KR920703844A - Methods for measuring T-cell surface antigens in humans - Google Patents

Methods for measuring T-cell surface antigens in humans

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Publication number
KR920703844A
KR920703844A KR1019920701151A KR920701151A KR920703844A KR 920703844 A KR920703844 A KR 920703844A KR 1019920701151 A KR1019920701151 A KR 1019920701151A KR 920701151 A KR920701151 A KR 920701151A KR 920703844 A KR920703844 A KR 920703844A
Authority
KR
South Korea
Prior art keywords
cell surface
surface antigen
cell
subtype
analyzing
Prior art date
Application number
KR1019920701151A
Other languages
Korean (ko)
Inventor
엘. 코트진 브리안
필리파 마라크
죤 캐플러
Original Assignee
원본 미기재
내셔날 쥬이쉬 센터 포 이뮤놀러지 앤 레스피러 토리 메디신
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US07/437,370 external-priority patent/US5336598A/en
Application filed by 원본 미기재, 내셔날 쥬이쉬 센터 포 이뮤놀러지 앤 레스피러 토리 메디신 filed Critical 원본 미기재
Publication of KR920703844A publication Critical patent/KR920703844A/en

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56966—Animal cells
    • G01N33/56972—White blood cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5094—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for blood cell populations

Abstract

내용 없음No content

Description

사람에 있어 T-세포 표면 항원의 측정방법Methods for measuring T-cell surface antigens in humans

[도면의 간단한 설명][Brief Description of Drawings]

제1도는 사람 T세포의 스타필로코커스 독소 자극의 결과를 나타낸다.1 shows the results of Staphylococcus toxin stimulation of human T cells.

제2도는 독소에 의한 T세포의 Vβ 특이적 자극이 공여체 독립성이라는 사실을 보여주는 연구를 도시한다.2 shows a study showing that Vβ specific stimulation of T cells by toxin is donor independent.

제3도는 중합효소 사슬신장반응 수치(PCRs)을 혼합군에서 특정 Vβ를 가지는 T세포의 비율로 정규화하는데 이용된 표준그래프를 도시한다.3 shows the standard graph used to normalize polymerase chain extension values (PCRs) to the proportion of T cells with specific Vβ in the mixed group.

본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음Since this is an open matter, no full text was included.

Claims (10)

사람의 병리학적 상태에 특징적인 T 세포 서브타입의 수준을 측정하기 위하여 환자의 체액 샘플을 분석하고 비교가능한 체액 샘플의 정상 수준과 측정된 수준과 비교함으로써 이루어지는, 상기 T 세포 서브타입 수준의 변화가 상기 병리학적 상태를 표시하는, 사람의 병리학적 상태를 진단하는 방법.The change in the T cell subtype level, which is made by analyzing a patient's humoral sample to compare the normal and measured levels of the comparable humoral sample to determine the level of T cell subtype characteristic of the human pathological condition, Indicative of said pathological condition. 제1항에 있어서, 상기 병리학적 상태는 스타필로코커스 감염인 것을 특징으로 하는 방법.The method of claim 1, wherein the pathological condition is Staphylococcus infection. 제1항에 있어서, 상기 병리학적 상태는 자가면역 질환인 것을 특징으로 하는 방법.The method of claim 1, wherein the pathological condition is an autoimmune disease. 제1항에 있어서. 상기 T세포 서브타입에 특징적인 세포 표면 항원에 특이적으로 결합하는 항체로 상기 체액 샘플을 접촉시킴으로써 상기 체액 샘플을 분석하는 것으로 이루어지는 것을 특징으로 하는 방법.The method of claim 1. Analyzing said bodily fluid sample by contacting said bodily fluid sample with an antibody that specifically binds a cell surface antigen characteristic to said T cell subtype. 제4항에 있어서, 상기 항체는 모노클로날 항체인 것을 특징으로 하는 방법.The method of claim 4, wherein the antibody is a monoclonal antibody. 제5항에 있어서, 상기 모노클로날 항체는 Vβ5, Vβ6, Vβ8 및 Vβ12로 구성되는 군으로부터 선택된 T세포 표면 항원에 특이적인 모노클로날 항체인 것을 특징으로 하는 방법.The method of claim 5, wherein the monoclonal antibody is a monoclonal antibody specific for a T cell surface antigen selected from the group consisting of Vβ5, Vβ6, Vβ8, and Vβ12. 제1항에 있어서, T세포 표면 항원을 특징지우는, 세포 표면 항원을 코드하는 존재 DNA의 양을 측정함으로써 상기 체액 샘플을 분석하는 것으로 이루어지는 것을 특징으로 하는 방법.The method of claim 1, comprising analyzing said body fluid sample by measuring the amount of DNA present encoding a cell surface antigen that characterizes a T cell surface antigen. 제7항에 있어서, 중합효소사슬신장반응에 의하여 DNA를 측정하는 것으로 이루어지는 것을 특징으로 하는 방법.8. The method according to claim 7, wherein the DNA is measured by polymerase chain extension reaction. 제7항에 있어서, 상기 세포 표면 항원은 Vβ1, Vβ2, Vβ3, Vβ4, Vβ5.1, Vβ5.2, Vβ5.3, Vβ6.1, Vβ6.2, Vβ6.3, Vβ7, Vβ8, Vβ9, Vβ10, Vβ11, Vβ12, Vβ13.1, Vβ13.2, Vβ14, Vβ15, Vβ16, Vβ17, Vβ18, Vβ19 및 Vβ20으로 구성되는 군으로부터 선택되는 것을 특징으로 하는 방법.The method according to claim 7, wherein the cell surface antigen is Vβ1, Vβ2, Vβ3, Vβ4, Vβ5.1, Vβ5.2, Vβ5.3, Vβ6.1, Vβ6.2, Vβ6.3, Vβ7, Vβ8, Vβ9, Vβ10 , Vβ11, Vβ12, Vβ13.1, Vβ13.2, Vβ14, Vβ15, Vβ16, Vβ17, Vβ18, Vβ19 and Vβ20. 체액 Vβ2 서브타입 T세포 수준을 측정하기 위하여 환자의 체액 샘플을 분석하고 비교가능한 체액 샘플의 정상수준과 상기 수준을 비교하여 이루어지고 상기 Vβ2서브타입 수준의 증가가 독성 쇼크 신드롬을 표시하는 것을 특징으로 하는 독성 쇼크 신드롬의 진단방법.Humoral Vβ2 subtype T cell levels are analyzed by analyzing a patient's humoral sample and comparing the normal and comparable levels of the comparable humoral sample, wherein the increase in the Vβ2 subtype level indicates a toxic shock syndrome. Method of diagnosis of toxic shock syndrome. ※ 참고사항 : 최초출원 내용에 의하여 공개되는 것임.※ Note: This is to be disclosed by the original application.
KR1019920701151A 1989-11-15 1990-11-13 Methods for measuring T-cell surface antigens in humans KR920703844A (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US07/437,370 US5336598A (en) 1989-11-15 1989-11-15 Method for diagnosing a superantigen caused pathologial condition via assay of T-cells
US437,370 1989-11-15
US48835390A 1990-03-02 1990-03-02
US488,353 1990-03-02
PCT/US1990/006613 WO1991007508A1 (en) 1989-11-15 1990-11-13 Method for measuring t-cell surface antigens in humans

Publications (1)

Publication Number Publication Date
KR920703844A true KR920703844A (en) 1992-12-18

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Application Number Title Priority Date Filing Date
KR1019920701151A KR920703844A (en) 1989-11-15 1990-11-13 Methods for measuring T-cell surface antigens in humans

Country Status (7)

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EP (1) EP0500780A4 (en)
JP (1) JPH05504621A (en)
KR (1) KR920703844A (en)
AU (1) AU651346B2 (en)
CA (1) CA2068888A1 (en)
FI (1) FI922197A (en)
WO (1) WO1991007508A1 (en)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0602178A4 (en) * 1991-08-28 1995-10-25 Wistar Inst T cell receptor-based therapy for rheumatoid arthritis.
FR2736831B1 (en) * 1995-07-19 1997-08-22 Centre Nat Rech Scient NUCLEOTIDE AND PEPTIDE SEQUENCES FOR THE TREATMENT OF MYASTHENIA
WO1998054575A2 (en) * 1997-05-27 1998-12-03 University Of Wales College Of Medicine Method for assessing wound healing
WO2016193299A1 (en) 2015-06-01 2016-12-08 Medigene Immunotherapies Gmbh T cell receptor library
AU2016273214B2 (en) 2015-06-01 2018-10-18 Medigene Immunotherapies Gmbh Method for generating antibodies against T cell receptor
NZ737851A (en) * 2015-06-01 2019-08-30 Medigene Immunotherapies Gmbh T-cell receptor specific antibodies
PL3394247T3 (en) 2015-12-23 2021-08-23 Medigene Immunotherapies Gmbh Novel generation of antigen-specific tcrs

Family Cites Families (8)

* Cited by examiner, † Cited by third party
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US4677061A (en) * 1984-10-19 1987-06-30 Genetic Systems Corporation T-cell lymphocyte subset monitoring of immunologic disease
US4683195A (en) * 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4667061A (en) * 1985-04-02 1987-05-19 Hitachi, Ltd. Gas insulated apparatus with internal coated insulation layer of high dielectric constant
US4886743A (en) * 1985-04-24 1989-12-12 California Institute Of Technology Diagnostic reagents based on unique sequences within the variable region of the T cell receptor and uses thereof
US5340921A (en) * 1986-07-03 1994-08-23 T Cell Sciences, Inc. Γ, δT cell receptor and methods and detection
CA1305912C (en) * 1987-06-22 1992-08-04 Richard J. Albertini Detection of lymphocyte amplification
FI891226A (en) * 1988-04-28 1989-10-29 Univ Leland Stanford Junior RESEPTORDETERMINANTER I ANTI-T-CELLER FOER BEHANDLING AV AUTOIMMUNSJUKDOM.
WO1990004180A1 (en) * 1988-10-06 1990-04-19 T Cell Sciences, Inc. Therapeutic and diagnostic methods using soluble t cell surface molecules

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CA2068888A1 (en) 1991-05-16
FI922197A0 (en) 1992-05-14
AU7787391A (en) 1991-06-13
AU651346B2 (en) 1994-07-21
EP0500780A1 (en) 1992-09-02
WO1991007508A1 (en) 1991-05-30
EP0500780A4 (en) 1993-03-10
JPH05504621A (en) 1993-07-15
FI922197A (en) 1992-05-14

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