KR900018676A - Methods of quantitating immunologically testable substances and reaction vessels suitable for them - Google Patents

Methods of quantitating immunologically testable substances and reaction vessels suitable for them Download PDF

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KR900018676A
KR900018676A KR1019890006994A KR890006994A KR900018676A KR 900018676 A KR900018676 A KR 900018676A KR 1019890006994 A KR1019890006994 A KR 1019890006994A KR 890006994 A KR890006994 A KR 890006994A KR 900018676 A KR900018676 A KR 900018676A
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South Korea
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avidin
streptavidin
reaction
vessel
bound
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KR1019890006994A
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KR910008704B1 (en
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슈미트 우르반
마우레르 에버하드
뢰딘거 볼프강
데그 롤프
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데에르.포우크에트. 데에르. 베버
뵈링거 만하임 게임베하
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Peptides Or Proteins (AREA)

Abstract

내용 없음No content

Description

면역학적으로 검사가능한 물질의 정량 방법 및 이것에 적당한 반응 용기Methods of quantitating immunologically testable substances and reaction vessels suitable for them

본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음Since this is an open matter, no full text was included.

제1도는 TSH 정량에 대한 여러 캘리브레이션 커브(calibration curve)를 표시한다. 제2도는 프롤락틴 테스트에 대한 캘리브레이션 커브를 표시한다. 제3도는 CEA-테스트에 대한 캘리브레이션 커브를 표시한다.Figure 1 shows the various calibration curves for TSH quantification. 2 shows the calibration curve for the prolactin test. 3 shows the calibration curve for the CEA-test.

Claims (8)

내부 표면에 스트렙타비딘이나 아비딘이 반응 부피 ml당 0.1-2.5㎍존재하는 양으로 결합되어 있는 고체상으로서 반응 용기가 사용되는 것을 특징으로 하는, 면역학적으로 활성인 반응 성분의 하나가 결합된 고체상을 사용함에 의해 이종 면역분석에 기초하는 면역학적으로 검사가능한 물질을 정량하는 방법.A solid phase in which one of the immunologically active reaction components is bound is characterized in that the reaction vessel is used as a solid phase in which streptavidin or avidin is bound in an amount of 0.1-2.5 µg per ml of reaction volume on the inner surface. A method of quantifying an immunologically testable substance based on a heterogeneous immunoassay. 제1항에 있어서, 큐베트(cuvette)가 월(wall)의 내부 표면이 스트렙타비딘이나 아비딘으로 피복된 반응 용기로서 사용되는 방법.The method of claim 1, wherein the cuvette is used as a reaction vessel in which the inner surface of the wall is covered with streptavidin or avidin. 전기한 항의 어느 한 항에 있어서, 스트렙타비딘 또는 아비딘이 약 500000 이상의 분자량을 갖는 가용성 단백질에 결합된 다음 스트렙타비딘이나 아비딘과 단백질의 결합체가 소수성 고체상에 흡착되는 방법.The method of claim 1, wherein streptavidin or avidin is bound to a soluble protein having a molecular weight of at least about 500000 and then the streptavidin or a combination of avidin and the protein is adsorbed onto a hydrophobic solid phase. 전기한 항의 어느 한 항에 있어서, 특별한 여러 항체내 여러 가지 물질이 동시에 정량되는 방법.The method according to any one of the preceding claims, wherein different substances in different specific antibodies are quantified simultaneously. 용기의 내부 공간이 액체에 대한 용기로서 계획되고 피복물의 스트렙타비딘이나 아비딘 각각의 함량은 반응 부피 ml당 0.1-2.5㎍의 스트렙타비딘 또는 아비딘이 존재하도록 조화되어 있는 서로 면해있는 광학적으로 투명한 월 부분과 액체에 대한 용기로서 계획된 내부 월 부분내에 최소한 부분적으로 있는 아비딘이나 스트렙타비딘으로 피복된 월을 지닌 반응 용기.Optically transparent walls facing each other in which the inner space of the vessel is designed as a vessel for liquid and the content of streptavidin or avidin in the coating is coordinated such that 0.1-2.5 μg of streptavidin or avidin is present per ml of reaction volume. A reaction vessel having a wall coated with avidin or streptavidin at least partially within the planned inner wall portion as a vessel for the portion and liquid. 제5항에 있어서, 스트렙타비딘 또는 아비딘이 약 500000 이상의 분자량을 갖는 가용성 단백질에 의해 고체상에 결합되는 반응용기.The reaction vessel of claim 5, wherein streptavidin or avidin is bound to the solid phase by a soluble protein having a molecular weight of at least about 500000. 7. 제5항 또는 제6항에 있어서, 반응 용기가 큐베트인 반응 용기.The reaction vessel according to claim 5 or 6, wherein the reaction vessel is a cuvette. 테스트 반응에 대해 반응 부피ml당 0.1-2.5㎍의 스트렙타비딘이나 아비딘이 내부 표면에 결합되어 있는 용기로 구성되고 면역분석에 기초한 방법을 이용해 다른 파라미터를 정량하기 위한 표준 반응 용기의 사용방법.Use of standard reaction vessels to quantify other parameters using a method based on immunoassay, consisting of a vessel in which 0.1-2.5 μg streptavidin or avidin is bound to the inner surface per ml of reaction for a test reaction. ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.※ Note: The disclosure is based on the initial application.
KR1019890006994A 1988-05-25 1989-05-25 Process for the determination of an immunologically dectection substance and a suitable reaction vessel therefor KR910008704B1 (en)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
DEP3817716.1 1988-05-25
DE3817716 1988-05-25
DEP3901638.2 1989-01-20
DE3901638A DE3901638C2 (en) 1988-05-25 1989-01-20 Method for determining an immunologically detectable substance and suitable reaction vessel

Publications (2)

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KR900018676A true KR900018676A (en) 1990-12-22
KR910008704B1 KR910008704B1 (en) 1991-10-19

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KR (1) KR910008704B1 (en)
DD (1) DD283867A5 (en)
DE (1) DE3901638C2 (en)
ZA (1) ZA893909B (en)

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* Cited by examiner, † Cited by third party
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DE102007017681A1 (en) * 2007-04-14 2009-01-08 Papst Licensing Gmbh & Co. Kg Device for determining activity of enzymes in liquid test sample containing enzyme and enzyme inhibitor, comprises chromatography column, which contains substrate and substance bound to substrate

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4935339A (en) * 1985-05-07 1990-06-19 Nichols Institute Diagnostics Delayed solid phase immunologic assay
FR2601455B1 (en) * 1986-07-11 1989-08-04 Stallergenes Lab NEW SOLID-PHASE BIOLOGICAL REAGENTS AND THEIR PROCESS FOR OBTAINING
DE3640412A1 (en) * 1986-11-26 1988-06-09 Boehringer Mannheim Gmbh METHOD FOR DETERMINING A SPECIFICALLY BINDABLE SUBSTANCE

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DD283867A5 (en) 1990-10-24
DE3901638C2 (en) 1999-03-25
DE3901638A1 (en) 1989-12-07
ZA893909B (en) 1990-02-28
KR910008704B1 (en) 1991-10-19

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