucanotransferase in soluble starch and starch hydrolysate fermentation medium.

종래에는 전분에 상기 트랜스퍼라제를 발효배양액에서 분리정제한 후 이를 기질인 전분에 반응시켜서 사이클로덱스트린을 생산 분리하는 다단계 공정을 함으로서 공정의 복잡과 번거로움이 있었다.Conventionally, since the transferase is separated and purified from the fermentation broth in starch, and then reacted with starch as a substrate, there is a complicated and cumbersome process by performing a multi-step process of producing and separating the cyclodextrin.

본 발명은 가용성 전분 및 전분가수분해물에 사이클로덱스트린 글루카노트랜스퍼라제로서 호기적 배양을 하면서 일정시간 간격으로 기질을 추가하여 배양액내에 직접적으로 사이클로덱스트린을 생성 축적시키는 것을 특징으로 하는 사이클로덱스트린의 제조방법.The present invention is a method for producing cyclodextrin, characterized in that by adding a substrate at regular intervals while aerobic cultivation as a cyclodextrin glucanotransferase to soluble starch and starch hydrolysate to generate and accumulate cyclodextrin directly in the culture medium.

Description

미생물 직접 발효에 의한 사이클로덱스트린(Cyclodextrin)의 제조방법Method for producing cyclodextrin by direct microbial fermentation

본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음Since this is an open matter, no full text was included.

Claims (2)

사이클로덱스트린(Cyclodextrin)을 생산함에 있어서 사이클로덱스트린 글루카노트랜스퍼라제(Cyclodextrin goucanotransferase) 생성 미생물을 가용성 전분 및 전분가수분해물을 주원료로 하는 발효배지에 호기적으로 배양하면서 일정 시간 간격으로 기질을 추가하여 배양액내에 직접적으로 사이클로덱스트린을 생성 축적시키는 것을 특징으로 하는 미생물 직접발효에 의한 사이클로덱스트린의 제조방법.In the production of cyclodextrin, cyclodextrin glucanotransferase-producing microorganisms are cultured in fermentation medium containing soluble starch and starch hydrolysate as a main ingredient, and the substrate is added to the culture medium at regular intervals. A method for producing a cyclodextrin by direct fermentation of microorganisms, characterized in that the cyclodextrin is produced and accumulated directly. 제1항에 있어서 통기량을 2VVM으로 조절하면서 기질의 추가를 배양 24시간 이후 12시간 간격으로 최종농도가 4% 되도록 첨가하는 것을 특징으로 하는 사이클로덱스트린의 제조방법.The method for preparing cyclodextrin according to claim 1, wherein the addition of the substrate is added such that the final concentration is 4% at 12 hour intervals after 24 hours of incubation while controlling the aeration rate to 2VVM. ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.※ Note: The disclosure is based on the initial application.
KR1019930001651A 1993-02-08 1993-02-08 Direct fermentation process for cyclodextrin production by using microorganism KR960014108B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
KR1019930001651A KR960014108B1 (en) 1993-02-08 1993-02-08 Direct fermentation process for cyclodextrin production by using microorganism

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR1019930001651A KR960014108B1 (en) 1993-02-08 1993-02-08 Direct fermentation process for cyclodextrin production by using microorganism

Publications (2)

Publication Number Publication Date
KR940019866A true KR940019866A (en) 1994-09-15
KR960014108B1 KR960014108B1 (en) 1996-10-14

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