KR900702012A - Preparation of thermally stable alpha-amylase - Google Patents

Preparation of thermally stable alpha-amylase

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Publication number
KR900702012A
KR900702012A KR1019900700360A KR900700360A KR900702012A KR 900702012 A KR900702012 A KR 900702012A KR 1019900700360 A KR1019900700360 A KR 1019900700360A KR 900700360 A KR900700360 A KR 900700360A KR 900702012 A KR900702012 A KR 900702012A
Authority
KR
South Korea
Prior art keywords
medium
culture
carbon source
amylase
main carbon
Prior art date
Application number
KR1019900700360A
Other languages
Korean (ko)
Inventor
제임스 씨 켄
윌리엄 알 람
프란시스 엠. 라신
할 알. 투르너
Original Assignee
데이비드 에이, 핼
그레이트 레이크스 케미컬 코포레이션
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by 데이비드 에이, 핼, 그레이트 레이크스 케미컬 코포레이션 filed Critical 데이비드 에이, 핼
Publication of KR900702012A publication Critical patent/KR900702012A/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2414—Alpha-amylase (3.2.1.1.)
    • C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

내용 없음No content

Description

열적으로 안정한 알파-아밀라아제의 제법Preparation of thermally stable alpha-amylase

본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음Since this is an open matter, no full text was included.

Claims (10)

보통 알파-아밀라아제의 합성을 억제하는 탄수화물을 주탄소공급원으로서 함유하는 영양배지에서 비이, 리케니 포르미스 ATCC 제 53,772번을 배양하고, 배양동안 주탄소공급원을 배지에 공급하고, 배양동안 주탄소공급원이 그 배지에 공급되는 비율을 조종함으로써 0.5 내지 1.0 공기부피/배지부피/분 사이의 공기흐름에서 배양동안에 그 배지로부터 방출되는 배출가스중의 이산화탄소 수준을 4.5%와 6.5%사이로 유지하며, 그리고 배지의 pH를 6.5와 7.0사이로 유지하는 단계들로 구성되는 열적으로 안정한 알파-아밀라아제의 제조방법.Incubate B. rickenformis ATCC No. 53,772 in a nutrient medium containing carbohydrates, which normally inhibit the synthesis of alpha-amylase, as the main carbon source, supply the main carbon source to the medium during the culture, and the main carbon source during the culture. By controlling the rate supplied to the medium, the carbon dioxide levels in the off-gases emitted from the medium during the cultivation are maintained between 4.5% and 6.5% in an air flow between 0.5 and 1.0 air volume / medium volume / minute, and the medium A method of preparing a thermally stable alpha-amylase comprising the steps of maintaining a pH of between 6.5 and 7.0. 제1항에 있어서, 주탄소공급원은 덱스트로오스, 녹말 가수분해물, 글리세롤, 크실로오, 만로오스, 수크로우스 및 이들의 혼합물로 이루어진 그룹에서 선택됨을 특징으로 하는 방법.The method of claim 1, wherein the main carbon source is selected from the group consisting of dextrose, starch hydrolyzate, glycerol, xyllo, manrose, sucrose and mixtures thereof. 제1항에 있어서, 영양배지는 콩가루 옥수수침지액, 목화씨가루, 효모추출물, 땅콩 깻묵, 가용성 질산염, 암모늄염 및 이들의 혼합물들로 이루어진 그룹에서 선택된 물질을 질소공급원으로서 함유함을 특징으로 하는 방법.The method of claim 1 wherein the nutrient medium contains as a nitrogen source a substance selected from the group consisting of soy flour corn steep liquor, cottonseed flour, yeast extract, peanut wax, soluble nitrate, ammonium salt and mixtures thereof. 제2항에 있어서, 영양배는 콩가루, 옥수수 침지액, 목화씨 가루,효모 추출물, 땅콩 깻묵, 가용성 질산염, 암모늄염 및 이들의 혼합물들로 이루어진 그룹에서 선택된 물질을 질소공급원으로서 함유함을 특징으로 하는 방법.The method of claim 2, wherein the nutrient embryo contains a substance selected from the group consisting of soy flour, corn steep liquor, cottonseed flour, yeast extract, peanut jelly, soluble nitrate, ammonium salt and mixtures thereof as a nitrogen source. . 제1항에 있어서, 그 배양을 72 내지 144시간동안 수행함을 특징으로 하는 방법.The method of claim 1, wherein the culture is carried out for 72 to 144 hours. 제1항에 있어서, 그 배양을 38℃내지 44℃이 온도에서 수행함을 특징으로 하는 방법.The method of claim 1, wherein the culturing is performed at a temperature of 38 ° C to 44 ° C. 제1항에 있어서, 그 배지의 pH는 암모니아를 부가하여 6.5와 7.0사이로 유지함을 특징으로 하는 방법.The method of claim 1 wherein the pH of the medium is maintained between 6.5 and 7.0 by adding ammonia. 제2항에 있어서, 그 배양을 72내지 144시간동안 수행함을 특징으로 하는 방법.The method of claim 2, wherein the culture is carried out for 72 to 144 hours. 제2항에 있어서, 그 배양을 38℃내지 44℃이 온도에서 수행함을 특징으로 하는 방법.The method of claim 2, wherein the culturing is performed at a temperature of 38 ° C. to 44 ° C. 4. 제2항에 있어서, 그 배지의 pH는 암모니아를 부가하여 6.5와 7.0사이로 유지함을 특징으로 하는 방법.The method of claim 2, wherein the pH of the medium is maintained between 6.5 and 7.0 by adding ammonia. ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.※ Note: The disclosure is based on the initial application.
KR1019900700360A 1988-06-21 1989-06-19 Preparation of thermally stable alpha-amylase KR900702012A (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US20942688A 1988-06-21 1988-06-21
US209.426 1988-06-21
PCT/US1989/002683 WO1989012679A1 (en) 1988-06-21 1989-06-19 Method for producing thermally stable alpha-amylase

Publications (1)

Publication Number Publication Date
KR900702012A true KR900702012A (en) 1990-12-05

Family

ID=22778710

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1019900700360A KR900702012A (en) 1988-06-21 1989-06-19 Preparation of thermally stable alpha-amylase

Country Status (9)

Country Link
EP (1) EP0380617A4 (en)
JP (1) JPH03501328A (en)
KR (1) KR900702012A (en)
BR (1) BR8906981A (en)
DK (1) DK46090D0 (en)
ES (1) ES2012737A6 (en)
FI (1) FI900838A0 (en)
HU (1) HUT53934A (en)
WO (1) WO1989012679A1 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP5746614B2 (en) 2008-04-15 2015-07-08 ザ ユナイテッド ステイツ オブ アメリカ、 アズ リプレゼンティッド バイ ザ セクレタリー オブ アグリカルチャー Protein concentrate obtained from starch-containing grains, its composition, production method and use

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4473645A (en) * 1983-03-30 1984-09-25 Nabisco Brands, Inc. Process for producing thermally stable alpha-amylase
ES8602115A1 (en) * 1983-10-11 1985-11-01 Espanola Petrol A process for producing thermostable alpha-amylases by culturing micro-organisms at elevated temperatures.

Also Published As

Publication number Publication date
ES2012737A6 (en) 1990-04-01
EP0380617A1 (en) 1990-08-08
BR8906981A (en) 1990-12-18
FI900838A0 (en) 1990-02-20
HU894144D0 (en) 1990-09-28
HUT53934A (en) 1990-12-28
DK46090A (en) 1990-02-21
EP0380617A4 (en) 1991-09-25
DK46090D0 (en) 1990-02-21
WO1989012679A1 (en) 1989-12-28
JPH03501328A (en) 1991-03-28

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