KR920014935A - Amplification method by chain substitution - Google Patents

Amplification method by chain substitution Download PDF

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Publication number
KR920014935A
KR920014935A KR1019920001344A KR920001344A KR920014935A KR 920014935 A KR920014935 A KR 920014935A KR 1019920001344 A KR1019920001344 A KR 1019920001344A KR 920001344 A KR920001344 A KR 920001344A KR 920014935 A KR920014935 A KR 920014935A
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South Korea
Prior art keywords
nucleic acid
acid sequence
sequence
reaction mixture
reaction
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KR1019920001344A
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Korean (ko)
Inventor
티. 월커 조지
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레이몬드 피. 올뮬러
벡톤, 디킨슨 앤드 컴퍼니
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Priority to KR1019920001344A priority Critical patent/KR920014935A/en
Publication of KR920014935A publication Critical patent/KR920014935A/en

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Abstract

내용 없음No content

Description

사슬 치환에 의한 증폭방법Amplification method by chain substitution

본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음Since this is an open matter, no full text was included.

제1도는 일본쇄(single stranded) DNA 단편에 대한 본 발명의 방법예를 단계적으로 도식화한 것이다, 제2도는 이본쇄(double stranded) 게놈 DNA에 대한 본 발명의 방법예를 단계적으로 도식화한 것이다.FIG. 1 shows a step by step diagram of the method of the invention for single stranded DNA fragments. FIG. 2 shows a step by step diagram of the method example of the invention for double stranded genomic DNA.

Claims (2)

a)과량의 데옥시누클레오사이드트리포스페이트(이것중 최소한 하나는 치환됨), 5'→3'엑소투클레아제 활성이 없는 DNA 중합효소, 타겟 서열의 일본쇄에 상보적이고 5'말단에서 엔도누크레아제에 대한 인지 서열을 갖는 프라이머, 및 프로브의 인지 서열을 분해할 수 있는 엔도누클레아제를 포함한 반응 혼합물을 타겟 핵산 서열에 부가하고, b)반응 생성물을 생성시키기에 충분한 시간 동안 상기 반응 혼합물을 일본쇄 단편과 반응시키는 단계들로 구성된, 타겟 핵산 서열의 증폭방법.a) Excess deoxynucleoside triphosphate (at least one of which is substituted), DNA polymerase without 5 '→ 3' exotoclease activity, complementary to the Japanese chain of the target sequence and endo at the 5 'end A reaction mixture comprising a primer having a recognition sequence for nuclease and an endonuclease capable of cleaving the recognition sequence of the probe is added to the target nucleic acid sequence, b) the reaction for a time sufficient to produce a reaction product. A method of amplifying a target nucleic acid sequence, consisting of reacting the mixture with a single chain fragment. a)카핑될 핵산 서열의 하나 이상의 일본쇄 단편을 제조하고, b)과량의 데옥시누클레오사이드트리포스페이트(이것중 최소한 하나는 치환됨), 5'→3' 엑소누클레아제 활성이 없는 DNA 중합효소, 타겟 서열의 일본쇄에 상보적이고 5'말단에서 엔도누클레아제를 포함한 반응 혼합물을 부가하며, c)반응 생성물을 생성시키기에 충분한 시간 동안 상기 반응 혼합물을 일본쇄 단편과 반응시키는 단계들로 구성된, 단일 핵산 서열의 다수의 카피를 제조하는 방법.a) preparing one or more single chain fragments of the nucleic acid sequence to be capped, b) excess deoxynucleoside triphosphate (at least one of which is substituted), DNA without 5 '→ 3' exonuclease activity Adding a polymerase, a reaction mixture complementary to the Japanese chain of the target sequence and comprising an endonuclease at the 5 'end, and c) reacting the reaction mixture with the Japanese chain fragment for a time sufficient to produce a reaction product. Consisting of a plurality of copies of a single nucleic acid sequence. ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.※ Note: The disclosure is based on the initial application.
KR1019920001344A 1991-01-31 1992-01-30 Amplification method by chain substitution KR920014935A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
KR1019920001344A KR920014935A (en) 1991-01-31 1992-01-30 Amplification method by chain substitution

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US91-07/648,2 1991-01-31
KR1019920001344A KR920014935A (en) 1991-01-31 1992-01-30 Amplification method by chain substitution

Publications (1)

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KR920014935A true KR920014935A (en) 1992-08-25

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KR1019920001344A KR920014935A (en) 1991-01-31 1992-01-30 Amplification method by chain substitution

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KR (1) KR920014935A (en)

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