KR930012781A - 10-hydroxy pheophytin a - Google Patents

Abstract

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Description

10-hydroxy pheophytin α

As this is a public information case, the full text was not included.

Figures 1a and b are thin layer chromatograms (TLC) of latent: spinach leaves, and latent: mulberry leaves, respectively.

2a, 2b and 2c show the reverse phase fast liquid chromatogram (HPLC) of band 2 shown in FIGS. 1a and b,

Reverse phase HPLC of band 2 exposed to fluorescence for several hours.

Reverse phase HPLC of band 2 exposed to light for 5 minutes.

2c reverse phase HPLC of 2 in bands not exposed to light.

FIG. 3a shows a 10-hydroxy pheophytin a (hereinafter also referred to as "CpD-1") and a ¹ H-NMR spectrum of chlorophyll a (arrow indicates the position of C ₁₀ -OH).

3b shows CpD-1 mixed with distillation (D ₂ O) and CpD-1 alone without mixing with heavy water, and ¹ H-NMR spectra of Glolofil α to measure the ¹ H resonance of C ₁₀ 'OH.

3c or ¹³ C-NMR spectrum of CpD-1.

3d or ¹ H two-dimensional COZY NMR spectrum of CpD-1.

4 shows the Fourier transform infrared spectroscopy (FT-1R) spectrum of CpD-1.

5 is a graph showing the generation of singlet oxygen at the maximum initial photooxidation rate of 1,3-diphenylisobenzofuran (DPBF) at 295 ° K.

Figure 6 is a graph showing the uptake of tumor cells and normal cells of CpD-1 and hematoporphyrin derivatives (also referred to as "HpD").

Figures 7a and 7b show survival percentages of human T-4 lymphoma cell lines (MOLT-4) and human peripheral blood lymphocytes (PBL) treated with CpD-1 and HpD and then exposed to red and yellow light, respectively. Graph showing rate.

Claims (4)

10-hydroxy fairfitin α or a pharmaceutically acceptable salt thereof represented by the following structural formula (I). (a) extracting the chlorophyll metabolite from the lactose using an appropriate solvent, (b) dissolving the extract in a suitable solvent until it is supersaturated, (c) chromatography the resulting solution to sensitize the component 10-hydroxy pheophytin a represented by the following structural formula (I), characterized in that it comprises a step of separating, and (d) collecting a fraction showing a potent photosensitizing action and purifying according to a conventional method. Method for the preparation of pharmaceutically acceptable salts. (a) reacting chlorophyll α with HCl at room temperature in ethanol or chloroform to remove magnesium ions, (b) mildly bubbling air into the resulting solution at room temperature for at least one day under dim security or in a dark C Replacing the hydrogen atom at position ₁₀ with a hydroxy group and (c) purifying the product by a conventional purification method, wherein the 10-hydroxy pheophytin a represented by formula (I) Processes for the preparation of salts which are generally acceptable. A photosensitizer composition for photoactive ammonia comprising, as an active ingredient, 10-hydroxy pheophytin a or a pharmaceutically acceptable salt thereof represented by the following structural formula (I). ※ Note: The disclosure is based on the initial application.