KR940014782A - Method of Purification of Human Growth Hormone - Google Patents

Method of Purification of Human Growth Hormone Download PDF

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Publication number
KR940014782A
KR940014782A KR1019920026040A KR920026040A KR940014782A KR 940014782 A KR940014782 A KR 940014782A KR 1019920026040 A KR1019920026040 A KR 1019920026040A KR 920026040 A KR920026040 A KR 920026040A KR 940014782 A KR940014782 A KR 940014782A
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KR
South Korea
Prior art keywords
growth hormone
human growth
purification
chromatography
ethanol precipitation
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KR1019920026040A
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Korean (ko)
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KR960013572B1 (en
Inventor
박순재
원특연
권순창
이승주
Original Assignee
최근선
주식회사 럭키
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Priority to KR1019920026040A priority Critical patent/KR960013572B1/en
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Publication of KR960013572B1 publication Critical patent/KR960013572B1/en

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

본 발명은 재조합 미생물로부터 세포 밖으로 분비된 인간 성장 호르몬을 분리 정제하는 방법에 있어서, 발효 배지에서 조정제된 인간 성장 호르몬의 수용액을 에탄올 침전시키고 침전물을 요소가 포함된 완충용액으로 용해 시킨 후 크로마토그래피를 수행함을 포함하는 인간 성장 호르몬의 정제 방법에 관한 것이다.The present invention provides a method for separating and purifying human growth hormone secreted out of cells from recombinant microorganisms, by ethanol precipitation of an aqueous solution of the adjusted human growth hormone in the fermentation medium, and the precipitate dissolved in a buffer solution containing urea, followed by chromatography It relates to a method of purifying human growth hormone comprising performing.

Description

인간 성장 호르몬의 정제 방법Method of Purification of Human Growth Hormone

본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음Since this is an open matter, no full text was included.

제4도는 본 발명의 정제 방법에 따라 얻어진 중간 생성물 및 최종 생성물을 소디움 도데실 설페이트 폴리아크릴 아마이드 겔 전기영동 (SDS-PAGE)한 결과를 나타낸 것이고,4 shows the results of sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) of the intermediate product and the final product obtained according to the purification method of the present invention,

제5도는 에탄올 첨가물을 요소를 포함한 완충용액과 요소를 포함하지 않은 완충용액을 사용하여 용해시킨 후 1차 DEAE-세파로즈 크로마토그래피한 결과를 SDS-PAGE하여 나타낸 것이다.FIG. 5 shows SDS-PAGE of the result of primary DEAE-Sepharose chromatography after dissolving the ethanol additive using a buffer containing urea and a buffer containing no urea.

Claims (5)

재조합 미생물로부터 세포 밖으로 분비된 인간 성장 호르몬을 분리 정제하는 방법에 있어서, 발효 배지에서 조정제된 인간 성장 호르몬의 수용액을 에탄올 침전시키고, 침전물을 요소가 포함된 완충용액으로 용해시킨 후 크로마토그래피를 수행함을 포함하는 인간 성장 호르몬의 정제 방법.In the method for separating and purifying human growth hormone secreted out of cells from recombinant microorganisms, an aqueous solution of the modulated human growth hormone in fermentation medium is ethanol precipitated, and the precipitate is dissolved in a buffer containing urea followed by chromatography. Purification method of a human growth hormone containing. 제1항에 있어서, 크로마토그래피는 DEAE-세파로즈(Sepharose)컬럼, 세파크릴(Sephacryl) S-200또는 S-300 컬럼 및 DEAE-세파로즈 컬럼상에서 순차적으로 실시함을 특징으로하는 방법.The method of claim 1, wherein the chromatography is performed sequentially on a DEAE-Sepharose column, a Sephacryl S-200 or S-300 column, and a DEAE-Sepharose column. 제1항에 있어서, 에탄올 침전시의 pH는 4.0 내지 6.0 임을 특징으로 하는 방법.The method according to claim 1, wherein the pH of the ethanol precipitation is 4.0 to 6.0. 제3항에 있어서, 에탄올 침전시의 염화 나트륨의 농도가 100mM 내지 1000mM임을 특징으로 하는 방법.The method according to claim 3, wherein the concentration of sodium chloride during ethanol precipitation is 100mM to 1000mM. 제1항에 있어서, 요소의 농도가 3M 내지 8M임을 특징으로 하는 방법.The method of claim 1 wherein the concentration of urea is between 3M and 8M. ※참고사항:최초출원 내용에 의하여 공개하는 것임.※ Note: This is to be disclosed based on the first application.
KR1019920026040A 1992-12-29 1992-12-29 Purification process of human growth hormone KR960013572B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
KR1019920026040A KR960013572B1 (en) 1992-12-29 1992-12-29 Purification process of human growth hormone

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR1019920026040A KR960013572B1 (en) 1992-12-29 1992-12-29 Purification process of human growth hormone

Publications (2)

Publication Number Publication Date
KR940014782A true KR940014782A (en) 1994-07-19
KR960013572B1 KR960013572B1 (en) 1996-10-09

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Application Number Title Priority Date Filing Date
KR1019920026040A KR960013572B1 (en) 1992-12-29 1992-12-29 Purification process of human growth hormone

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KR960013572B1 (en) 1996-10-09

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