KR920012427A - Method for preparing alpha glucosyl transferase from microorganisms - Google Patents

Method for preparing alpha glucosyl transferase from microorganisms Download PDF

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Publication number
KR920012427A
KR920012427A KR1019900021218A KR900021218A KR920012427A KR 920012427 A KR920012427 A KR 920012427A KR 1019900021218 A KR1019900021218 A KR 1019900021218A KR 900021218 A KR900021218 A KR 900021218A KR 920012427 A KR920012427 A KR 920012427A
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KR
South Korea
Prior art keywords
microorganisms
corn steep
steep liquor
alpha glucosyl
glucosyl transferase
Prior art date
Application number
KR1019900021218A
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Korean (ko)
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KR920008371B1 (en
Inventor
김정환
김을수
전영중
고중환
현형환
Original Assignee
김정순
제일제당 주식회사
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Priority to KR1019900021218A priority Critical patent/KR920008371B1/en
Publication of KR920012427A publication Critical patent/KR920012427A/en
Application granted granted Critical
Publication of KR920008371B1 publication Critical patent/KR920008371B1/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
    • A61K8/66—Enzymes
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q11/00—Preparations for care of the teeth, of the oral cavity or of dentures; Dentifrices, e.g. toothpastes; Mouth rinses
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • C07K14/27—Erwinia (G)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/18—Preparation of compounds containing saccharide radicals produced by the action of a glycosyl transferase, e.g. alpha-, beta- or gamma-cyclodextrins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/01—Hexosyltransferases (2.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/18—Erwinia

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Veterinary Medicine (AREA)
  • Molecular Biology (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Birds (AREA)
  • Epidemiology (AREA)
  • Biophysics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Oral & Maxillofacial Surgery (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

내용 없음No content

Description

미생물로 부터 알파글루코실트랜스퍼라제의 제조방법Method for preparing alpha glucosyl transferase from microorganisms

본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음Since this is an open matter, no full text was included.

Claims (4)

어위니아라폰티치 종에 속하는 미생물을 설탕 또는 과당 효모추출물, 옥수수침지액, 제2인산나트륨을 주성분으로 하는 배양배지에서 호기적으로 배양하여 알파글루코실트랜스퍼라제를 생산함을 특징으로하는 알파 글루코실트랜스퍼라제의 제조방법.Alpha glucosyl, characterized in that the microorganism belonging to the species of Erwinia rapponti is aerobically cultured in a culture medium containing sugar or fructose yeast extract, corn steep liquor and dibasic sodium phosphate as the main components to produce alpha glucosyl transferase. Method for producing transferase. 제1항에 있어서, 배양배지는 옥수수침지액이 제거된 것임을 특징으로 하는 방법.The method of claim 1, wherein the culture medium is characterized in that the corn steep liquor is removed. 제1항에 있어서, 배지조성은 설탕 40-100g/ℓ, 효모추출물 5-20ℓ/g, 옥수수 침지액15-120㎖/ℓ, 제2인 산나트륨 12-16mM로 하는 것을 특징으로 하는 방법.The method of claim 1, wherein the medium composition is characterized in that the sugar 40-100g / l, yeast extract 5-20l / g, corn steep liquor 15-120ml / l, the second sodium acid 12-16mM. 제1항에 있어서, 배양온도를 20-25℃로 하고, 배양 pH를 5.5-6.5로 조절하는 것을 특징으로 하는 방법.The method of claim 1 wherein the incubation temperature is 20-25 ° C. and the culture pH is adjusted to 5.5-6.5. ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.※ Note: The disclosure is based on the initial application.
KR1019900021218A 1990-12-20 1990-12-20 Method for producing alpha-glucosyltransferase KR920008371B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
KR1019900021218A KR920008371B1 (en) 1990-12-20 1990-12-20 Method for producing alpha-glucosyltransferase

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR1019900021218A KR920008371B1 (en) 1990-12-20 1990-12-20 Method for producing alpha-glucosyltransferase

Publications (2)

Publication Number Publication Date
KR920012427A true KR920012427A (en) 1992-07-27
KR920008371B1 KR920008371B1 (en) 1992-09-26

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Family Applications (1)

Application Number Title Priority Date Filing Date
KR1019900021218A KR920008371B1 (en) 1990-12-20 1990-12-20 Method for producing alpha-glucosyltransferase

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KR (1) KR920008371B1 (en)

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Publication number Publication date
KR920008371B1 (en) 1992-09-26

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